دورية أكاديمية

Involvement of phospholipase A 2 in the neuromuscular blockade caused by coralsnake (Micrurus spp.) venoms in mouse phrenic nerve-diaphragm preparations in vitro.

التفاصيل البيبلوغرافية
العنوان: Involvement of phospholipase A 2 in the neuromuscular blockade caused by coralsnake (Micrurus spp.) venoms in mouse phrenic nerve-diaphragm preparations in vitro.
المؤلفون: Couceiro FYGM; Laboratory of Toxinology and Cardiovascular Research, Graduate Program in Health Sciences, University of Western São Paulo (UNOESTE), Presidente Prudente, SP, Brazil., Demico PJ; Laboratory of Toxinology and Cardiovascular Research, Graduate Program in Health Sciences, University of Western São Paulo (UNOESTE), Presidente Prudente, SP, Brazil., Dias SR; Laboratory of Toxinology and Cardiovascular Research, Graduate Program in Health Sciences, University of Western São Paulo (UNOESTE), Presidente Prudente, SP, Brazil., Oliveira IN; Laboratory of Toxinology and Cardiovascular Research, Graduate Program in Health Sciences, University of Western São Paulo (UNOESTE), Presidente Prudente, SP, Brazil., Pacagnelli FL; Laboratory of Toxinology and Cardiovascular Research, Graduate Program in Health Sciences, University of Western São Paulo (UNOESTE), Presidente Prudente, SP, Brazil., Silva EO; Laboratory of Pathological Anatomy, Veterinary Hospital, University of Western São Paulo (UNOESTE), Presidente Prudente, SP, Brazil., Sant'Anna SS; Laboratory of Herpetology, Butantan Institute (IB), São Paulo, SP, Brazil., Grego KF; Laboratory of Herpetology, Butantan Institute (IB), São Paulo, SP, Brazil., Morais-Zani K; Laboratory of Herpetology, Butantan Institute (IB), São Paulo, SP, Brazil., Torres-Bonilla KA; Section of Pharmacology, Department of Translational Medicine, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, SP, Brazil., Hyslop S; Section of Pharmacology, Department of Translational Medicine, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, SP, Brazil., Floriano RS; Laboratory of Toxinology and Cardiovascular Research, Graduate Program in Health Sciences, University of Western São Paulo (UNOESTE), Presidente Prudente, SP, Brazil. Electronic address: rafael@unoeste.br.
المصدر: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2023 Oct; Vol. 234, pp. 107263. Date of Electronic Publication: 2023 Sep 01.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Pergamon Press Country of Publication: England NLM ID: 1307333 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3150 (Electronic) Linking ISSN: 00410101 NLM ISO Abbreviation: Toxicon Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford ; New York : Pergamon Press, c1962-
مستخلص: In this work, we examined the neuromuscular blockade caused by venoms from four South-American coralsnakes (Micrurus altirostris - MA, M. corallinus - MC, M. spixii - MS, and M. dumerilii carinicauda - MDC) and the ability of varespladib (VPL), a phospholipase A 2 (PLA 2 ) inhibitor, to attenuate this blockade. PLA 2 activity was determined using a colorimetric assay and a fixed amount of venom (10 μg). Neurotoxicity was assayed using a single concentration of venom (10 μg/ml) in mouse phrenic nerve-diaphragm (PND) preparations mounted for myographic recordings and then subjected to histological analysis. All venoms showed PLA 2 activity, with MS and MA venoms having the highest (15.53 ± 1.9 A 425 nm /min) and lowest (0.23 ± 0.14 A 425 nm /min) activities, respectively. VPL (292 and 438 μM) inhibited the PLA 2 activity of all venoms, although that of MA venom was least affected. All venoms caused neuromuscular blockade, with MS and MDC venoms causing the fastest and slowest 100% blockade [in 40 ± 3 min and 120 ± 6 min (n = 4), respectively]; MA and MC produced complete blockade within 90-100 min. Preincubation of venoms with 292 μM VPL attenuated the blockade to varying degrees: the greatest inhibition was seen with MDC venom and blockade by MS venom was unaffected by this inhibitor. These results indicate that PLA 2 has a variable contribution to coralsnake venom-induced neuromuscular blockade in vitro, with the insensitivity of MS venom to VPL suggesting that blockade by this venom is mediated predominantly by post-synaptically-active α-neurotoxins.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Coralsnake venom; Neuromuscular blockade; Peripheral neurotoxicity; Phospholipase A(2); Varespladib
تواريخ الأحداث: Date Created: 20230902 Latest Revision: 20231014
رمز التحديث: 20231215
DOI: 10.1016/j.toxicon.2023.107263
PMID: 37659667
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-3150
DOI:10.1016/j.toxicon.2023.107263