دورية أكاديمية
Abomasal RNA-seq reveals a strong local cellular response in suckling lambs with resistance against Haemonchus contortus.
العنوان: | Abomasal RNA-seq reveals a strong local cellular response in suckling lambs with resistance against Haemonchus contortus. |
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المؤلفون: | Lins JGG; São Paulo State University (UNESP), School of Veterinary Medicine and Animal Science, Botucatu, SP, Brazil; São Paulo State University (UNESP), Institute of Biosciences, Botucatu, SP, Brazil. Electronic address: jose.lins@unesp.br., Albuquerque ACA; São Paulo State University (UNESP), Institute of Biosciences, Botucatu, SP, Brazil., Almeida FA; São Paulo State University (UNESP), Institute of Biosciences, Botucatu, SP, Brazil., Britton C; Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom., Malossi C; São Paulo State University (UNESP), Institute of Biosciences, Botucatu, SP, Brazil., Araújo-Júnior JP; São Paulo State University (UNESP), Institute of Biosciences, Botucatu, SP, Brazil., Louvandini H; Center for Nuclear Energy in Agriculture, University of São Paulo, Piracicaba, SP, Brazil., Amarante AFT; São Paulo State University (UNESP), Institute of Biosciences, Botucatu, SP, Brazil. |
المصدر: | International journal for parasitology [Int J Parasitol] 2023 Nov; Vol. 53 (13), pp. 739-749. Date of Electronic Publication: 2023 Sep 04. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Science Country of Publication: England NLM ID: 0314024 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0135 (Electronic) Linking ISSN: 00207519 NLM ISO Abbreviation: Int J Parasitol Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Oxford : Elsevier Science Original Publication: Oxford, New York, Pergamon Press. |
مواضيع طبية MeSH: | Haemonchus*/genetics , Sheep Diseases*/genetics , Haemonchiasis*/genetics , Haemonchiasis*/veterinary, Sheep ; Animals ; RNA-Seq ; Immunity, Innate ; Adaptive Immunity ; Disease Susceptibility ; Feces ; Parasite Egg Count/veterinary |
مستخلص: | Santa Ines (SI) and Ile de France (IF) sheep are known to be resistant and susceptible to Haemonchus contortus infection, respectively. Several studies have shown some genes as potential biological markers for sheep resistance against gastrointestinal nematodes using molecular tools, including transcriptomic analysis. In this study, we sequenced the polyadenylated RNA of the abomasal tissue of SI and IF suckling lambs to identify mucosa-specific transcript alterations between breeds artificially infected with H. contortus. Naïve SI (n = 4) and IF (n = 4) lambs were artificially infected every other day, over a period of 52 days, from 14 to 66 days old, with a total of 5,400 H. contortus infective larvae. Fundic abomasal tissue samples were collected at 68 days old, and submitted to high-throughput RNA sequencing (RNA-seq). Differential expression analysis (P value < 0.001 and False Discovery Rate (FDR) < 0.05) between SI and IF samples identified 292 genes, most of which showed greater expression in SI lambs. To help annotate and assign possible function to differentially expressed genes (DEGs), we used previously available single-cell RNA-seq (scRNA-seq) data from ovine abomasal mucosa to putatively identify cell types and possible mechanisms involved in resistance to H. contortus. In particular, genes associated with endothelial and tuft cells showed the greatest increases in expression in SI relative to IF lambs. SI lambs had higher percentages of tuft cells than IF lambs in the fundic abomasal mucosa. Although we found innate immunity (cell-mediated in mucosa) acting as a protagonist in impairing H. contortus infection, a stronger acquired immune response was being modulated at an earlier stage by SI lambs. We suggest that the complex connection between innate and adaptive immunity is via cellular antigen processing and presentation (APP). Based on comparison with scRNA-seq data, SI lambs showed a robust APP mechanism characterized mainly by greater T cell APP, macrophage differentiation, and cytokine signalling. We identified potential mechanisms and markers to advance knowledge for selection of H. contortus resistance at a very early age, in SI as well as in other commercial sheep breeds. (Copyright © 2023 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.) |
فهرسة مساهمة: | Keywords: Breed resistance; Gene expression; Immune response; Nematode; Transcriptome |
تواريخ الأحداث: | Date Created: 20230906 Date Completed: 20231113 Latest Revision: 20231113 |
رمز التحديث: | 20240628 |
DOI: | 10.1016/j.ijpara.2023.06.008 |
PMID: | 37673202 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1879-0135 |
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DOI: | 10.1016/j.ijpara.2023.06.008 |