دورية أكاديمية
Macrophage phenotype impacts in vitro equine intrasynovial deep digital flexor tenocyte matrix metalloproteinase gene expression and secretion.
| العنوان: | Macrophage phenotype impacts in vitro equine intrasynovial deep digital flexor tenocyte matrix metalloproteinase gene expression and secretion. |
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| المؤلفون: | Cooper HE, Bowlby C, Long S, Durgam SS |
| المصدر: | American journal of veterinary research [Am J Vet Res] 2023 Sep 19; Vol. 84 (12). Date of Electronic Publication: 2023 Sep 19 (Print Publication: 2023). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Veterinary Medical Association Country of Publication: United States NLM ID: 0375011 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1943-5681 (Electronic) Linking ISSN: 00029645 NLM ISO Abbreviation: Am J Vet Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Schaumburg, Ill. : American Veterinary Medical Association Original Publication: Chicago : American Veterinary Medical Assn. |
| مواضيع طبية MeSH: | Matrix Metalloproteinase 1* , Matrix Metalloproteinase 9*, Animals ; Horses ; Tenocytes/chemistry ; Tenocytes/metabolism ; Tissue Inhibitor of Metalloproteinase-1/genetics ; Macrophages ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Gene Expression ; Phenotype ; Culture Media/metabolism ; Cells, Cultured |
| مستخلص: | Objective: To investigate matrix metalloproteinase (MMP) and their inhibitors tissue inhibitor matrix metalloproteinase (TIMP) gene expression and secretion during equine deep digital flexor tendon (DDFT) tenocyte and macrophage (undifferentiated, proinflammatory, and regulatory) co-culture. Sample: Third passage DDF tenocytes and donor-matched macrophages differentiated from peripheral blood CD14+ monocytes from 5 healthy horses ages 9-11 years, euthanized for reasons unrelated to musculoskeletal conditions. Methods: Passage 3 DDT tenocyte aggregate cultures were co-cultured with undifferentiated (control), proinflammatory (granulocyte-macrophage colony-stimulating factor; GM-CSF pretreated and lipopolysaccharide + interferon gamma-primed; LPS+IFN-γ) or regulatory (interleukin-4 and interleukin-10-primed; IL-4 + IL-10) macrophages in direct and transwell co-cultures for 72 hours. MMP-1, -2, -3, -9, -13, and TIMP -1, -2 mRNA were measured via real-time Polymerase Chain Reaction (rtPCR). Co-culture media MMP -3, -9, and TIMP -1, -2 concentrations were quantified via ELISA. Results: Direct co-culture of DDF tenocytes with proinflammatory macrophages for 72 hours increased MMP-1, -3, and -13 mRNA levels whereas, MMP-9 mRNA levels decreased. Direct and transwell co-culture with proinflammatory and regulatory macrophages resulted in increased MMP-3 and decreased MMP-9 media concentrations. While direct co-culture with regulatory macrophages significantly increased TIMP-1 mRNA, overall, TIMP mRNA and culture media concentrations were largely unchanged. Clinical Relevance: Cell-to-cell contact between DDF tenocytes and macrophages is not essential to induce MMP gene expression and secretion. Co-culture systems offer a viable in vitro platform to screen and evaluate immunomodulatory properties of therapies aimed at improving equine intrasynovial tendon healing. |
| فهرسة مساهمة: | Keywords: MMP; TIMP; co-culture; deep digital flexor tenocytes; equine |
| المشرفين على المادة: | EC 3.4.24.7 (Matrix Metalloproteinase 1) EC 3.4.24.35 (Matrix Metalloproteinase 9) 0 (Tissue Inhibitor of Metalloproteinase-1) 0 (RNA, Messenger) 0 (Culture Media) |
| تواريخ الأحداث: | Date Created: 20230915 Date Completed: 20231204 Latest Revision: 20231204 |
| رمز التحديث: | 20231215 |
| DOI: | 10.2460/ajvr.23.05.0106 |
| PMID: | 37714521 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1943-5681 |
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| DOI: | 10.2460/ajvr.23.05.0106 |