دورية أكاديمية
Custom tuning of Rieske oxygenase reactivity.
| العنوان: | Custom tuning of Rieske oxygenase reactivity. |
|---|---|
| المؤلفون: | Tian J; Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109, USA., Liu J; Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109, USA., Knapp M; Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109, USA., Donnan PH; Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109, USA., Boggs DG; Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109, USA., Bridwell-Rabb J; Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109, USA. jebridwe@umich.edu. |
| المصدر: | Nature communications [Nat Commun] 2023 Sep 20; Vol. 14 (1), pp. 5858. Date of Electronic Publication: 2023 Sep 20. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [London] : Nature Pub. Group |
| مواضيع طبية MeSH: | Oxygenases* , Dioxygenases*, Mixed Function Oxygenases ; Catalysis ; Culture |
| مستخلص: | Rieske oxygenases use a Rieske-type [2Fe-2S] cluster and a mononuclear iron center to initiate a range of chemical transformations. However, few details exist regarding how this catalytic scaffold can be predictively tuned to catalyze divergent reactions. Therefore, in this work, using a combination of structural analyses, as well as substrate and rational protein-based engineering campaigns, we elucidate the architectural trends that govern catalytic outcome in the Rieske monooxygenase TsaM. We identify structural features that permit a substrate to be functionalized by TsaM and pinpoint active-site residues that can be targeted to manipulate reactivity. Exploiting these findings allowed for custom tuning of TsaM reactivity: substrates are identified that support divergent TsaM-catalyzed reactions and variants are created that exclusively catalyze dioxygenation or sequential monooxygenation chemistry. Importantly, we further leverage these trends to tune the reactivity of additional monooxygenase and dioxygenase enzymes, and thereby provide strategies to custom tune Rieske oxygenase reaction outcomes. (© 2023. Springer Nature Limited.) |
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| معلومات مُعتمدة: | R35 GM138271 United States GM NIGMS NIH HHS |
| المشرفين على المادة: | EC 1.13.- (Oxygenases) EC 1.- (Mixed Function Oxygenases) EC 1.13.11.- (Dioxygenases) |
| تواريخ الأحداث: | Date Created: 20230920 Date Completed: 20230922 Latest Revision: 20240212 |
| رمز التحديث: | 20240212 |
| مُعرف محوري في PubMed: | PMC10511449 |
| DOI: | 10.1038/s41467-023-41428-x |
| PMID: | 37730711 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 2041-1723 |
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| DOI: | 10.1038/s41467-023-41428-x |