Single-cell multi-omics sequencing of human spermatogenesis reveals a DNA demethylation event associated with male meiotic recombination.

التفاصيل البيبلوغرافية
العنوان:	Single-cell multi-omics sequencing of human spermatogenesis reveals a DNA demethylation event associated with male meiotic recombination.
المؤلفون:	Huang Y; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China., Li L; Guangdong Provincial Key Laboratory of Proteomics, Department of Pathophysiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China., An G; Department of Reproductive Medicine Center, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, P. R. China., Yang X; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China., Cui M; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China., Song X; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China., Lin J; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China., Zhang X; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China., Yao Z; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China., Wan C; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China., Zhou C; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China., Zhao J; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China., Song K; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China., Ren S; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China., Xia X; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China., Fu X; Department of Reproductive Medicine Center, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, P. R. China., Lan Y; Department of Reproductive Medicine Center, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, P. R. China., Hu X; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China., Wang W; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China., Wang M; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China., Zheng Y; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China., Miao K; Centre for Precision Medicine Research and Training, Faculty of Health Sciences, University of Macau, Macau, P. R. China., Bai X; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China., Hutchins AP; Department of Systems Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen, P. R. China., Chang G; Department of Biochemistry and Molecular Biology, Shenzhen University Medical School, Shenzhen, P. R. China. changgang@szu.edu.cn., Gao S; State Key Laboratory of Animal Biotech Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of the MARA, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, P. R. China. gaoshuai1959@163.com., Zhao XY; State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R. China. zhaoxiaoyang@smu.edu.cn.; Guangdong Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangzhou, P. R. China. zhaoxiaoyang@smu.edu.cn.; Key Laboratory of Mental Health of the Ministry of Education, Guangzhou, P. R. China. zhaoxiaoyang@smu.edu.cn.; Department of Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou, P. R. China. zhaoxiaoyang@smu.edu.cn.; National Clinical Research Center for Kidney Disease, Guangzhou, P. R. China. zhaoxiaoyang@smu.edu.cn.
المصدر:	Nature cell biology [Nat Cell Biol] 2023 Oct; Vol. 25 (10), pp. 1520-1534. Date of Electronic Publication: 2023 Sep 18.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Macmillan Magazines Ltd Country of Publication: England NLM ID: 100890575 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-4679 (Electronic) Linking ISSN: 14657392 NLM ISO Abbreviation: Nat Cell Biol Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: London : Macmillan Magazines Ltd., [1999-
مواضيع طبية MeSH:	DNA Demethylation* , Multiomics*, Humans ; Male ; Animals ; Mice ; Spermatogenesis/genetics ; Meiosis/genetics ; Chromatin/genetics
مستخلص:	Human spermatogenesis is a highly ordered process; however, the roles of DNA methylation and chromatin accessibility in this process remain largely unknown. Here by simultaneously investigating the chromatin accessibility, DNA methylome and transcriptome landscapes using the modified single-cell chromatin overall omic-scale landscape sequencing approach, we revealed that the transcriptional changes throughout human spermatogenesis were correlated with chromatin accessibility changes. In particular, we identified a set of transcription factors and cis elements with potential functions. A round of DNA demethylation was uncovered upon meiosis initiation in human spermatogenesis, which was associated with male meiotic recombination and conserved between human and mouse. Aberrant DNA hypermethylation could be detected in leptotene spermatocytes of certain nonobstructive azoospermia patients. Functionally, the intervention of DNA demethylation affected male meiotic recombination and fertility. Our work provides multi-omics landscapes of human spermatogenesis at single-cell resolution and offers insights into the association between DNA demethylation and male meiotic recombination. (© 2023. The Author(s), under exclusive licence to Springer Nature Limited.)
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المشرفين على المادة:	0 (Chromatin)
تواريخ الأحداث:	Date Created: 20230918 Date Completed: 20231221 Latest Revision: 20231221
رمز التحديث:	20231221
DOI:	10.1038/s41556-023-01232-7
PMID:	37723297
قاعدة البيانات:	MEDLINE

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تدمد:	1476-4679
DOI:	10.1038/s41556-023-01232-7