دورية أكاديمية
Bone strength is reduced in a neonatal androgenized rat model.
| العنوان: | Bone strength is reduced in a neonatal androgenized rat model. |
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| المؤلفون: | Mujica LKS; Animal Science Department, Cooperative University of Colombia (UCC), Bucaramanga, Colombia., Dos Santos Amaral C; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, Brazil., Valente FS; Animal Care Facility, Federal University of Santa Maria (UFSM), Santa Maria, Brazil., Miyazato LG; Animal Care Facility, Federal University of Santa Maria (UFSM), Santa Maria, Brazil., Macari S; Department of Oral Pathology and Surgery, Faculty of Dentistry, Federal University of Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil., da Silva TA; Department of Oral Pathology and Surgery, Faculty of Dentistry, Federal University of Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil., Barrioni BR; Department of Metallurgical and Materials Engineering, Faculty of Engineering, Federal University of Minas Gerais, Belo Horizonte (UFMG), Minas Gerais, Brazil., Carlos BL; Department of Biomechanics, Medicine and Locomotor Apparatus Rehabilitation, Faculty of Medicine, University of Sao Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Silva GJA; Department of Biomechanics, Medicine and Locomotor Apparatus Rehabilitation, Faculty of Medicine, University of Sao Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Shimano AC; Department of Biomechanics, Medicine and Locomotor Apparatus Rehabilitation, Faculty of Medicine, University of Sao Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Antoniazzi AQ; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, Brazil., Premaor MO; Department of Clinical Medicine, Faculty of Medicine, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., Comim FV; Department of Clinical Medicine, Faculty of Medicine, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil. |
| المصدر: | Bone reports [Bone Rep] 2023 Aug 19; Vol. 19, pp. 101710. Date of Electronic Publication: 2023 Aug 19 (Print Publication: 2023). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc Country of Publication: United States NLM ID: 101646176 Publication Model: eCollection Cited Medium: Print ISSN: 2352-1872 (Print) Linking ISSN: 23521872 NLM ISO Abbreviation: Bone Rep Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: [Philadelphia] : Elsevier Inc., [2015]- |
| مستخلص: | Background: Whether polycystic ovary syndrome (PCOS) affects bone health during a woman's lifespan remains controversial. An androgenized rodent model replicated many metabolic and reproductive features of women with PCOS, and we aimed to use it to investigate the impact of androgens on microarchitecture (by micro-CT), bone mechanical strength, bone formation and resorption markers in rats with intact ovaries (SHAM) who underwent oophorectomy. Methods: Wistar rats ( Rattus norvegicus albinus) were employed for the experiments in this study. The protocol of androgenization consisted of the application of 1.25 mg s.c. testosterone propionate beteween days 2-5 of life, while the controls received the same amount of corn oil s.c. as previously established. Androgenized SHAM rats exhibited chronic anovulation identified by vaginal cytology and a reduction in the proportion of corpus luteum in the ovary in comparison to control SHAM rats. The realization of the ovariectomy or SHAM procedure occurred on Day 100 of life. All groups (n = 8) were followed-up for 180 days to address the study endpoints. Results: Micro-CT from androgenized female rats (SHAM) showed a divergence between the trabecular and cortical bone profiles. Compared to SHAM controls, these rats had an increase in trabecular bone mass with a diminution in bone resorption C-terminal telopeptide of type 1 collagen (CTX) (p < 0.05), a concomitant decrease in cortical area and thickness in the femur, and a reduction in the strength of the femur on the mechanical test (p < 0.01). Conclusions: Our results suggest that a reduction in the cortical thickness and cortical area observed in PCOS model rats was associated with a reduced strength of the femur, despite increased trabecular formation. Ovariectomy in the androgenized OVX group limited the progression rate of cortical bone loss, resulting in bone resistance and cortical thickness comparable to those observed in the control OVX group. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2023 The Authors. Published by Elsevier Inc.) |
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| فهرسة مساهمة: | Keywords: Animal models of PCOS; Bone; Mechanical test; Polycystic ovary syndrome; microCT |
| سلسلة جزيئية: | figshare 10.6084/m9.figshare.17088803 |
| تواريخ الأحداث: | Date Created: 20230828 Latest Revision: 20230829 |
| رمز التحديث: | 20230830 |
| مُعرف محوري في PubMed: | PMC10458295 |
| DOI: | 10.1016/j.bonr.2023.101710 |
| PMID: | 37637757 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2352-1872 |
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| DOI: | 10.1016/j.bonr.2023.101710 |