دورية أكاديمية
A Comparative and Critical Analysis for In Vitro Cytotoxic Evaluation of Magneto-Crystalline Zinc Ferrite Nanoparticles Using MTT, Crystal Violet, LDH, and Apoptosis Assay.
| العنوان: | A Comparative and Critical Analysis for In Vitro Cytotoxic Evaluation of Magneto-Crystalline Zinc Ferrite Nanoparticles Using MTT, Crystal Violet, LDH, and Apoptosis Assay. |
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| المؤلفون: | de la Fuente-Jiménez JL; Centre of Bioengineering, School of Engineering and Sciences, Tecnologico de Monterrey, Campus Queretaro, Av. Epigmenio González, No. 500, Fracc. San Pablo, Querétaro 76130, Mexico., Rodríguez-Rivas CI; División de Investigación y Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro, Cerro de Las Campanas S/N, Querétaro 76010, Mexico., Mitre-Aguilar IB; Unidad de Bioquímica, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga 15, Belisario Domínguez secc 16, Tlalpan, Mexico City 14080, Mexico., Torres-Copado A; Centre of Bioengineering, School of Engineering and Sciences, Tecnologico de Monterrey, Campus Queretaro, Av. Epigmenio González, No. 500, Fracc. San Pablo, Querétaro 76130, Mexico., García-López EA; Unidad de Bioquímica, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga 15, Belisario Domínguez secc 16, Tlalpan, Mexico City 14080, Mexico., Herrera-Celis J; Centro de Investigación y Desarrollo Tecnológico en Electroquímica, Parque Tecnológico, Sanfandila, Pedro Escobedo, Querétaro 76703, Mexico., Arvizu-Espinosa MG; Centre of Bioengineering, School of Engineering and Sciences, Tecnologico de Monterrey, Campus Queretaro, Av. Epigmenio González, No. 500, Fracc. San Pablo, Querétaro 76130, Mexico., Garza-Navarro MA; Facultad de Ingeniería Mecánica y Eléctrica, FIME, Universidad Autónoma de Nuevo León, UANL, Av. Universidad S/N, Cd. Universitaria, San Nicolás de los Garza 66450, Mexico., Arriaga LG; Centro de Investigación y Desarrollo Tecnológico en Electroquímica, Parque Tecnológico, Sanfandila, Pedro Escobedo, Querétaro 76703, Mexico., García JL; División de Investigación y Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro, Cerro de Las Campanas S/N, Querétaro 76010, Mexico., García-Gutiérrez DI; Facultad de Ingeniería Mecánica y Eléctrica, FIME, Universidad Autónoma de Nuevo León, UANL, Av. Universidad S/N, Cd. Universitaria, San Nicolás de los Garza 66450, Mexico., Dehesa AZ; Unidad de Bioquímica, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga 15, Belisario Domínguez secc 16, Tlalpan, Mexico City 14080, Mexico.; Departamento de Medicina Genómica y Toxicología Ambiental, Programa Institucional de Cáncer de Mama, Instituto de Investigaciones Biomédicas IIB & Red de Apoyo a la Investigación, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ave. Universidad 3000, Col. Copilco Universidad, Del. Coyoacán Ciudad de México, Mexico City 04510, Mexico., Sharma A; Centre of Bioengineering, School of Engineering and Sciences, Tecnologico de Monterrey, Campus Queretaro, Av. Epigmenio González, No. 500, Fracc. San Pablo, Querétaro 76130, Mexico., Oza G; Centro de Investigación y Desarrollo Tecnológico en Electroquímica, Parque Tecnológico, Sanfandila, Pedro Escobedo, Querétaro 76703, Mexico. |
| المصدر: | International journal of molecular sciences [Int J Mol Sci] 2023 Aug 16; Vol. 24 (16). Date of Electronic Publication: 2023 Aug 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2000- |
| مواضيع طبية MeSH: | Triple Negative Breast Neoplasms* , Antineoplastic Agents* , Adenocarcinoma*, Humans ; Gentian Violet ; Zinc ; HEK293 Cells ; Apoptosis |
| مستخلص: | Zinc ferrite nanoparticles (ZFO NPs) are a promising magneto-crystalline platform for nanomedicine-based cancer theranostics. ZFO NPs synthesized using co-precipitation method are characterized using different techniques. UV-visible spectroscopy exhibits absorption peaks specific for ZFO. Raman spectroscopy identifies Raman active, infrared active, and silent vibrational modes while Fourier transforms infrared spectroscopic (FTIR) spectra display IR active modes that confirm the presence of ZFO. X-ray diffraction pattern (XRD) exhibits the crystalline planes of single-phase ZFO with a face-centered cubic structure that coincides with the selected area electron diffraction pattern (SAED). The average particle size according to high-resolution transmission electron microscopy (HR-TEM) is 5.6 nm. X-ray photoelectron spectroscopy (XPS) signals confirm the chemical states of Fe, Zn, and O. A superconducting quantum interference device (SQUID) displays the magnetic response of ZFO NPs, showing a magnetic moment of 45.5 emu/gm at 70 kOe. These ZFO NPs were then employed for comparative cytotoxicity evaluation using MTT, crystal violet, and LDH assays on breast adenocarcinoma epithelial cell (MCF-7), triple-negative breast cancer lines (MDA-MB 231), and human embryonic kidney cell lines (HEK-293). Flow cytometric analysis of all the three cell lines were performed in various concentrations of ZFO NPs for automated cell counting and sorting based on live cells, cells entering in early or late apoptotic phase, as well as in the necrotic phase. This analysis confirmed that ZFO NPs are more cytotoxic towards triple-negative breast cancer cells (MDA-MB-231) as compared to breast adenocarcinoma cells (MCF-7) and normal cell lines (HEK-293), thus corroborating that ZFO can be exploited for cancer therapeutics. |
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| معلومات مُعتمدة: | Ciencias de Fronteras CF/19-2096029 Consejo Nacional de Ciencia y Tecnología; 321116 Laboratorio Nacional micro y nanofluidica-LABMyN |
| فهرسة مساهمة: | Keywords: LDH; MTT; apoptosis; co-precipitation; cytotoxicity; flow cytometry; magneto-crystalline; triple-negative breast cancer; zinc ferrite |
| المشرفين على المادة: | J4Z741D6O5 (Gentian Violet) 1317-54-0 (ferrite) J41CSQ7QDS (Zinc) 0 (Antineoplastic Agents) |
| تواريخ الأحداث: | Date Created: 20230826 Date Completed: 20230828 Latest Revision: 20230829 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC10454666 |
| DOI: | 10.3390/ijms241612860 |
| PMID: | 37629040 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1422-0067 |
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| DOI: | 10.3390/ijms241612860 |