دورية أكاديمية
Gastroprotective potential of red onion (Allium cepa L.) peel in ethanol-induced gastric injury in rats: Involvement of Nrf2/HO-1 and HMGB-1/NF-κB trajectories.
| العنوان: | Gastroprotective potential of red onion (Allium cepa L.) peel in ethanol-induced gastric injury in rats: Involvement of Nrf2/HO-1 and HMGB-1/NF-κB trajectories. |
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| المؤلفون: | Ali NB; Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt., Abdelhamid Ibrahim SS; Department of Pharmacology and Therapeutics, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt., Alsherbiny MA; Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt; Innovation Centre, Victor Chang Cardiac Research Institute, Darlinghurst, Sydney, Australia; NICM Health Research Institute, Western Sydney University, Penrith, NSW, 2747, Australia., Sheta E; Department of Pathology, Faculty of Medicine, Alexandria University, Alexandria, Egypt., El-Shiekh RA; Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt. Electronic address: riham.adel@pharma.cu.edu.eg., Ashour RM; Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt., El-Gazar AA; Department of Pharmacology and Toxicology, Faculty of Pharmacy, October 6 University, 12585, Egypt., Ragab GM; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Misr University for Science and Technology, 12585, Egypt., El-Gayed SH; Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt; Department of Pharmacognosy, Faculty of Pharmacy, 6th October University, Cairo, Egypt., Li CG; NICM Health Research Institute, Western Sydney University, Penrith, NSW, 2747, Australia., Abdel-Sattar E; Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt. Electronic address: essam.abdelsattar@pharma.cu.edu.eg. |
| المصدر: | Journal of ethnopharmacology [J Ethnopharmacol] 2024 Jan 30; Vol. 319 (Pt 1), pp. 117115. Date of Electronic Publication: 2023 Sep 01. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Sequoia Country of Publication: Ireland NLM ID: 7903310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7573 (Electronic) Linking ISSN: 03788741 NLM ISO Abbreviation: J Ethnopharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : Elsevier Sequoia Original Publication: Lausanne, Elsevier Sequoia. |
| مواضيع طبية MeSH: | Stomach Ulcer*/chemically induced , Stomach Ulcer*/drug therapy , Stomach Ulcer*/pathology , Anti-Ulcer Agents*/pharmacology , Anti-Ulcer Agents*/therapeutic use , Anti-Ulcer Agents*/chemistry, Rats ; Animals ; Ethanol/therapeutic use ; Onions ; NF-kappa B/metabolism ; Ulcer/drug therapy ; Anthocyanins ; NF-E2-Related Factor 2/metabolism ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Plant Extracts/chemistry ; Flavonoids/therapeutic use ; Phytochemicals/adverse effects ; HMGB Proteins/metabolism ; Gastric Mucosa/pathology |
| مستخلص: | Ethnopharmacological Relevance: The utilization of plants with therapeutic properties in traditional medicine has a longstanding practice. Among them, the well-known Allium cepa L. commonly known as onion has been valued for its anti-inflammatory and antioxidant potential in the treatment of various ailments, including gastric ulcers. Aim of the Study: This study investigated the gastroprotective potential of red onion peel extract and its fractions in a rat model of ethanol-induced gastric ulcer. Moreover, their phytochemical profiles were compared to identify the active metabolites. Materials and Methods: Mass spectrometry-based metabolomics and chemometrics were performed for phytochemical analysis. Ethanol-induced gastric ulcer model was used to assess the gastroprotective activity. Nine groups of rats were allocated as follows: Group 1 was the normal control; Group 2 rats were used as a positive control/model and received 1 mL of absolute ethanol; and Group 3 rats were treated with famotidine at a dose of 20 mg/kg orally. Group 4 and 5 rats were treated with total acidified ethanolic extract (T1, T2). Group 6 and 7 rats were treated with anthocyanins-rich fractions (P1, P2). Groups 8 and 9 were the flavonoids-rich fraction (S1, S2) treatment. Prior to scarification, the ulcer index in mm was obtained from gastric tissues photographed beside a ruler with further analysis using ImageJ software. Results: Seventy key major and discriminatory metabolites were identified including flavonoids, anthocyanins, phenolic acids, and miscellaneous compounds. The examined extract and its fractions significantly reduced the ulcer index and inflammatory cytokines via downregulating HMGB-1/NF-κB. Also, they augmented the expression of Nrf2/HO-1 and reduced NOX1/4 mRNA expression. Moreover, there was a significant reduction in the oxidative stress and apoptotic biomarkers as well as a noticeable enhancement in histopathological changes of the stomach tissues. Conclusion: Red onion peels have a promising dose dependent gastroprotective potential in alcohol-induced ulcers via modulating Nrf2/HO-1 and HMGB-1/NF-κB trajectories. This highlights the potential of red onion peels in treating gastric ulcers. Competing Interests: Declaration of competing interest We wish to confirm that there are no known conflicts of interest associated with this publication of “Gastroprotective Potential of Red Onion (Allium cepa L.) Peel in Ethanol-Induced Gastric Injury in Rats: Involvement of Nrf2/HO-1 and HMGB-1/NF-κB Trajectories” to be published in Journal of Ethnopharmacology. With the submission of this manuscript I would like to undertake that the above mentioned manuscript has not been published elsewhere, accepted for publication elsewhere or under editorial review for publication elsewhere; and that my Institute's (Faculty of Pharmacy, Cairo University) representative is fully aware of this submission. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Ethanol-induced gastric ulcer rat model; Metabolomics; Nrf2/HO-1 and HMGB-1/NF-κB; Red onion peel (Allium cepa L.); UPLC-qTOF-MS |
| المشرفين على المادة: | 3K9958V90M (Ethanol) 0 (NF-kappa B) 0 (Anthocyanins) 0 (NF-E2-Related Factor 2) 0 (Plant Extracts) 0 (Anti-Ulcer Agents) 0 (Flavonoids) 0 (Phytochemicals) 0 (HMGB Proteins) |
| تواريخ الأحداث: | Date Created: 20230902 Date Completed: 20231122 Latest Revision: 20231122 |
| رمز التحديث: | 20231122 |
| DOI: | 10.1016/j.jep.2023.117115 |
| PMID: | 37659760 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7573 |
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| DOI: | 10.1016/j.jep.2023.117115 |