Protein phosphatase 1 regulates core PCP signaling.
| العنوان: | Protein phosphatase 1 regulates core PCP signaling. |
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| المؤلفون: | Song S; Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA.; Present Address: GenScript, 860 Centennial Avenue, Piscataway, NJ, 08854, USA., Cho B; Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA., Weiner AT; Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA., Nissen SB; Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA.; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), University of Copenhagen, Blegdamsvej 3B, DK-2200 Copenhagen N, Denmark., Naharros IO; Department of Ophthalmology, University of California, San Francisco, San Francisco, CA 94143-3120, USA., Bosch PS; Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA., Suyama K; Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA., Hu Y; Department of Genetics, Blavatnik Institute, Harvard Medical School, Harvard University, Boston, MA 02115, USA., He L; Department of Genetics, Blavatnik Institute, Harvard Medical School, Harvard University, Boston, MA 02115, USA.; Present Address: School of Life Sciences, University of Science and Technology of China, Hefei 230027, China., Svinkina T; Broad Institute of MIT and Harvard, Cambridge, MA 02142., Udeshi ND; Broad Institute of MIT and Harvard, Cambridge, MA 02142., Carr SA; Broad Institute of MIT and Harvard, Cambridge, MA 02142., Perrimon N; Department of Genetics, Blavatnik Institute, Harvard Medical School, Harvard University, Boston, MA 02115, USA.; Howard Hughes Medical Institute, Boston, MA 02138, USA., Axelrod JD; Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA. |
| المصدر: | BioRxiv : the preprint server for biology [bioRxiv] 2023 Sep 13. Date of Electronic Publication: 2023 Sep 13. |
| نوع المنشور: | Preprint |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: | PCP signaling polarizes epithelial cells within the plane of an epithelium. Core PCP signaling components adopt asymmetric subcellular localizations within cells to both polarize and coordinate polarity between cells. Achieving subcellular asymmetry requires additional effectors, including some mediating post-translational modifications of core components. Identification of such proteins is challenging due to pleiotropy. We used mass spectrometry-based proximity labeling proteomics to identify such regulators in the Drosophila wing. We identified the catalytic subunit of Protein Phosphatase1, Pp1-87B, and show that it regulates core protein polarization. Pp1-87B interacts with the core protein Van Gogh and at least one Serine/Threonine kinase, Dco/CKIε, that is known to regulate PCP. Pp1-87B modulates Van Gogh subcellular localization and directs its dephosphorylation in vivo. PNUTS, a Pp1 regulatory subunit, also modulates PCP. While the direct substrate(s) of Pp1-87B in control of PCP is not known, our data support the model that cycling between phosphorylated and unphosphorylated forms of one or more core PCP components may regulate acquisition of asymmetry. Finally, our screen serves as a resource for identifying additional regulators of PCP signaling. Competing Interests: Conflict of interest The authors report no conflicts of interest. |
| التعليقات: | Update in: EMBO Rep. 2023 Nov 17;:e56997. (PMID: 37975164) |
| معلومات مُعتمدة: | R01 DK121409 United States DK NIDDK NIH HHS; R35 GM131914 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: APEX proximity labeling; Planar cell polarity; Pp1-87B protein phosphatase; Van gogh |
| تواريخ الأحداث: | Date Created: 20230925 Latest Revision: 20231207 |
| رمز التحديث: | 20231207 |
| مُعرف محوري في PubMed: | PMC10515792 |
| DOI: | 10.1101/2023.09.12.556998 |
| PMID: | 37745534 |
| قاعدة البيانات: | MEDLINE |
| DOI: | 10.1101/2023.09.12.556998 |
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