دورية أكاديمية
Validation of the use of dried blood spots in a chikungunya virus IgG serological assay.
| العنوان: | Validation of the use of dried blood spots in a chikungunya virus IgG serological assay. |
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| المؤلفون: | Magalhaes T; Department of Entomology, Texas A&M University, College Station, EUA, United States of America; Faculdade de Medicina da Bahia, Universidade Federal da Bahia, Salvador, Brazil., Portilho MM; Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BraZil., Moreira PSS; Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BraZil., Marinho ML; Instituto de Saúde Coletiva, Universidade Federal da Bahia, Salvador, Brazil., Dias WP; Instituto de Saúde Coletiva, Universidade Federal da Bahia, Salvador, Brazil., Gonçalves NM; Secretaria Municipal de Saúde de Salvador, Salvador, Brazil., Rodrigues OAS; Instituto de Saúde Coletiva, Universidade Federal da Bahia, Salvador, Brazil., Montes J; Instituto de Saúde Coletiva, Universidade Federal da Bahia, Salvador, Brazil., Reis L; Centro de Estudos Afro-Orientais, Universidade Federal da Bahia, Salvador, Brazil., Jesus DF; Secretaria Municipal de Saúde de Salvador, Salvador, Brazil., Silva TO; Instituto de Saúde Coletiva, Universidade Federal da Bahia, Salvador, Brazil., Dultra LS; Instituto de Saúde Coletiva, Universidade Federal da Bahia, Salvador, Brazil; Secretaria Municipal de Saúde de Salvador, Salvador, Brazil; Centro de Ciências da Saúde, Universidade Federal do Recôncavo da Bahia, Santo Antônio de Jesus, Brazil., Nery JS; Instituto de Saúde Coletiva, Universidade Federal da Bahia, Salvador, Brazil., Ribeiro GS; Faculdade de Medicina da Bahia, Universidade Federal da Bahia, Salvador, Brazil; Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BraZil. Electronic address: guilherme.ribeiro@fiocruz.br. |
| المصدر: | Journal of immunological methods [J Immunol Methods] 2023 Nov; Vol. 522, pp. 113571. Date of Electronic Publication: 2023 Sep 22. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 1305440 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7905 (Electronic) Linking ISSN: 00221759 NLM ISO Abbreviation: J Immunol Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Amsterdam, North-Holand, |
| مواضيع طبية MeSH: | Chikungunya virus*, Humans ; Sensitivity and Specificity ; Enzyme-Linked Immunosorbent Assay/methods ; Antibodies, Viral ; Immunoglobulin G ; Dried Blood Spot Testing/methods |
| مستخلص: | Dried blood spot (DBS) sampling is a simple, fast, and minimally invasive blood collection method that is particularly useful for diagnostic or epidemiological studies in hard-to-reach populations. Nevertheless, the use of DBS in assays that have been optimized with gold-standard samples (serum or plasma) must be optimized to yield reliable results. Here, we describe the validation of DBS in a commercial assay to measure IgG against chikungunya virus (CHIKV IgG ELISA; Euroimmun, Lübeck, Germany). During a health survey of people experiencing homelessness in Salvador, Brazil, between September 2021 and February 2022, a subset (75/523; 14.3%) of the study participants had paired capillary (for DBS preparation) and venous (for serum separation) blood samples collected. A pilot optimization test was initially performed with 17 paired samples to compare the CHIKV IgG ELISA absorbance values between serum and three different dilutions of DBS. Based on the preliminary results, the best DBS dilution was selected for a final evaluation comparing paired serum and DBS samples from 58 participants. The sensitivity and specificity of the CHIKV ELISA of DBS compared to sera were 100% (95% C.I.: 85.8-100%) and 100% (95% C.I.: 93-100%), respectively. In the linear regression analysis, a coefficient of determination (R 2 ) value of 0.98 indicated the excellent performance of DBS in predicting the serum levels of IgG CHIKV antibodies. Our findings suggest that DBS at an optimized dilution is reliable for investigating the prevalence of CHIKV IgG antibodies during population surveys in the commercial assay tested here. Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interest. (Copyright © 2023. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Chikungunya virus; Dried blood spot; IgG antibody; Validation |
| المشرفين على المادة: | 0 (Antibodies, Viral) 0 (Immunoglobulin G) |
| تواريخ الأحداث: | Date Created: 20230924 Date Completed: 20231030 Latest Revision: 20231031 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.jim.2023.113571 |
| PMID: | 37742825 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7905 |
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| DOI: | 10.1016/j.jim.2023.113571 |