دورية أكاديمية
Reexamining the Minimum Sweat Rate Requirement for Sweat Chloride Testing.
| العنوان: | Reexamining the Minimum Sweat Rate Requirement for Sweat Chloride Testing. |
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| المؤلفون: | Omosule CL; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, United States., Dietzen DJ; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, United States.; Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, United States., Roper SM; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, United States.; Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, United States. |
| المصدر: | The journal of applied laboratory medicine [J Appl Lab Med] 2023 Nov 02; Vol. 8 (6), pp. 1084-1091. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 101693884 Publication Model: Print Cited Medium: Print ISSN: 2576-9456 (Print) Linking ISSN: 24757241 NLM ISO Abbreviation: J Appl Lab Med Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2020- : Oxford : Oxford University Press Original Publication: [Washington, DC] : American Association for Clinical Chemistry, [2016]-[2019] |
| مواضيع طبية MeSH: | Sweat*/chemistry , Cystic Fibrosis*/diagnosis, Humans ; Chlorides ; Nonoxynol |
| مستخلص: | Background: Guidelines for sweat chloride testing endorse a minimum sweat rate for reporting results. Bilateral sweat collection is recommended, but if both sites fail to meet the minimum rate (quantity not sufficient, QNS), the test should be repeated. In this study, we examine the correlation between sweat rate and sweat chloride concentration ([Cl-]), assess the accuracy of specimens collected at suboptimal rates, and investigate the use of pooled bilateral specimens for chloride measurement. Methods: Pearson correlation was employed to analyze the relationship between sweat rate and chloride concentration, [Cl-], in 674 macroduct collections. Weighted kappa was evaluated to determine cystic fibrosis (CF) diagnostic classification concordance for 18 tests with paired arms above vs below the minimum sweat rate. Deming regression was applied to compare [Cl-] from pooled bilateral specimens vs neat specimens in 27 collections with residual volume available after clinical testing. Results: Pearson correlation of sweat rate vs [Cl-] was minimal (r = -0.0735) across specimens with varying rates and [Cl-]. There was substantial agreement in CF diagnostic classification between arms for bilateral collections with discordant sweat rates. Regression analysis of [Cl-] in pooled vs nonpooled specimens revealed a slope of 0.984 and an intercept of 0.796. Conclusions: Negligible correlation of sweat rate and [Cl-] suggests the minimum sweat rate for macroduct collectors may be overly stringent. Reporting of [Cl-] in specimens with ≥10 µL (rate ≥0.3 µL/min) may reduce QNS rates without compromising diagnostic accuracy. Preliminary data suggests pooling of bilateral collections may be a feasible option to achieve the required volume for testing. (© Association for Diagnostics & Laboratory Medicine 2023. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| المشرفين على المادة: | 0 (Chlorides) 26027-38-3 (Nonoxynol) |
| تواريخ الأحداث: | Date Created: 20230927 Date Completed: 20231108 Latest Revision: 20231114 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1093/jalm/jfad067 |
| PMID: | 37757465 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2576-9456 |
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| DOI: | 10.1093/jalm/jfad067 |