دورية أكاديمية
Single-cell transcriptomics supports presence of cryptic species and reveals low levels of population genetic diversity in two testate amoebae morphospecies with large population sizes.
| العنوان: | Single-cell transcriptomics supports presence of cryptic species and reveals low levels of population genetic diversity in two testate amoebae morphospecies with large population sizes. |
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| المؤلفون: | Weiner AKM; Department of Biological Sciences, Smith College, Northampton, MA, United States.; NORCE Climate and Environment, NORCE Norwegian Research Centre AS, Bergen, Norway., Sehein T; Department of Biological Sciences, Smith College, Northampton, MA, United States., Cote-L'Heureux A; Department of Biological Sciences, Smith College, Northampton, MA, United States., Sleith RS; Department of Biological Sciences, Smith College, Northampton, MA, United States.; Bigelow Laboratory for Ocean Sciences, East Boothbay, ME, United States., Greco M; Department of Biological Sciences, Smith College, Northampton, MA, United States., Malekshahi C; Department of Biological Sciences, Smith College, Northampton, MA, United States., Ryan-Embry C; Department of Biological Sciences, Smith College, Northampton, MA, United States., Ostriker N; Department of Biological Sciences, Smith College, Northampton, MA, United States., Katz LA; Department of Biological Sciences, Smith College, Northampton, MA, United States.; University of Massachusetts Amherst, Program in Organismic and Evolutionary Biology, Amherst, MA, United States. |
| المصدر: | Evolution; international journal of organic evolution [Evolution] 2023 Nov 02; Vol. 77 (11), pp. 2472-2483. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0373224 Publication Model: Print Cited Medium: Internet ISSN: 1558-5646 (Electronic) Linking ISSN: 00143820 NLM ISO Abbreviation: Evolution Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : Oxford : Oxford University Press Original Publication: Lancaster, Pa. : Society for the Study of Evolution |
| مواضيع طبية MeSH: | Amoeba*/genetics, Population Density ; Transcriptome ; Phylogeny ; Genetics, Population |
| مستخلص: | The enormous population sizes and wide biogeographical distribution of many microbial eukaryotes set the expectation of high levels of intraspecific genetic variation. However, studies investigating protist populations remain scarce, mostly due to limited 'omics data. Instead, most genetics studies of microeukaryotes have thus far relied on single loci, which can be misleading and do not easily allow for detection of recombination, a hallmark of sexual reproduction. Here, we analyze >40 genes from 72 single-cell transcriptomes from two morphospecies-Hyalosphenia papilio and Hyalosphenia elegans-of testate amoebae (Arcellinida, Amoebozoa) to assess genetic diversity in samples collected over four years from New England bogs. We confirm the existence of cryptic species based on our multilocus dataset, which provides evidence of recombination within and high levels of divergence between the cryptic species. At the same time, total levels of genetic diversity within cryptic species are low, suggesting that these abundant organisms have small effective population sizes, perhaps due to extinction and repopulation events coupled with efficient modes of dispersal. This study is one of the first to investigate population genetics in uncultivable heterotrophic protists using transcriptomics data and contributes towards understanding cryptic species of nonmodel microeukaryotes. (© The Author(s) 2023. Published by Oxford University Press on behalf of The Society for the Study of Evolution (SSE). All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
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| معلومات مُعتمدة: | United States NH NIH HHS; R15 HG010409 United States HG NHGRI NIH HHS |
| فهرسة مساهمة: | Keywords: Arcellinida; ploidy; population genetics; protists; recombination; transcriptomics |
| تواريخ الأحداث: | Date Created: 20230906 Date Completed: 20231109 Latest Revision: 20231113 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC10629589 |
| DOI: | 10.1093/evolut/qpad158 |
| PMID: | 37672006 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1558-5646 |
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| DOI: | 10.1093/evolut/qpad158 |