دورية أكاديمية
أعرض في EDS

Walnut Kernel Oil and Defatted Extracts Enhance Mesenchymal Stem Cell Stemness and Delay Senescence.

التفاصيل البيبلوغرافية
العنوان: Walnut Kernel Oil and Defatted Extracts Enhance Mesenchymal Stem Cell Stemness and Delay Senescence.
المؤلفون: Elsied MA; Anatomy and Embryology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt., Sharawi ZW; Biological Sciences Department, Faculty of Sciences, King AbdulAziz University, Jeddah 21589, Saudi Arabia., Al-Amrah H; Biological Sciences Department, Faculty of Sciences, King AbdulAziz University, Jeddah 21589, Saudi Arabia., Hegazy RA; Department of Biology, Al-Darb University College, Jazan University, Jazan 45142, Saudi Arabia., Mohamed AE; Biochemistry Division, Chemistry Department, Faculty of Science, Tanta University, Tanta 31527, Egypt., Saleh RM; Department of Physiology, Faculty of Veterinary Medicine, Mansura University, Mansura 35516, Egypt., El-Kholy SS; Department of Physiology, Faculty of Medicine, Kafrelsheikh University, Kafr El-Shaikh 33516, Egypt., Farrag FA; Anatomy and Embryology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt., Fayed MH; Anatomy and Embryology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt., El-Magd MA; Anatomy and Embryology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.
المصدر: Molecules (Basel, Switzerland) [Molecules] 2023 Aug 28; Vol. 28 (17). Date of Electronic Publication: 2023 Aug 28.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, c1995-
مواضيع طبية MeSH: AMP-Activated Protein Kinases* , Juglans*, Animals ; Rats ; Antioxidants/pharmacology ; Hydrogen Peroxide ; Sirtuin 1/genetics ; Tumor Suppressor Protein p53
مستخلص: Decreased stemness and increased cellular senescence impair the ability of mesenchymal stem cells (MSCs) to renew themselves, change into different cell types, and contribute to regenerative medicine. There is an urgent need to discover new compounds that can boost MSCs' stemness and delay senescence. Therefore, this study aimed to investigate the impact of walnut kernel oil (WKO) and defatted (WKD) extracts on bone marrow (BM)-MSC stemness and senescence. Premature senescence and inflammation were induced in BM-MSCs using H 2 O 2 and LPS, respectively. Phytochemical constituents of WKO and WKD extracts were detected by HPLC. The stemness (proliferation and migration), senescence-related markers (p53, p21, SIRT1, and AMPK), oxidative stress/antioxidant markers, inflammatory cytokines, and cell cycle of BM-MSCs were measured by MTT assay, qPCR, ELISA, and flow cytometry. WKO and WKD extracts improved rat BM-MSC stemness, as evidenced by (1) increased cell viability, (2) decreased apoptosis (low levels of Bax and caspase3 and high levels of Bcl2 ), (3) upregulated MMP9 and downregulated TIMP1 expression, and (4) cell cycle arrest in the G0/G1 phase and declined cell number in the S and G2/M phases. Additionally, WKO and WKD extracts reduced rat BM-MSC senescence, as indicated by (1) decreased p53 and p21 expression, (2) upregulated expression and levels of SIRT1 and AMPK, (3) reduced levels of ROS and improved antioxidant activity (higher activity of CAT, SOD, and GPx and upregulated expression of NrF2 and HO-1 ), and (4) declined levels of TNFα, IL1β, and NF-κB. When compared to the WKO extract, the WKD extract had a greater impact on the induction of stemness and reduction of senescence of BM-MSCs due to its stronger antioxidant activity, which could be attributed to its higher levels of flavonoids and phenolic compounds, as detected by HPLC analysis. WKO and WKD extracts enhance rat BM-MSC stemness and protect them from senescence, suggesting their potential use as enhancers to increase MSCs' therapeutic efficacy.
References: Cell Prolif. 2011 Jun;44(3):234-43. (PMID: 21535264)
Biomed Pharmacother. 2023 Jun;162:114356. (PMID: 37040673)
EMBO Mol Med. 2013 Mar;5(3):430-40. (PMID: 23364955)
J Bone Miner Res. 2013 Apr;28(4):960-9. (PMID: 23172686)
Nat Protoc. 2009;4(1):102-6. (PMID: 19131962)
Food Chem Toxicol. 2012 Mar;50(3-4):590-6. (PMID: 22266044)
Nat Rev Mol Cell Biol. 2008 May;9(5):367-77. (PMID: 18401346)
Biomed Res Int. 2014;2014:946859. (PMID: 24895630)
Immunology. 2015 Aug;145(4):455-67. (PMID: 25890999)
Ren Fail. 2022 Dec;44(1):171-183. (PMID: 35166167)
Biomedicine (Taipei). 2019 Dec;9(4):24. (PMID: 31724939)
Food Sci Biotechnol. 2016 Apr 30;25(2):623-629. (PMID: 30263315)
Nutrients. 2016 Jul 21;8(7):. (PMID: 27455311)
J Biol Chem. 2014 Aug 8;289(32):22048-62. (PMID: 24962570)
Ann Intern Med. 2006 Apr 18;144(8):554-62. (PMID: 16618952)
J Sci Food Agric. 2010 Sep;90(12):1959-67. (PMID: 20586084)
Toxicol Sci. 2013 Jan;131(1):56-70. (PMID: 22956630)
Molecules. 2020 May 07;25(9):. (PMID: 32392837)
Am J Clin Nutr. 2008 Mar;87(3):778-84. (PMID: 18326618)
Biol Pharm Bull. 2011;34(6):925-8. (PMID: 21628897)
Molecules. 2022 Nov 14;27(22):. (PMID: 36431959)
Mol Cell Biol. 2011 Nov;31(22):4593-608. (PMID: 21930787)
Inflammation. 2020 Feb;43(1):241-250. (PMID: 31741196)
Food Chem Toxicol. 2010 Jan;48(1):441-7. (PMID: 19883717)
Int J Mol Sci. 2021 Mar 05;22(5):. (PMID: 33807864)
Molecules. 2021 May 31;26(11):. (PMID: 34072744)
Molecules. 2015 Aug 26;20(9):15572-88. (PMID: 26343618)
J Endod. 2018 Apr;44(4):576-584. (PMID: 29306537)
Int J Urol. 2020 Nov;27(11):1039-1049. (PMID: 32794300)
Biomed Pharmacother. 2019 Jun;114:108732. (PMID: 30925457)
Antioxidants (Basel). 2020 May 14;9(5):. (PMID: 32423149)
Cytotherapy. 2015 Apr;17(4):443-53. (PMID: 25536864)
Int J Radiat Biol. 2005 Jun;81(6):445-58. (PMID: 16308915)
Bone. 2020 Apr;133:115252. (PMID: 31978617)
J Nutr. 1974 May;104(5):580-7. (PMID: 4823943)
J Natl Cancer Inst. 2010 Oct 20;102(20):1536-46. (PMID: 20858887)
J Cell Physiol. 2021 Jun;236(6):4290-4302. (PMID: 33421145)
Philos Trans R Soc Lond B Biol Sci. 2015 Oct 19;370(1680):20140376. (PMID: 26416685)
Stem Cells Int. 2018 Aug 27;2018:8058979. (PMID: 30224923)
Nat Commun. 2010 Apr 12;1:3. (PMID: 20975665)
J Biomed Mater Res A. 2022 Jan;110(1):21-30. (PMID: 34228402)
J Acupunct Meridian Stud. 2017 Jun;10(3):193-203. (PMID: 28712479)
Biochem Biophys Res Commun. 2018 Jun 7;500(3):682-690. (PMID: 29678576)
J Cardiovasc Nurs. 2009 Mar-Apr;24(2):98-103; quiz 104-5. (PMID: 19242274)
فهرسة مساهمة: Keywords: antioxidant; mesenchymal stem cells; senescence; stemness; walnut kernel extracts
المشرفين على المادة: EC 2.7.11.31 (AMP-Activated Protein Kinases)
0 (Antioxidants)
BBX060AN9V (Hydrogen Peroxide)
EC 3.5.1.- (Sirtuin 1)
0 (Tumor Suppressor Protein p53)
تواريخ الأحداث: Date Created: 20230909 Date Completed: 20230911 Latest Revision: 20230911
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10488345
DOI: 10.3390/molecules28176281
PMID: 37687109
قاعدة البيانات: MEDLINE
الوصف
تدمد:1420-3049
DOI:10.3390/molecules28176281