دورية أكاديمية
Standardization, validation, and comparative evaluation of a faster and high-performance test for quantification of yellow fever neutralizing antibodies.
| العنوان: | Standardization, validation, and comparative evaluation of a faster and high-performance test for quantification of yellow fever neutralizing antibodies. |
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| المؤلفون: | Simões M; Laboratório de Tecnologia Virológica, Instituto de Tecnologia em Imunobiológicos, Fiocruz, Rio de Janeiro, RJ, Brazil. Electronic address: marisol.simoes@bio.fiocruz.br., da Silva SA; Laboratório de Tecnologia Virológica, Instituto de Tecnologia em Imunobiológicos, Fiocruz, Rio de Janeiro, RJ, Brazil., Lúcio KA; Laboratório de Tecnologia Virológica, Instituto de Tecnologia em Imunobiológicos, Fiocruz, Rio de Janeiro, RJ, Brazil., de Oliveira Vieira R; Laboratório de Tecnologia Virológica, Instituto de Tecnologia em Imunobiológicos, Fiocruz, Rio de Janeiro, RJ, Brazil., Schwarcz WD; Laboratório de Tecnologia Virológica, Instituto de Tecnologia em Imunobiológicos, Fiocruz, Rio de Janeiro, RJ, Brazil., de Lima SMB; Laboratório de Tecnologia Virológica, Instituto de Tecnologia em Imunobiológicos, Fiocruz, Rio de Janeiro, RJ, Brazil., Camacho LAB; Escola Nacional de Saúde Pública, Fiocruz, Rio de Janeiro, RJ, Brazil. |
| المصدر: | Journal of immunological methods [J Immunol Methods] 2023 Nov; Vol. 522, pp. 113568. Date of Electronic Publication: 2023 Sep 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 1305440 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7905 (Electronic) Linking ISSN: 00221759 NLM ISO Abbreviation: J Immunol Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Amsterdam, North-Holand, |
| مواضيع طبية MeSH: | Antibodies, Neutralizing* , Yellow Fever*, Humans ; Neutralization Tests/methods ; Antibodies, Viral ; Reference Standards |
| مستخلص: | Although it is considered the reference for quantification of neutralizing antibodies, classical method of the plaque reduction neutralization test (PRNT) is labor intensive, requires specific equipment and inputs, besides a long time for its finalization, even in the micro-PRNT version (in 96-well plates). It has a higher sample throughput, however the smaller wells make the reading of plaques more difficult. With an immunoenzymatic revelation step and a semi-automated reading, the μFRN-HRP (micro Focus Reduction Neutralization - Horseradish Peroxidase) is a faster and more efficient test for the quantification of YF neutralizing antibodies. This study aimed to standardize, validate, and compare it with the reference method in 6-well plates (PRNT). Once the execution protocol was standardized, precision, accuracy, selectivity, and robustness were evaluated to validate the μFRN-HRP. In addition, 200 sera of vaccinees were processed by the μFRN-HRP and by the micro-PRNT to compare with the reference test, estimating agreement by Intraclass Correlation Coefficient (ICC). The standardization and validation of the μFRN-HRP was carried out successfully. Weak to moderate agreement was observed between μFRN-HRP and PRNT for titers in reciprocal dilution, while the same comparison between the classical tests resulted in a better ICC. However, titers in milli-international units obtained by μFRN-HRP showed a substantial agreement with PRNT, while the agreement between micro-PRNT and PRNT was inferior. Therefore, μFRN-HRP can be used in the confirmation of natural YF infection and immune response to vaccination, replacing the micro-PRNT, gaining agility, while preserving the specificity of the result. (Copyright © 2023. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: FRNT; Neutralizing antibodies; PRNT; Standardization; Validation; Yellow fever virus |
| المشرفين على المادة: | 0 (Antibodies, Neutralizing) 0 (Antibodies, Viral) |
| تواريخ الأحداث: | Date Created: 20230925 Date Completed: 20231030 Latest Revision: 20231031 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.jim.2023.113568 |
| PMID: | 37748728 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7905 |
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| DOI: | 10.1016/j.jim.2023.113568 |