دورية أكاديمية
Quantitation of Circulating Mycobacterium tuberculosis Antigens by Nanopore Biosensing in Children Evaluated for Pulmonary Tuberculosis in South Africa.
| العنوان: | Quantitation of Circulating Mycobacterium tuberculosis Antigens by Nanopore Biosensing in Children Evaluated for Pulmonary Tuberculosis in South Africa. |
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| المؤلفون: | Wang X; Department of Chemical Engineering, University of South Carolina, Columbia, South Carolina 29208, United States., Wei X; Department of Chemical Engineering, University of South Carolina, Columbia, South Carolina 29208, United States.; Biomedical Engineering Program, University of South Carolina, Columbia, South Carolina 29208, United States., van der Zalm MM; Desmond Tutu TB Centre, Department of Paediatrics and Child Health, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, 8000, South Africa., Zhang Z; Biomedical Engineering Program, University of South Carolina, Columbia, South Carolina 29208, United States., Subramanian N; Biomedical Engineering Program, University of South Carolina, Columbia, South Carolina 29208, United States., Demers AM; Desmond Tutu TB Centre, Department of Paediatrics and Child Health, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, 8000, South Africa.; Division of Microbiology, Department of Laboratory Medicine, CHU Sainte-Justine, and Department of Microbiology, Immunology and Infectious Diseases, Faculty of Medicine, University of Montreal, Montreal, Quebec H3T 1C5, Canada., Ghimenton Walters E; Desmond Tutu TB Centre, Department of Paediatrics and Child Health, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, 8000, South Africa.; Newcastle upon Tyne Hospitals NHS Foundation Trust, Newcastle upon Tyne, NE1 4LP, United Kingdom., Hesseling A; Desmond Tutu TB Centre, Department of Paediatrics and Child Health, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, 8000, South Africa., Liu C; Department of Chemical Engineering, University of South Carolina, Columbia, South Carolina 29208, United States.; Biomedical Engineering Program, University of South Carolina, Columbia, South Carolina 29208, United States. |
| المصدر: | ACS nano [ACS Nano] 2023 Nov 14; Vol. 17 (21), pp. 21093-21104. Date of Electronic Publication: 2023 Aug 29. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101313589 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1936-086X (Electronic) Linking ISSN: 19360851 NLM ISO Abbreviation: ACS Nano Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington D.C. : American Chemical Society |
| مواضيع طبية MeSH: | Nanopores* , Tuberculosis, Pulmonary*/diagnosis , Mycobacterium tuberculosis* , Tuberculosis*/diagnosis, Humans ; Child ; South Africa ; Proteomics ; Sensitivity and Specificity |
| مستخلص: | Nanopore sensing of proteomic biomarkers lacks accuracy due to the ultralow abundance of targets, a wide variety of interferents in clinical samples, and the mismatch between pore and analyte sizes. By converting antigens to DNA probes via click chemistry and quantifying their characteristic signals, we show a nanopore assay with several amplification mechanisms to achieve an attomolar level limit of detection that enables quantitation of the circulating Mycobacterium tuberculosis ( Mtb ) antigen ESAT-6/CFP-10 complex in human serum. The assay's nonsputum-based feature and low-volume sample requirements make it particularly well-suited for detecting pediatric tuberculosis (TB) disease, where establishing an accurate diagnosis is greatly complicated by the paucibacillary nature of respiratory secretions, nonspecific symptoms, and challenges with sample collection. In the clinical assessment, the assay was applied to analyze ESAT-6/CFP-10 levels in serum samples collected during baseline investigation for TB in 75 children, aged 0-12 years, enrolled in a diagnostic study conducted in Cape Town, South Africa. This nanopore assay showed superior sensitivity in children with confirmed TB (94.4%) compared to clinical "gold standard" diagnostic technologies (Xpert MTB/RIF 44.4% and Mtb culture 72.2%) and filled the diagnostic gap for children with unconfirmed TB, where these traditional technologies fell short. We envision that, in combination with automated sample processing and portable nanopore devices, this methodology will offer a powerful tool to support the diagnosis of pulmonary TB in children. |
| References: | Tuberculosis (Edinb). 2013 Jul;93(4):381-8. (PMID: 23602700) Nat Nanotechnol. 2010 Feb;5(2):160-5. (PMID: 20023645) Lancet Glob Health. 2017 Sep;5(9):e898-e906. (PMID: 28807188) Nat Commun. 2017 Nov 16;8(1):1552. (PMID: 29146902) Nat Commun. 2021 Jun 7;12(1):3368. (PMID: 34099723) J Mater Chem B. 2020 Aug 12;8(31):6792-6797. (PMID: 32495805) J Electrochem Soc. 2022 May;169(5):. (PMID: 35599744) PLoS Med. 2011 Jul;8(7):e1001064. (PMID: 21814497) Nat Methods. 2019 Dec;16(12):1297-1305. (PMID: 31740818) Int J Tuberc Lung Dis. 2001 Jul;5(7):594-603. (PMID: 11467365) J Clin Microbiol. 2013 Feb;51(2):607-10. (PMID: 23175268) Anal Chem. 2020 Jul 7;92(13):9405-9411. (PMID: 32539349) Nat Biotechnol. 2014 Aug;32(8):829-33. (PMID: 24964173) J Clin Microbiol. 2005 May;43(5):2070-4. (PMID: 15872224) Clin Chem. 2018 May;64(5):791-800. (PMID: 29348166) Nat Commun. 2016 Jan 06;7:10217. (PMID: 26732171) PLoS One. 2011;6(12):e28815. (PMID: 22216117) BMC Med. 2021 May 18;19(1):113. (PMID: 34001096) PLoS Pathog. 2014 Oct 30;10(10):e1004446. (PMID: 25356553) Nat Commun. 2022 Nov 11;13(1):6852. (PMID: 36369146) Chem Sci. 2015 Oct 1;6(10):5628-5634. (PMID: 28757950) Sens Actuators B Chem. 2021 Jul 1;338:. (PMID: 33927481) BMC Infect Dis. 2013 Jan 23;13:31. (PMID: 23343418) Front Immunol. 2018 Nov 09;9:2476. (PMID: 30473692) Am J Respir Crit Care Med. 2000 Apr;161(4 Pt 1):1376-95. (PMID: 10764337) J Clin Microbiol. 2021 Jan 21;59(2):. (PMID: 33239373) ACS Sens. 2020 Jun 26;5(6):1707-1716. (PMID: 32403927) J Infect Dis. 2001 Jan 1;183(1):175-6. (PMID: 11106545) S Afr Med J. 2012 Sep 07;102(10):805-7. (PMID: 23034211) Nanotechnol Precis Eng. 2020 Mar;3(1):2-8. (PMID: 33786424) J Infect Dis. 2002 Dec 15;186(12):1797-807. (PMID: 12447766) FEBS J. 2006 Apr;273(7):1445-62. (PMID: 16689931) Nat Commun. 2019 May 8;10(1):2109. (PMID: 31068594) Brief Bioinform. 2021 Sep 2;22(5):. (PMID: 33483726) Sens Actuators Rep. 2023 Dec;6:. (PMID: 37663321) MMWR Recomm Rep. 2010 Jun 25;59(RR-5):1-25. (PMID: 20577159) Clin Infect Dis. 2015 Oct 15;61Suppl 3:S179-87. (PMID: 26409281) Nat Biotechnol. 2020 Feb;38(2):159-160. (PMID: 31974421) Nat Commun. 2017 Oct 16;8(1):935. (PMID: 29038539) Genomics Proteomics Bioinformatics. 2016 Oct;14(5):265-279. (PMID: 27646134) Biosens Bioelectron. 2020 Oct 1;165:112434. (PMID: 32729547) Nano Lett. 2013;13(12):6144-50. (PMID: 24171554) Clin Infect Dis. 2010 May 15;50 Suppl 3:S184-94. (PMID: 20397947) Nat Nanotechnol. 2009 Apr;4(4):265-70. (PMID: 19350039) Anal Chem. 2020 Feb 18;92(4):3042-3049. (PMID: 31970978) J Clin Microbiol. 2012 Dec;50(12):4188-9. (PMID: 23052304) Diagn Microbiol Infect Dis. 2015 Oct;83(2):162-4. (PMID: 26239846) Am J Respir Crit Care Med. 2009 Nov 1;180(9):903-8. (PMID: 19628775) Small. 2019 Jan;15(2):e1804078. (PMID: 30398696) Tuberculosis (Edinb). 2016 Dec;101S:S105-S108. (PMID: 27727131) J Clin Microbiol. 2011 Aug;49(8):3068-70. (PMID: 21677069) Small. 2018 Nov;14(46):e1802412. (PMID: 30225962) J Trop Pediatr. 2019 Apr 1;65(2):130-138. (PMID: 29846733) J Bacteriol. 2009 Sep;191(18):5865-7. (PMID: 19617365) Paediatr Respir Rev. 2007 Jun;8(2):107-17. (PMID: 17574154) Eur J Clin Microbiol Infect Dis. 2016 Jan;35(1):1-18. (PMID: 26476550) Sci Rep. 2021 Sep 28;11(1):19193. (PMID: 34584117) Small. 2013 Mar 11;9(5):750-9. (PMID: 23074081) Chem Commun (Camb). 2017 Oct 19;53(84):11564-11567. (PMID: 28990601) Proc Natl Acad Sci U S A. 2017 Apr 11;114(15):3969-3974. (PMID: 28348223) Nat Biotechnol. 2012 Apr 10;30(4):326-8. (PMID: 22491281) J Clin Lab Anal. 2015 Sep;29(5):375-82. (PMID: 25131423) Pediatr Infect Dis J. 2019 Jun;38(6):e128-e131. (PMID: 30418355) J Phys Chem B. 2013 May 2;117(17):4763-9. (PMID: 23565989) Biosens Bioelectron. 2021 Jun 1;181:113134. (PMID: 33761415) Nano Today. 2022 Aug;45:. (PMID: 37034182) Expert Rev Gastroenterol Hepatol. 2022 Jun;16(6):577-586. (PMID: 35678762) Arch Dis Child. 2005 Nov;90(11):1162-5. (PMID: 16131501) J Clin Microbiol. 2011 Mar;49(3):902-7. (PMID: 21159936) Nat Commun. 2019 Jul 31;10(1):3359. (PMID: 31366910) |
| معلومات مُعتمدة: | K22 AI136686 United States AI NIAID NIH HHS; K43 TW011028 United States TW FIC NIH HHS; R61 AI174295 United States AI NIAID NIH HHS |
| فهرسة مساهمة: | Keywords: CFP-10; ESAT-6; blood test; nanopore; pediatric tuberculosis |
| المشرفين على المادة: | 144058-44-6 (Mycobacterium tuberculosis antigens) |
| تواريخ الأحداث: | Date Created: 20230829 Date Completed: 20231116 Latest Revision: 20231125 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC10668583 |
| DOI: | 10.1021/acsnano.3c04420 |
| PMID: | 37643288 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1936-086X |
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| DOI: | 10.1021/acsnano.3c04420 |