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Effect of silymarin on the relative gene expressions of some inflammatory cytokines in the liver of CCl 4 -intoxicated male rats.

التفاصيل البيبلوغرافية
العنوان: Effect of silymarin on the relative gene expressions of some inflammatory cytokines in the liver of CCl 4 -intoxicated male rats.
المؤلفون: El-Kot SM; Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, 21568, Egypt. sarah.maher_PG@alexu.edu.eg.; Environmental Studies Department, Institute of Graduate Studies and Research, Alexandria University, Alexandria, 21526, Egypt. sarah.maher_PG@alexu.edu.eg., Wanas W; Materials Science Department, Institute of Graduate Studies and Research, Alexandria University, Alexandria, 21526, Egypt., Hafez AM; Environmental Studies Department, Institute of Graduate Studies and Research, Alexandria University, Alexandria, 21526, Egypt., Mahmoud NA; Physiology Department, Faculty of Medicine (Girls), Al-Azhar University, Cairo, 11754, Egypt., Tolba AM; Anatomy Department, Faculty of Medicine (Girls), Al-Azhar University, Cairo, 11754, Egypt., Younis AH; National Institute of Oceanography and Fisheries (NIOF), Alexandria, 21556, Egypt., Sayed GE; Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, 21568, Egypt.; Waste Water Lab, Baheria Water and Waste Company, Baheria, Damanhur, 107, Egypt., Abdelwahab HE; Environmental Studies Department, Institute of Graduate Studies and Research, Alexandria University, Alexandria, 21526, Egypt.; Chemistry Department, Faculty of Science, Alexandria University, Alexandria, 21568, Egypt.
المصدر: Scientific reports [Sci Rep] 2023 Sep 14; Vol. 13 (1), pp. 15245. Date of Electronic Publication: 2023 Sep 14.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Cytokines* , Corn Oil*, Male ; Animals ; Rats ; Rats, Wistar ; Xenobiotics ; Liver ; Interleukin-6 ; Cyclooxygenase 2/genetics ; NF-kappa B ; Inflammation/chemically induced ; Inflammation/genetics
مستخلص: The intensive exposure of the liver cells to any type of noxae, such as viruses, drugs, alcohols, and xenobiotics could induce hepatic inflammation through the upregulation of gene expression of several fibrotic and inflammatory mediators. So, our study assessed the role of silymarin on the inflammatory response induced by carbon tetrachloride (CCl 4 ) as an example of xenobiotics on liver tissues in male rats. Forty-eight Wister male rats (weight: 130 ± 10) were housed for 14 days and then divided randomly into six groups: control, SLY: rats received only silymarin orally for 12 weeks (daily), CO: rats were injected with corn oil for 8 weeks (3 times weekly), CCl 4 : rats were injected with CCl 4 solubilized in corn oil for 8 weeks (day by day), Treated: rats received silymarin for 4 weeks after CCl 4 injection, Protected: rats received silymarin for 4 weeks before and 8 weeks during CCl 4 injection. When the treatment period for the rats was over, they underwent scarification after anesthesia. Then, the sera were extracted from the collected blood for the determination of irisin levels, liver functions, and lipid profiles. Liver tissues were separated for the histopathological examinations, the determination of oxidative stress (OS) parameters content, and the relative gene expression of inflammatory cytokines; nuclear factor kappa (NF)-κB, tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, cyclooxygenase (COX)-2, and transforming growth factor beta (TGF-β). The findings showed that silymarin reduced liver inflammation by overcoming the OS process and inflammatory cytokines production which was stimulated by CCl 4 . These results were confirmed by histopathology of liver tissues.
(© 2023. Springer Nature Limited.)
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المشرفين على المادة: 0 (Cytokines)
8001-30-7 (Corn Oil)
0 (Xenobiotics)
0 (Interleukin-6)
EC 1.14.99.1 (Cyclooxygenase 2)
0 (NF-kappa B)
تواريخ الأحداث: Date Created: 20230914 Date Completed: 20230918 Latest Revision: 20231120
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10502111
DOI: 10.1038/s41598-023-42250-7
PMID: 37710007
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-023-42250-7