دورية أكاديمية
أعرض في EDS

Programmable RNA-guided DNA endonucleases are widespread in eukaryotes and their viruses.

التفاصيل البيبلوغرافية
العنوان: Programmable RNA-guided DNA endonucleases are widespread in eukaryotes and their viruses.
المؤلفون: Jiang K; McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology, Cambridge, MA 02139, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Lim J; McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Sgrizzi S; McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Trinh M; McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Kayabolen A; McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Yutin N; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA., Bao W; Genetic Information Research Institute, 20380 Town Center Ln, Suite 240, Cupertino, CA, USA., Kato K; Structural Biology Division, Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo 153-8904, Japan.; Department of Molecular and Mechanistic Immunology, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8510, Japan., Koonin EV; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA., Gootenberg JS; McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Abudayyeh OO; McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
المصدر: Science advances [Sci Adv] 2023 Sep 29; Vol. 9 (39), pp. eadk0171. Date of Electronic Publication: 2023 Sep 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101653440 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2375-2548 (Electronic) Linking ISSN: 23752548 NLM ISO Abbreviation: Sci Adv Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, DC : American Association for the Advancement of Science, [2015]-
مواضيع طبية MeSH: Eukaryota*/genetics , Eukaryota*/metabolism , Viruses*/genetics, Humans ; Deoxyribonuclease I ; RNA/genetics ; Deoxyribonucleases/metabolism
مستخلص: Programmable RNA-guided DNA nucleases perform numerous roles in prokaryotes, but the extent of their spread outside prokaryotes is unclear. Fanzors, the eukaryotic homolog of prokaryotic TnpB proteins, have been detected in genomes of eukaryotes and large viruses, but their activity and functions in eukaryotes remain unknown. Here, we characterize Fanzors as RNA-programmable DNA endonucleases, using biochemical and cellular evidence. We found diverse Fanzors that frequently associate with various eukaryotic transposases. Reconstruction of Fanzors evolution revealed multiple radiations of RuvC-containing TnpB homologs in eukaryotes. Fanzor genes captured introns and proteins acquired nuclear localization signals, indicating extensive, long-term adaptation to functioning in eukaryotic cells. Fanzor nucleases contain a rearranged catalytic site of the RuvC domain, similar to a distinct subset of TnpBs, and lack collateral cleavage activity. We demonstrate that Fanzors can be harnessed for genome editing in human cells, highlighting the potential of these widespread eukaryotic RNA-guided nucleases for biotechnology applications.
التعليقات: Update of: bioRxiv. 2023 Jun 14;:. (PMID: 37398409)
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معلومات مُعتمدة: R01 AG074932 United States AG NIA NIH HHS; R01 EB031957 United States EB NIBIB NIH HHS; R21 AI149694 United States AI NIAID NIH HHS; R56 HG011857 United States HG NHGRI NIH HHS
المشرفين على المادة: EC 3.1.21.1 (Deoxyribonuclease I)
63231-63-0 (RNA)
EC 3.1.- (Deoxyribonucleases)
تواريخ الأحداث: Date Created: 20230927 Date Completed: 20231004 Latest Revision: 20231019
رمز التحديث: 20231020
مُعرف محوري في PubMed: PMC10530073
DOI: 10.1126/sciadv.adk0171
PMID: 37756409
قاعدة البيانات: MEDLINE
الوصف
تدمد:2375-2548
DOI:10.1126/sciadv.adk0171