دورية أكاديمية
أعرض في EDS

Differential innate immune responses of human macrophages and bronchial epithelial cells against Talaromyces marneffei .

التفاصيل البيبلوغرافية
العنوان: Differential innate immune responses of human macrophages and bronchial epithelial cells against Talaromyces marneffei .
المؤلفون: Tan Y-P; Department of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Pok Fu Lam, Hong Kong, China., Tsang C-C; Department of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Pok Fu Lam, Hong Kong, China.; School of Medical and Health Sciences, Tung Wah College , Homantin, Hong Kong, China., Chan K-F; Department of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Pok Fu Lam, Hong Kong, China., Fung S-L; Tuberculosis and Chest Medicine Unit, Grantham Hospital , Aberdeen, Hong Kong, China., Kok K-H; Department of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Pok Fu Lam, Hong Kong, China., Lau SKP; Department of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Pok Fu Lam, Hong Kong, China., Woo PCY; Department of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Pok Fu Lam, Hong Kong, China.; Doctoral Program in Translational Medicine and Department of Life Sciences, National Chung Hsing University , Taichung, Taiwan.; The iEGG and Animal Biotechnology Research Center, National Chung Hsing University , Taichung, Taiwan.
المصدر: MSphere [mSphere] 2023 Oct 24; Vol. 8 (5), pp. e0025822. Date of Electronic Publication: 2023 Sep 11.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101674533 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2379-5042 (Electronic) Linking ISSN: 23795042 NLM ISO Abbreviation: mSphere Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, DC : American Society for Microbiology, [2015]-
مواضيع طبية MeSH: Travel* , Travel-Related Illness*, Humans ; Macrophages/microbiology ; Immunity, Innate ; Cytokines/metabolism ; Epithelial Cells/metabolism
مستخلص: Talaromyces marneffei is a thermally dimorphic fungal pathogen endemic in Southeast Asia. As inhalation of airborne conidia is believed as the major infection route, airway epithelial cells followed by pulmonary macrophages are the first cell types which the fungus encounters inside the host. In this study, we established an in vitro infection model based on human peripheral blood-derived macrophages (hPBDMs) cultured with the supplementation of autologous plasma. Using this model, we determined the transcriptomic changes of hPBDMs in response to T. marneffei infection by quantitative real-time reverse-transcription polymerase chain reaction as well as high-throughput RNA sequencing. Results showed that T. marneffei infection could activate hPBDMs to the M1-like phenotype and trigger a potent induction of chemokine and pro-inflammatory cytokine production as well as the expression of other immunoregulatory genes. In contrast to hPBDMs, there was no detectable innate cytokine response against T. marneffei in human bronchial epithelial cells (hBECs). Using a green fluorescent protein-tagged T. marneffei strain and confocal microscopy, internalization of the fungus by hBECs was confirmed. Live cell imaging further demonstrated that the infected cells exhibited normal cellular physiology, especially that the process of cell division could be observed. Moreover, T. marneffei also survived better inside hBECs than hPBDMs. Our results illustrated a potential role of hBECs to serve as reservoir cells for T. marneffei to evade immunosurveillance by phagocytes, from which the fungus reactivates when the host immunity is weakened and causes infection. Such immunoevasion and reactivation may also help explain the long incubation period observed for talaromycosis, in particular the travel-related cases. IMPORTANCE Talaromyces marneffei is an important fungal pathogen especially in Southeast Asia. To understand the innate immune response to talaromycosis, a suitable infection model is needed. Here, we established an in vitro T. marneffei infection model using human peripheral blood-derived macrophages (hPBDMs). We then examined the transcriptomic changes of hPBDMs in response to T. marneffei infection with this model. We found that contact with T. marneffei could activate hPBDMs to the M1-like phenotype and induced mRNA expressions of five cytokines and eight immunoregulatory genes. Contrary to hPBDMs, such immunoresponse was not elicited in human bronchial epithelial cells (hBECs), despite normal physiology observed in infected cells. We also found that infected hBECs did not eliminate T. marneffei as efficiently as hPBDMs. Our observation suggested that hBECs may potentially serve as reservoir cells for T. marneffei to evade immunosurveillance. When the host immunity deteriorates later, then the fungus reactivates and causes infection.
Competing Interests: Patrick C. Y. Woo has provided scientific advisory/laboratory services for Gilead Sciences, Incorporated; International Health Management Associates, Incorporated; Merck & Corporation, Incorporated; Micología Molecular S.L.; and Pfizer, Incorporated. The other authors report no conflict of interest. The funding sources had no role in study design, data collection, analysis, interpretation, or writing of the report. The authors alone are responsible for the content and the writing of the manuscript.
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فهرسة مساهمة: Keywords: RNA sequencing; Talaromyces marneffei; bronchial epithelial cells; human peripheral blood-derived macrophages
المشرفين على المادة: 0 (Cytokines)
SCR Disease Name: talaromycosis
SCR Organism: Talaromyces marneffei
تواريخ الأحداث: Date Created: 20230911 Date Completed: 20231027 Latest Revision: 20231027
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10597461
DOI: 10.1128/msphere.00258-22
PMID: 37695039
قاعدة البيانات: MEDLINE
الوصف
تدمد:2379-5042
DOI:10.1128/msphere.00258-22