دورية أكاديمية
Cross species/genera transferability of simple sequence repeat markers, genetic diversity and population structure analysis in gladiolus ( Gladiolus × grandiflorus L.) genotypes.
| العنوان: | Cross species/genera transferability of simple sequence repeat markers, genetic diversity and population structure analysis in gladiolus ( Gladiolus × grandiflorus L.) genotypes. |
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| المؤلفون: | Hiremath V; Division of Floriculture and Landscaping, Indian Agricultural Research Institute, New Delhi, India., Singh KP; Division of Floriculture and Landscaping, Indian Agricultural Research Institute, New Delhi, India., Jain N; Division of Genetics, Indian Agricultural Research Institute, New Delhi, India., Swaroop K; Division of Floriculture and Landscaping, Indian Agricultural Research Institute, New Delhi, India., Jain PK; National Institute of Plant Biotechnology, New Delhi, India., Panwar S; Division of Floriculture and Landscaping, Indian Agricultural Research Institute, New Delhi, India., Sinha N; Division of Genetics, Indian Agricultural Research Institute, New Delhi, India. |
| المصدر: | PeerJ [PeerJ] 2023 Sep 07; Vol. 11, pp. e15820. Date of Electronic Publication: 2023 Sep 07 (Print Publication: 2023). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: PeerJ Inc Country of Publication: United States NLM ID: 101603425 Publication Model: eCollection Cited Medium: Internet ISSN: 2167-8359 (Electronic) Linking ISSN: 21678359 NLM ISO Abbreviation: PeerJ Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Corte Madera, CA : PeerJ Inc. |
| مواضيع طبية MeSH: | Iridaceae*/genetics , Crocus* , Iris Plant*, Humans ; Genotype ; Genetic Variation/genetics |
| مستخلص: | Background: Genetic analysis of gladiolus germplasm using simple sequence repeat (SSR) markers is largely missing due to scarce genomic information. Hence, microsatellites identified for related genera or species may be utilized to understand the genetic diversity and assess genetic relationships among cultivated gladiolus varieties. Methods: In the present investigation, we screened 26 genomic SSRs ( Gladiolus palustris, Crocus sativus , Herbertia zebrina , Sysirinchium micranthum ), 14 chloroplast SSRs (Gladiolus spp . , chloroplast DNA regions ) and 25 Iris Expressed Sequence Tags (ESTs) derived SSRs across the 84 gladiolus ( Gladiolus × grandiflorus L.) genotypes. Polymorphic markers detected from amplified SSRs were used to calculate genetic diversity estimates, analyze population structure, cluster analysis and principal coordinate analysis (PCoA). Results: A total of 41 SSRs showed reproducible amplification pattern among the selected gladiolus cultivars. Among these, 17 highly polymorphic SSRs revealed a total of 58 polymorphic alleles ranging from two to six with an average of 3.41 alleles per marker. Polymorphic information content (PIC) values ranged from 0.11 to 0.71 with an average value of 0.48. A total of 4 SSRs were selectively neutral based on the Ewens-Watterson test. Hence, 66.66% of Gladiolus palustris , 48% of Iris spp. EST, 71.42% of Crocus sativus SSRs showed cross-transferability among the gladiolus genotypes. Analysis of genetic structure of 84 gladiolus genotypes revealed two subpopulations; 35 genotypes were assigned to subpopulation 1, 37 to subpopulation 2 and the remaining 12 genotypes could not be attributed to either subpopulation. Analysis of molecular variance indicated maximum variance (53.59%) among individuals within subpopulations, whereas 36.55% of variation among individuals within the total population. The least variation (9.86%) was noticed between two subpopulations. Moderate (F Conclusion: SSR markers from the present study can be utilized for cultivar identification, conservation and sustainable utilization of gladiolus genotypes for crop improvement. Genetic relationships assessed among the genotypes of respective clusters may assist the breeders in selecting desirable parents for crossing. Competing Interests: The authors declare that they have no competing interests. (© 2023 Hiremath et al.) |
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| فهرسة مساهمة: | Keywords: Conservation; Cross genera; Cross species; Cross transferability; Genetic diversity; Genetic structure; Gladiolus; Heterozygosity; Microsatellites; Polymorphism |
| تواريخ الأحداث: | Date Created: 20230913 Date Completed: 20230914 Latest Revision: 20230915 |
| رمز التحديث: | 20230915 |
| مُعرف محوري في PubMed: | PMC10493085 |
| DOI: | 10.7717/peerj.15820 |
| PMID: | 37701831 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2167-8359 |
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| DOI: | 10.7717/peerj.15820 |