دورية أكاديمية
Surveillance of Vermont wildlife in 2021-2022 reveals no detected SARS-CoV-2 viral RNA.
| العنوان: | Surveillance of Vermont wildlife in 2021-2022 reveals no detected SARS-CoV-2 viral RNA. |
|---|---|
| المؤلفون: | Despres HW; Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, VT, 05405, USA., Mills MG; Virology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, USA., Schmidt MM; Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, VT, 05405, USA., Gov J; Virology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, USA., Perez Y; Virology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, USA., Jindrich M; Virology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, USA., Crawford AML; Virology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, USA., Kohl WT; Virology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, USA., Rosenblatt E; Rubenstein School of Environment and Natural Resources, University of Vermont, 81 Carrigan Dr, Burlington, VT, 05405, USA., Kubinski HC; Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, VT, 05405, USA., Simmons BC; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Nippes MC; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Goldenberg AJ; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Murtha KE; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Nicoloro S; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Harris MJ; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Feeley AC; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Gelinas TK; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Cronin MK; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Frederick RS; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Thomas M; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Johnson ME; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Murphy J; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Lenzini EB; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Carr PA Jr; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Berger DH; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Mehta SP; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Floreani CJ; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Koval AC; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Young AL; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Fish JH; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Wallace J; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Chaney E; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Ushay G; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Ross RS; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Vostal EM; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Thisner MC; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Gonet KE; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Deane OC; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Pelletiere KR; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Rockafeller VC; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Waterman M; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Barry TW; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Goering CC; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Shipman SD; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Shiers AC; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Reilly CE; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Duff AM; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Madruga SL; Wildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA., Shirley DJ; Department of Engineering, Faraday, Inc., Burlington, VT, 05405, USA., Jerome KR; Virology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, 98109, USA., Pérez-Osorio AC; Virology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, USA., Greninger AL; Virology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, 98109, USA., Fortin N; Fish and Wildlife Department, Vermont Agency of Natural Resources, Rutland, VT, 05701, USA., Mosher BA; Rubenstein School of Environment and Natural Resources, University of Vermont, 81 Carrigan Dr, Burlington, VT, 05405, USA. Brittany.mosher@uvm.edu., Bruce EA; Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, VT, 05405, USA. Emily.bruce@med.uvm.edu. |
| المصدر: | Scientific reports [Sci Rep] 2023 Sep 06; Vol. 13 (1), pp. 14683. Date of Electronic Publication: 2023 Sep 06. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
| مواضيع طبية MeSH: | COVID-19*/epidemiology , Deer* , Coyotes* , Lynx* , Otters*, Animals ; Animals, Wild ; RNA, Viral/genetics ; SARS-CoV-2/genetics ; Vermont/epidemiology ; Foxes |
| مستخلص: | Previous studies have documented natural infections of SARS-CoV-2 in various domestic and wild animals. More recently, studies have been published noting the susceptibility of members of the Cervidae family, and infections in both wild and captive cervid populations. In this study, we investigated the presence of SARS-CoV-2 in mammalian wildlife within the state of Vermont. 739 nasal or throat samples were collected from wildlife throughout the state during the 2021 and 2022 harvest season. Data was collected from red and gray foxes (Vulpes vulples and Urocyon cineroargentus, respectively), fishers (Martes pennati), river otters (Lutra canadensis), coyotes (Canis lantrans), bobcats (Lynx rufus rufus), black bears (Ursus americanus), and white-tailed deer (Odocoileus virginianus). Samples were tested for the presence of SARS-CoV-2 via quantitative RT-qPCR using the CDC N1/N2 primer set and/or the WHO-E gene primer set. Surprisingly, we initially detected a number of N1 and/or N2 positive samples with high cycle threshold values, though after conducting environmental swabbing of the laboratory and verifying with a second independent primer set (WHO-E) and PCR without reverse transcriptase, we showed that these were false positives due to plasmid contamination from a construct expressing the N gene in the general laboratory environment. Our final results indicate that no sampled wildlife were positive for SARS-CoV-2 RNA, and highlight the importance of physically separate locations for the processing of samples for surveillance and experiments that require the use of plasmid DNA containing the target RNA sequence. These negative findings are surprising, given that most published North America studies have found SARS-CoV-2 within their deer populations. The absence of SARS-CoV-2 RNA in populations sampled here may provide insights in to the various environmental and anthropogenic factors that reduce spillover and spread in North American's wildlife populations. (© 2023. Springer Nature Limited.) |
| التعليقات: | Update of: bioRxiv. 2023 Apr 26;:. (PMID: 37162835) |
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| معلومات مُعتمدة: | P30 GM118228 United States GM NIGMS NIH HHS |
| المشرفين على المادة: | 0 (RNA, Viral) |
| SCR Organism: | Odocoileus virginianus |
| تواريخ الأحداث: | Date Created: 20230906 Date Completed: 20230908 Latest Revision: 20240603 |
| رمز التحديث: | 20240603 |
| مُعرف محوري في PubMed: | PMC10482933 |
| DOI: | 10.1038/s41598-023-39232-0 |
| PMID: | 37674004 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 2045-2322 |
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| DOI: | 10.1038/s41598-023-39232-0 |