دورية أكاديمية
Short communication: ROS production and mitochondrial membrane potential in hemocytes of marine bivalves, Mytilus galloprovincialis and Magallana gigas, under hypoosmotic stress.
| العنوان: | Short communication: ROS production and mitochondrial membrane potential in hemocytes of marine bivalves, Mytilus galloprovincialis and Magallana gigas, under hypoosmotic stress. |
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| المؤلفون: | Kladchenko ES; Laboratory of Ecological Immunology of Aquatic Organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Leninsky ave 14, Moscow, Russia, 119991. Electronic address: Kladchenko_ekaterina@bk.ru., Tkachuk AA; Laboratory of Ecological Immunology of Aquatic Organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Leninsky ave 14, Moscow, Russia, 119991., Podolskaya MS; Laboratory of Ecological Immunology of Aquatic Organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Leninsky ave 14, Moscow, Russia, 119991., Andreyeva AY; Laboratory of Ecological Immunology of Aquatic Organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Leninsky ave 14, Moscow, Russia, 119991. |
| المصدر: | Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [Comp Biochem Physiol B Biochem Mol Biol] 2024 Jan; Vol. 269, pp. 110901. Date of Electronic Publication: 2023 Sep 07. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Country of Publication: England NLM ID: 9516061 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1107 (Electronic) Linking ISSN: 10964959 NLM ISO Abbreviation: Comp Biochem Physiol B Biochem Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Pergamon Original Publication: Oxford : Tarrytown, NY : Pergamon ; Elsevier, c1994- |
| مواضيع طبية MeSH: | Mytilus*/metabolism , Ostreidae*, Animals ; Reactive Oxygen Species/metabolism ; Hemocytes/metabolism ; Membrane Potential, Mitochondrial ; Antioxidants/metabolism |
| مستخلص: | Bivalve mollusks that inhabit low-depth coastal and estuarine areas frequently experience osmotic stress that may be also associated with alterations of antioxidant enzyme activities and markers of oxidative stress. Mitochondria are a major source of reactive oxygen species (ROS) in eucaryotic cells. Overpoduction of ROS induces oxidative stress leading to a damage of intracellular compartments and cell death. In euryhaline bivalves, information concerning cellular ROS production upon osmotic stress and changes in mitochondrial membrane potential is scarce. The present study investigates osmotic stability and hemocytes` regulatory volume decrease (RVD) of Mediterranean mussel (Mytilus galloprovincialis) and the Pacific oyster (Magallana gigas). We also studied dynamic changes in intracellular ROS levels and mitochondrial membrane potential in hemocytes undergoing the RVD response following hypoosmotic swelling. Our data revealed that osmotic stability of mussel and oyster hemocytes did not significantly differ. Loss of environmental osmolarity from 460.0 ± 2.0 mOsm l -1 to 216.0 ± 4.0 mOsm l -1 resulted in an increase of hemocyte volume by 60% of the initial cellular volume in mussels and by 28% in oysters. After rapid hypoosmotic swelling hemocytes of both species demonstrated the RVD response. At the end of 60 min exposure to hypoosmotic environment, hemocyte volume significantly decreased in both species by 10-12% compared to the maximal hemocyte volume. Hypoosmotic shock induced an increase of mitochondrial membrane potential in hemocytes of mussels and oysters. In mussels, increased mitochondrial membrane potential was accompanied with decreased ROS levels in hemocytes, whereas oyster hemocytes showed enhanced ROS production. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Bivalves; Hemocyte; Mitochondrial membrane potential; Osmotic stability; Oxidative stress; Reactive oxygen species; Regulatory volume decrease |
| المشرفين على المادة: | 0 (Reactive Oxygen Species) 0 (Antioxidants) |
| تواريخ الأحداث: | Date Created: 20230908 Date Completed: 20231113 Latest Revision: 20231113 |
| رمز التحديث: | 20231113 |
| DOI: | 10.1016/j.cbpb.2023.110901 |
| PMID: | 37683884 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-1107 |
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| DOI: | 10.1016/j.cbpb.2023.110901 |