دورية أكاديمية
Does ghrelin regulate intestinal motility in rabbits? An in vitro study using isolated duodenal strips.
| العنوان: | Does ghrelin regulate intestinal motility in rabbits? An in vitro study using isolated duodenal strips. |
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| المؤلفون: | Zhang S; Laboratory of Veterinary Physiology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China; School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan., Kaiya H; Department of Biochemistry, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka 564-8565, Japan; Faculty of Science, University of Toyama, Toyama, Toyama 933-8555, Japan; Grandsoul Research Institute for Immunology, Inc., Uda, Nara 633-2221, Japan., Kitazawa T; School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan. Electronic address: ktz-tko@rakuno.ac.jp. |
| المصدر: | General and comparative endocrinology [Gen Comp Endocrinol] 2023 Dec 01; Vol. 344, pp. 114384. Date of Electronic Publication: 2023 Sep 16. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0370735 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-6840 (Electronic) Linking ISSN: 00166480 NLM ISO Abbreviation: Gen Comp Endocrinol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York, NY : Academic Press Original Publication: New York. |
| مواضيع طبية MeSH: | Ghrelin*/pharmacology , Motilin*/pharmacology, Rabbits ; Rats ; Animals ; Receptors, Ghrelin/genetics ; Duodenum ; Gastrointestinal Motility ; Muscle Contraction ; RNA, Messenger |
| مستخلص: | Rabbit duodenum has been used for examining the ability of motilin to cause muscle contraction in vitro. A motilin-related peptide, ghrelin, is known to be involved in the regulation of gastrointestinal (GI) motility in various animals, but its ability to cause rabbit GI contraction have not been well examined. The aim of this study is to clarify the action of rat ghrelin and its interaction with motilin in the rabbit duodenum. The mRNA expression of ghrelin and motilin receptors was also examined using RT-PCR. Rat ghrelin (10 -9 -10 -6 M) did not change the contractile activity of the duodenum measured by the mean muscle tonus and area under the curve of contraction waves. In agreement with this result, the distribution of ghrelin receptor mRNA in the rabbit GI tract varied depending on the GI region from which the samples were taken; the expression level in the duodenum was negligible, but that in the esophagus or stomach was significant. On the other hand, motilin (10 -10 -10 -6 M) caused a concentration-dependent contraction by means of increased mean muscle tonus, and consistently, motilin receptor mRNA was expressed heterogeneously depending on the GI region (esophagus = stomach = colon = rectum < duodenum = jejunum = ileum < cecum). Expression level of motilin receptor was comparable to that of ghrelin receptor in the esophagus and stomach. Pretreatment with ghrelin (10 -6 M) prior to motilin did not affect the contractile activity of motilin in the duodenum. In conclusion, ghrelin does not affect muscle contractility or motilin-induced contraction in the rabbit duodenum, which is due to the lack of ghrelin receptors. The present in vitro results suggest that ghrelin might not be a regulator of intestinal motility in rabbits. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Contractility; Ghrelin; Interaction; Motilin; Rabbit duodenum |
| المشرفين على المادة: | 0 (Ghrelin) 52906-92-0 (Motilin) 0 (Receptors, Ghrelin) 0 (RNA, Messenger) |
| تواريخ الأحداث: | Date Created: 20230918 Date Completed: 20231106 Latest Revision: 20231113 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1016/j.ygcen.2023.114384 |
| PMID: | 37722460 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1095-6840 |
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| DOI: | 10.1016/j.ygcen.2023.114384 |