دورية أكاديمية
Flow Cytometric Enumeration of Peripheral Blood CD34 + Cells Predicts Bone Marrow Pathology in Patients with Less Than 1% Blasts by Manual Count.
| العنوان: | Flow Cytometric Enumeration of Peripheral Blood CD34 + Cells Predicts Bone Marrow Pathology in Patients with Less Than 1% Blasts by Manual Count. |
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| المؤلفون: | Jelic TM; Department of Pathology and Laboratory Medicine, Charleston Area Medical Center, Charleston, WV, USA., Estalilla OC; Department of Pathology and Laboratory Medicine, Charleston Area Medical Center, Charleston, WV, USA., Vos JA; Department of Pathology, Anatomy and Laboratory Medicine, West Virginia University School of Medicine, Morgantown, WV, USA., Harvey G; Department of Pathology and Laboratory Medicine, Charleston Area Medical Center, Charleston, WV, USA., Stricker CJ; Department of Pathology and Laboratory Medicine, Charleston Area Medical Center, Charleston, WV, USA., Adelanwa AO; Department of Pathology, Anatomy and Laboratory Medicine, West Virginia University School of Medicine, Morgantown, WV, USA., Khalid AA; Cancer Center, Charleston Area Medical Center, Charleston, WV, USA., Plata MJ; Department of Pathology and Laboratory Medicine, Charleston Area Medical Center, Charleston, WV, USA. |
| المصدر: | Journal of blood medicine [J Blood Med] 2023 Sep 21; Vol. 14, pp. 519-535. Date of Electronic Publication: 2023 Sep 21 (Print Publication: 2023). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Dove Medical Press Country of Publication: New Zealand NLM ID: 101550884 Publication Model: eCollection Cited Medium: Print ISSN: 1179-2736 (Print) Linking ISSN: 11792736 NLM ISO Abbreviation: J Blood Med Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: [Auckland, N.Z.] : Dove Medical Press |
| مستخلص: | Background and Aims: Significance of absolute number of CD34 + cells in the peripheral blood of patients with less than 1% myeloblasts by manual differential count is unknown and our aim is to study its relevance in clinical practice. Methods: We studied 138 peripheral bloods flow cytometric analyses in patients with less than 1% myeloblasts by manual differential, when CD34 + events were present in the gate that encompassed lymphocytes, monocytes, stem cells, and blasts. Results: The average absolute number of CD34 + cells in the peripheral blood was 11 CD34 + cells/µL ranging from less than 1 cell/µL to 147 cells/µL. The average absolute number of CD34 + cells in patients with an abnormal expansive process involving bone marrow (metastases, myelodysplasia, granulomas, marrow infections) or if bone marrow biopsy not performed, presumed expansive marrow process was 25 cells/µL, and in patients without an expansive marrow process (or presumed negative) was 4 cells/µL ( P <0.00007). Cutoff 12 CD34 + cells/μL had 93% positive predictive value for bone marrow involvement by an expansive process and 78% negative predictive value. Conclusion: Flow cytometric testing of the peripheral blood is extremely sensitive method for enumerating CD34 + cells and can detect fewer than one CD34 + cell/µL. The absolute number of CD34 + cells in the peripheral blood is a useful parameter in determining marrow involvement by an expansive process and may provide guidance with respect to the necessity for bone marrow biopsy. Competing Interests: The authors report no conflicts of interest in this work. (© 2023 Jelic et al.) |
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| فهرسة مساهمة: | Keywords: CD34 positive cells; bone marrow biopsy; flow cytometry; myeloblasts; peripheral blood |
| تواريخ الأحداث: | Date Created: 20230927 Latest Revision: 20230928 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC10519426 |
| DOI: | 10.2147/JBM.S417432 |
| PMID: | 37753388 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1179-2736 |
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| DOI: | 10.2147/JBM.S417432 |