دورية أكاديمية
MiR-5189-3p Suppresses cell Proliferation, Invasion and Migration Through Targeting EIF5A2 in Laryngeal Squamous Cell Carcinoma.
| العنوان: | MiR-5189-3p Suppresses cell Proliferation, Invasion and Migration Through Targeting EIF5A2 in Laryngeal Squamous Cell Carcinoma. |
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| المؤلفون: | Lou D; Department of Otolaryngology, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.; Department of Otolaryngology, The First Hospital of Qinhuangdao, Qinhuangdao, 066000, Hebei, China., Jia Q; Department of Otolaryngology, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China., Zhang H; Department of Otolaryngology, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China., Wang J; Department of Otolaryngology, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China., Liu L; Department of Otolaryngology, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China., Liu Z; Department of Otolaryngology, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China., Jia X; Department of Otolaryngology, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China., Wang J; Department of Otolaryngology, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China. jianxingwang2008@163.com., Shan C; Department of Otolaryngology, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China. shanchunguang0825@hb2h.com. |
| المصدر: | Biochemical genetics [Biochem Genet] 2024 Jun; Vol. 62 (3), pp. 1603-1616. Date of Electronic Publication: 2023 Sep 01. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Kluwer Academic/Plenum Publishers Country of Publication: United States NLM ID: 0126611 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4927 (Electronic) Linking ISSN: 00062928 NLM ISO Abbreviation: Biochem Genet Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1999- : New York : Kluwer Academic/Plenum Publishers Original Publication: New York, Plenum Press. |
| مواضيع طبية MeSH: | MicroRNAs*/genetics , MicroRNAs*/metabolism , Cell Proliferation* , Cell Movement* , Peptide Initiation Factors*/genetics , Peptide Initiation Factors*/metabolism , Laryngeal Neoplasms*/genetics , Laryngeal Neoplasms*/pathology , Laryngeal Neoplasms*/metabolism , Eukaryotic Translation Initiation Factor 5A* , RNA-Binding Proteins*/genetics , RNA-Binding Proteins*/metabolism , Neoplasm Invasiveness*, Humans ; Cell Line, Tumor ; Gene Expression Regulation, Neoplastic ; Carcinoma, Squamous Cell/genetics ; Carcinoma, Squamous Cell/pathology ; Carcinoma, Squamous Cell/metabolism ; Squamous Cell Carcinoma of Head and Neck/genetics ; Squamous Cell Carcinoma of Head and Neck/metabolism ; Squamous Cell Carcinoma of Head and Neck/pathology |
| مستخلص: | A growing body of evidence suggests that miR-5189-3p plays a critical role in multiple diseases. This study aimed to investigate the function of miR-5189-3p in laryngeal squamous cell carcinoma (LSCC) and explore its underlying mechanisms. qRT-PCR was designed to determine the expression levels of miR-5189-3p and eukaryotic translation initiation factor 5A2 (EIF5A2), while CCK-8 assay was performed to measure the effects of miR-5189-3p on cell proliferation. Transwell assay was performed to evaluate cell invasion as well as migration, and wound healing assay was applied to demonstrate cell migratory ability. Target gene prediction and luciferase reporter assay were developed to screen the possible target gene of miR-5189-3p, and Western blot was designed to measure EIF5A2 protein expression. MiR-5189-3p was down-regulated in LSCC tissues and cell lines. Up-regulation of miR-5189-3p notably inhibited cell proliferation, invasion, and migration in HEP2 and FADU cells. EIF5A2 was the potential downstream gene of miR-5189-3p, and overexpression of miR-5189-3p apparently reduced EIF5A2 expression. Moreover, reintroduction of EIF5A2 rescued the tumor suppressive effects of miR-5189-3p. MiR-5189-3p functions as a tumor inhibitor in LSCC progression via directly regulating EIF5A2 and may be a potential therapeutic target for LSCC. (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.) |
| References: | Bao Y, Zhang Y, Lu Y, Guo H, Dong Z, Chen Q, Zhang X, Shen W, Chen W, Wang X (2020) Overexpression of microRNA-9 enhances cisplatin sensitivity in hepatocellular carcinoma by regulating EIF5A2-mediated epithelial-mesenchymal transition. Int J Biol Sci 16(5):827–837. https://doi.org/. (PMID: 10.7150/ijbs.32460320715527019138) Janiszewska J, Szaumkessel M, Szyfter K (2013) microRNAs are important players in head and neck carcinoma: a review. Crit Rev Oncol/Hematol 88(3):716–728. https://doi.org/. (PMID: 10.1016/j.critrevonc.2013.07.01223948550) Johnson DE, Burtness B, Leemans CR, Lui VWY, Bauman JE, Grandis JR (2020) Head and neck squamous cell carcinoma. Nat Rev Dis Primers 6(1):92. https://doi.org/. (PMID: 10.1038/s41572-020-00224-3332439867944998) Jung K, Narwal M, Min SY, Keam B, Kang H (2020) Squamous cell carcinoma of head and neck: what internists should know. Korean J Intern Med 35(5):1031–1044. https://doi.org/. (PMID: 10.3904/kjim.2020.078326639137487309) Koshizuka K, Hanazawa T, Arai T, Okato A, Kikkawa N, Seki N (2017) Involvement of aberrantly expressed microRNAs in the pathogenesis of head and neck squamous cell carcinoma. Cancer Metastasis Rev 36(3):525–545. https://doi.org/. (PMID: 10.1007/s10555-017-9692-y28836104) Lin S, Gregory RI (2015) MicroRNA biogenesis pathways in cancer. Nat Reviews: Cancer 15(6):321–333. https://doi.org/. (PMID: 10.1038/nrc393225998712) Lu E, Su J, Zeng W, Zhang C (2016) Enhanced miR-9 promotes laryngocarcinoma cell survival via down-regulating PTEN. Biomed Pharmacotherapy 84:608–613. https://doi.org/. (PMID: 10.1016/j.biopha.2016.09.047) Lu J, Fang Q, Ge X (2021) Role and mechanism of mir-5189-3p in deep vein thrombosis of Lower Extremities. Ann Vasc Surg 77:288–295. https://doi.org/. (PMID: 10.1016/j.avsg.2021.07.00434416282) Mathews MB, Hershey JW (2015) The translation factor eIF5A and human cancer. Biochim Biophys Acta 1849(7):836–844. https://doi.org/. (PMID: 10.1016/j.bbagrm.2015.05.002259798264732523) Ning L, Wang L, Zhang H, Jiao X, Chen D (2020) Eukaryotic translation initiation factor 5A in the pathogenesis of cancers. Oncol Lett 20(4):81. https://doi.org/. (PMID: 10.3892/ol.2020.11942328639147436936) Pan Q, Zhao J, Li M, Liu X, Xu Y, Li W, Wu S, Su Z (2020) Exosomal miRNAs are potential diagnostic biomarkers between malignant and benign thyroid nodules based on next-generation sequencing. Carcinogenesis 41(1):18–24. https://doi.org/. (PMID: 31560760) Salinas-Vera YM, Valdes J, Hidalgo-Miranda A, Cisneros-Villanueva M, Marchat LA, Nunez-Olvera SI, Ramos-Payan R, Perez-Plasencia C, Arriaga-Pizano LA, Prieto-Chavez JL, Lopez-Camarillo C (2022) Three-dimensional organotypic cultures reshape the microRNAs Transcriptional program in breast Cancer cells. Cancers 14(10). https://doi.org/. Smolarz B, Durczynski A, Romanowicz H, Szyllo K, Hogendorf P (2022) miRNAs in Cancer (Review of Literature). Int J Mol Sci 23(5). https://doi.org/. Takeuchi T, Kawasaki H, Luce A, Cossu AM, Misso G, Scrima M, Bocchetti M, Ricciardiello F, Caraglia M, Zappavigna S (2020) Insight toward the MicroRNA profiling of laryngeal cancers: Biological Role and Clinical Impact. Int J Mol Sci 21(10). https://doi.org/. Tian SB, Yu JC, Liu YQ, Kang WM, Ma ZQ, Ye X, Yan C (2015) MiR-30b suppresses tumor migration and invasion by targeting EIF5A2 in gastric cancer. World J Gastroenterol 21(31):9337–9347. https://doi.org/. (PMID: 10.3748/wjg.v21.i31.9337263093594541385) Wang FW, Guan XY, Xie D (2013) Roles of eukaryotic initiation factor 5A2 in human cancer. Int J Biol Sci 9(10):1013–1020. https://doi.org/. (PMID: 10.7150/ijbs.7191242502463831114) Wu X, Cui CL, Chen WL, Fu ZY, Cui XY, Gong X (2016) miR-144 suppresses the growth and metastasis of laryngeal squamous cell carcinoma by targeting IRS1. Am J Transl Res 8(1):1–11. (PMID: 270695354759411) Wu GQ, Xu YM, Lau ATY (2020) Recent insights into eukaryotic translation initiation factors 5A1 and 5A2 and their roles in human health and disease. Cancer Cell Int 20:142. https://doi.org/. (PMID: 10.1186/s12935-020-01226-7323681887191727) Xu L, Chen Z, Xue F, Chen W, Ma R, Cheng S, Cui P (2015) MicroRNA-24 inhibits growth, induces apoptosis, and reverses radioresistance in laryngeal squamous cell carcinoma by targeting X-linked inhibitor of apoptosis protein. Cancer Cell Int 15:61. https://doi.org/. (PMID: 10.1186/s12935-015-0217-x261062834477309) Yang Y, Cui H, Wang X (2019) Downregulation of EIF5A2 by mir-221-3p inhibits cell proliferation, promotes cell cycle arrest and apoptosis in medulloblastoma cells. Biosci Biotechnol Biochem 83(3):400–408. https://doi.org/. (PMID: 10.1080/09168451.2018.155360430551723) Yu X, Li Z (2015) The role of microRNAs expression in laryngeal cancer. Oncotarget 6(27):23297–23305. https://doi.org/. (PMID: 10.18632/oncotarget.4195260796424695119) Zhang Y, Tu B, Sha Q, Qian J (2022) Bone marrow mesenchymal stem cells-derived exosomes suppress miRNA-5189-3p to increase fibroblast-like synoviocyte apoptosis via the BATF2/JAK2/STAT3 signaling pathway. Bioengineered 13(3):6767–6780. https://doi.org/. (PMID: 10.1080/21655979.2022.2045844352460068973596) Zhao G, Zhang W, Dong P, Watari H, Guo Y, Pfeffer LM, Tigyi G, Yue J (2021) EIF5A2 controls ovarian tumor growth and metastasis by promoting epithelial to mesenchymal transition via the TGFbeta pathway. Cell & Bioscience 11(1):70. https://doi.org/. (PMID: 10.1186/s13578-021-00578-5) |
| معلومات مُعتمدة: | 20372401D Hebei Province Development of Bureau Science and Technology |
| فهرسة مساهمة: | Keywords: EIF5A2; Laryngeal carcinoma; Target; miR-5189-3p |
| المشرفين على المادة: | 0 (MicroRNAs) 0 (Peptide Initiation Factors) 0 (Eukaryotic Translation Initiation Factor 5A) 0 (RNA-Binding Proteins) 0 (eIF-5A2) |
| تواريخ الأحداث: | Date Created: 20230901 Date Completed: 20240619 Latest Revision: 20240619 |
| رمز التحديث: | 20240620 |
| DOI: | 10.1007/s10528-023-10489-4 |
| PMID: | 37656330 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1573-4927 |
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| DOI: | 10.1007/s10528-023-10489-4 |