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Semiconducting Polymer Nanospherical Nucleic Acid Probe for Transcriptomic Imaging of Cancer Chemo-Immunotherapy.

التفاصيل البيبلوغرافية
العنوان: Semiconducting Polymer Nanospherical Nucleic Acid Probe for Transcriptomic Imaging of Cancer Chemo-Immunotherapy.
المؤلفون: Xu C; School of Chemistry Chemical Engineering and Biotechnology, Nanyang Technological University, 70 Nanyang Drive, Singapore, 637457, Singapore., Yu J; School of Chemistry Chemical Engineering and Biotechnology, Nanyang Technological University, 70 Nanyang Drive, Singapore, 637457, Singapore., Ning X; School of Chemistry Chemical Engineering and Biotechnology, Nanyang Technological University, 70 Nanyang Drive, Singapore, 637457, Singapore., Xu M; School of Chemistry Chemical Engineering and Biotechnology, Nanyang Technological University, 70 Nanyang Drive, Singapore, 637457, Singapore., He S; School of Chemistry Chemical Engineering and Biotechnology, Nanyang Technological University, 70 Nanyang Drive, Singapore, 637457, Singapore., Wu J; School of Chemistry Chemical Engineering and Biotechnology, Nanyang Technological University, 70 Nanyang Drive, Singapore, 637457, Singapore., Pu K; School of Chemistry Chemical Engineering and Biotechnology, Nanyang Technological University, 70 Nanyang Drive, Singapore, 637457, Singapore.; Lee Kong Chian School of Medicine, Nanyang Technological University, 59 Nanyang Drive, Singapore, 636921, Singapore.
المصدر: Advanced materials (Deerfield Beach, Fla.) [Adv Mater] 2023 Nov; Vol. 35 (48), pp. e2306739. Date of Electronic Publication: 2023 Oct 24.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 9885358 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-4095 (Electronic) Linking ISSN: 09359648 NLM ISO Abbreviation: Adv Mater Subsets: MEDLINE
أسماء مطبوعة: Publication: Sept. 3, 1997- : Weinheim : Wiley-VCH
Original Publication: Deerfield Beach, FL : VCH Publishers, 1989-
مواضيع طبية MeSH: Neoplasms*/diagnostic imaging , Neoplasms*/drug therapy , Nanoparticles*, Animals ; Mice ; Polymers ; Transcriptome ; Nucleic Acid Probes ; RNA ; Optical Imaging/methods ; DNA ; Immunotherapy
مستخلص: Real-time in vivo imaging of RNA can enhance the understanding of physio-pathological processes. However, most nucleic acid-based sensors have poor resistance to nucleases and limited photophysical properties, making them suboptimal for this purpose. To address this, a semiconducting polymer nanospherical nucleic acid probe (SENSE) for transcriptomic imaging of cancer immunity in living mice is developed. SENSE comprises a semiconducting polymer (SP) backbone covalently linked with recognition DNA strands, which are complemented by dye-labeled signal DNA strands. Upon detection of targeted T lymphocyte transcript (Gzmb: granzyme B), the signal strands are released, leading to a fluorescence enhancement correlated to transcript levels with superb sensitivity. The always-on fluorescence of the SP core also serves as an internal reference for tracking SENSE uptake in tumors. Thus, SENSE has the dual-signal channel that enables ratiometric imaging of Gzmb transcripts in the tumor of living mice for evaluating chemo-immunotherapy; moreover, it has demonstrated sensitivity and specificity comparable to flow cytometry and quantitative polymerase chain reaction,  yet offering a faster and simpler means of T cell detection in resected tumors. Therefore, SENSE represents a promising tool for in vivo RNA imaging.
(© 2023 Wiley-VCH GmbH.)
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معلومات مُعتمدة: RG125/19 Singapore Ministry of Education; RT05/20 Singapore Ministry of Education; MOE2018-T2-2-042 Singapore Ministry of Education; MOE-T2EP30220-0010 Singapore Ministry of Education; NRF-NRFI07-2021-0005 Singapore National Research Foundation
فهرسة مساهمة: Keywords: RNA detection; cancer immunotherapy; fluorescence imaging; semiconducting polymer nanoparticles
المشرفين على المادة: 0 (Polymers)
0 (Nucleic Acid Probes)
63231-63-0 (RNA)
9007-49-2 (DNA)
تواريخ الأحداث: Date Created: 20230903 Date Completed: 20231129 Latest Revision: 20231129
رمز التحديث: 20240628
DOI: 10.1002/adma.202306739
PMID: 37660291
قاعدة البيانات: MEDLINE
الوصف
تدمد:1521-4095
DOI:10.1002/adma.202306739