دورية أكاديمية

Reactive Oxygen Species-Mediated Mitophagy and Cell Apoptosis are Involved in the Toxicity of Aluminum Chloride Exposure in GC-2spd.

التفاصيل البيبلوغرافية
العنوان: Reactive Oxygen Species-Mediated Mitophagy and Cell Apoptosis are Involved in the Toxicity of Aluminum Chloride Exposure in GC-2spd.
المؤلفون: Peng HX; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China., Chai F; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China., Chen KH; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China., Huang YX; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China., Wei GJ; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China., Yuan H; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China., Pang YF; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China., Luo SH; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. shihualuo99@126.com.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. shihualuo99@126.com., Wang CF; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. chunfang.wang@ymun.edu.cn.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. chunfang.wang@ymun.edu.cn., Chen WC; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. chwch3268@sina.com.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. chwch3268@sina.com.
المصدر: Biological trace element research [Biol Trace Elem Res] 2024 Jun; Vol. 202 (6), pp. 2616-2629. Date of Electronic Publication: 2023 Sep 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Humana Press Country of Publication: United States NLM ID: 7911509 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-0720 (Electronic) Linking ISSN: 01634984 NLM ISO Abbreviation: Biol Trace Elem Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London, Clifton, N. J.] Humana Press.
مواضيع طبية MeSH: Aluminum Chloride*/toxicity , Apoptosis*/drug effects , Reactive Oxygen Species*/metabolism , Mitophagy*/drug effects, Animals ; Male ; Mice ; Membrane Potential, Mitochondrial/drug effects ; Testis/drug effects ; Testis/metabolism ; Testis/pathology ; Cell Survival/drug effects ; Spermatocytes/drug effects ; Spermatocytes/metabolism ; Cell Line ; Chlorides/toxicity ; Aluminum Compounds/toxicity
مستخلص: Aluminum chloride is an inorganic polymeric coagulant commonly found in daily life and various materials. Although male reproductive toxicity has been associated with AlCl 3 exposure, the underlying mechanism remains unclear. This study aimed to examine the impact of AlCl 3 exposure on mitophagy and mitochondrial apoptosis in testicular tissue and mouse spermatocytes. Reactive oxygen species (ROS) and ATP levels were measured in GC-2spd after AlCl 3 exposure using a multifunctional enzyme labeler. The changes in mitochondrial membrane potential (MMP) and TUNEL were observed through confocal laser microscopy, and the expression of proteins associated with mitophagy and apoptosis was analyzed using Western blot. Our results demonstrated that AlCl 3 exposure disrupted mitophagy and increased apoptosis-related protein expression in testicular tissues. In the in vitro experiments, AlCl 3 exposure induced ROS production, suppressed cell viability and ATP production, and caused a decrease in MMP, leading to mitophagy and cell apoptosis in GC-2spd cells. Intervention with N-acetylcysteine (NAC) reduced ROS production and partially restored mitochondrial function, thereby reversing the resulting mitophagy and cell apoptosis. Our findings provide evidence that ROS-mediated mitophagy and cell apoptosis play a crucial role in the toxicity of AlCl 3 exposure in GC-2spd. These results contribute to the understanding of male reproductive toxicity caused by AlCl 3 exposure and offer a foundation for future research in this area.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
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معلومات مُعتمدة: 2020KY13017 Guangxi University Young and Middle-aged Teachers' Basic Ability Improvement Project; GZZC2020248 Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine Self-financing Scientific Research Project; Z20201416 Guangxi Zhuang Autonomous Region Health and Health Commission Self-financing Scientific Research Course; 81960303 National Natural Science Foundation of China; 2020GXNSFAA297257) Guangxi Natural Science Foundation Project
فهرسة مساهمة: Keywords: Aluminum chloride; Apoptosis; Mitophagy; Mouse spermatocyte; Reproductive toxicity
المشرفين على المادة: 3CYT62D3GA (Aluminum Chloride)
0 (Reactive Oxygen Species)
0 (Chlorides)
0 (Aluminum Compounds)
تواريخ الأحداث: Date Created: 20230915 Date Completed: 20240426 Latest Revision: 20240426
رمز التحديث: 20240427
DOI: 10.1007/s12011-023-03848-0
PMID: 37715092
قاعدة البيانات: MEDLINE
الوصف
تدمد:1559-0720
DOI:10.1007/s12011-023-03848-0