دورية أكاديمية
Evaluation of Xpert MTB/RIF Ultra for the Diagnosis of Extrapulmonary Tuberculosis: A Retrospective Analysis in Saudi Arabia.
العنوان: | Evaluation of Xpert MTB/RIF Ultra for the Diagnosis of Extrapulmonary Tuberculosis: A Retrospective Analysis in Saudi Arabia. |
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المؤلفون: | Slail MJ; Tuberculosis Department, Dammam Regional Laboratory, Ministry of Health, Dammam, Saudi Arabia., Booq RY; Wellness and Preventive Medicine Institute, Health Sector, King Abdulaziz City for Science and Technology (KACST), 11442, Riyadh, Saudi Arabia., Al-Ahmad IH; Tuberculosis Department, Dammam Regional Laboratory, Ministry of Health, Dammam, Saudi Arabia., Alharbi AA; Mycobacteriology Unit, Riyadh Regional Laboratory, Ministry of Health, 12746, Riyadh, Saudi Arabia., Alharbi SF; Mycobacteriology Unit, Riyadh Regional Laboratory, Ministry of Health, 12746, Riyadh, Saudi Arabia., Alotaibi MZ; Mycobacteriology Unit, Riyadh Regional Laboratory, Ministry of Health, 12746, Riyadh, Saudi Arabia., Aljubran AM; Mycobacteriology Unit, Riyadh Regional Laboratory, Ministry of Health, 12746, Riyadh, Saudi Arabia., Aldossary AM; Wellness and Preventive Medicine Institute, Health Sector, King Abdulaziz City for Science and Technology (KACST), 11442, Riyadh, Saudi Arabia., Memish ZA; College of Medicine, Alfaisal University, 11533, Riyadh, Saudi Arabia.; Research & Innovation Center, King Saud Medical City, Riyadh, Saudi Arabia.; Hubert Department School of Public Health, Emory University, Atlanta, USA.; Division of Infectious Diseases, Kyung Hee University, Seoul, Korea., Alyamani EJ; Wellness and Preventive Medicine Institute, Health Sector, King Abdulaziz City for Science and Technology (KACST), 11442, Riyadh, Saudi Arabia., Tawfik EA; Advanced Diagnostics and Therapeutics Institute, Health Sector, King Abdulaziz City for Science and Technology (KACST), 11442, Riyadh, Saudi Arabia. etawfik@kacst.edu.sa., Binjomah AZ; Mycobacteriology Unit, Riyadh Regional Laboratory, Ministry of Health, 12746, Riyadh, Saudi Arabia. abinjomah@moh.gov.sa.; College of Medicine, Alfaisal University, 11533, Riyadh, Saudi Arabia. abinjomah@moh.gov.sa. |
المصدر: | Journal of epidemiology and global health [J Epidemiol Glob Health] 2023 Dec; Vol. 13 (4), pp. 782-793. Date of Electronic Publication: 2023 Sep 14. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Springer Country of Publication: Switzerland NLM ID: 101592084 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2210-6014 (Electronic) Linking ISSN: 22106006 NLM ISO Abbreviation: J Epidemiol Glob Health Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2021- : [Cham] : Springer Original Publication: Amsterdam : Elsevier, [2011]- |
مواضيع طبية MeSH: | Tuberculosis, Pulmonary*/diagnosis , Mycobacterium tuberculosis*/genetics , Antibiotics, Antitubercular*/pharmacology , Tuberculosis, Extrapulmonary*, Humans ; Rifampin/therapeutic use ; Retrospective Studies ; Saudi Arabia ; Sensitivity and Specificity ; Drug Resistance, Bacterial ; Sputum/microbiology |
مستخلص: | The incidence of extrapulmonary tuberculosis (EPTB) in low- and middle-income countries, as well as, high-income countries has increased over the last two decades. The acid-fast bacillus (AFB) smear test is easy to perform and cost-effective with a quick turnaround time but the test has low sensitivity. Culture remains the gold standard for detecting TB; however, it has low sensitivity and slow bacterial growth patterns, as it may take up to 6 to 8 weeks to grow. Therefore, a rapid detection tool is crucial for the early initiation of treatment and ensuring an improved therapeutic outcome. Here, the Xpert Ultra system was developed as a nucleic acid amplification technique to accelerate the detection of MTB in paucibacillary clinical samples and endorsed by the World Health Organization. From March 2020 to August 2021, Xpert Ultra was evaluated for its sensitivity and specificity against EPTB and compared with those of the routinely used Xpert, culture, and AFB tests in 845 clinical samples in Saudi Arabia. The results indicate the overall sensitivity and specificity of Xpert Ultra to be 91% and 95%, respectively, compared with the Xpert (82% and 99%, respectively) and AFB smear (18% and 100%, respectively) tests. The results also indicated that despite the low microbial loads that were categorized as trace, very low, or low on Xpert Ultra, yet, complete detection was achieved with some sample types (i.e., 100% detection). Consequently, Xpert Ultra has great potential to replace conventional diagnostic approaches as a standard detection method for EPTB. (© 2023. The Author(s).) |
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فهرسة مساهمة: | Keywords: Extrapulmonary tuberculosis; Pulmonary tuberculosis; Rapid detection; Real-time polymerase chain reaction (RT-PCR); Saudi Arabia; Xpert Ultra |
المشرفين على المادة: | VJT6J7R4TR (Rifampin) 0 (Antibiotics, Antitubercular) |
تواريخ الأحداث: | Date Created: 20230914 Date Completed: 20231130 Latest Revision: 20231201 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC10686912 |
DOI: | 10.1007/s44197-023-00150-z |
PMID: | 37707714 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2210-6014 |
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DOI: | 10.1007/s44197-023-00150-z |