دورية أكاديمية
أعرض في EDS

Protective Effects of Peroxiredoxin 6 in Pro-Inflammatory Response Model Using Raw 264.7 Macrophages.

التفاصيل البيبلوغرافية
العنوان: Protective Effects of Peroxiredoxin 6 in Pro-Inflammatory Response Model Using Raw 264.7 Macrophages.
المؤلفون: Parfenyuk SB; Institute of Cell Biophysics, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. lana_kras2@rambler.ru., Glushkova OV; Institute of Cell Biophysics, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia., Sharapov MG; Institute of Cell Biophysics, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia., Khrenov MO; Institute of Cell Biophysics, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia., Lunin SM; Institute of Cell Biophysics, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia., Kuzekova AA; Institute of Cell Biophysics, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia., Mubarakshina EK; Institute of Cell Biophysics, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia., Novoselova TV; Institute of Cell Biophysics, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia., Cherenkov DA; Voronezh State University of Engineering Technologies, Voronezh, 394036, Russia., Novoselova EG; Institute of Cell Biophysics, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.
المصدر: Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2023 Aug; Vol. 88 (8), pp. 1156-1164.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MAIK Nauka/Interperiodica Country of Publication: United States NLM ID: 0376536 Publication Model: Print Cited Medium: Internet ISSN: 1608-3040 (Electronic) Linking ISSN: 00062979 NLM ISO Abbreviation: Biochemistry (Mosc) Subsets: MEDLINE
أسماء مطبوعة: Publication: <2007->: Moscow : MAIK Nauka/Interperiodica
Original Publication: New York, Consultants Bureau [etc.]
مواضيع طبية MeSH: Inflammation*/chemically induced , Inflammation*/metabolism , Macrophages*/metabolism , Peroxiredoxin VI*/genetics, Animals ; Mice ; Cytokines/metabolism ; Lipopolysaccharides/pharmacology ; Lipopolysaccharides/metabolism ; NF-kappa B/metabolism ; RAW 264.7 Cells ; Signal Transduction
مستخلص: The aim of the work was to study effects of peroxiredoxin 6 (PRDX6), a recombinant antioxidant protein, on the level of pro-inflammatory responses of RAW 264.7 macrophages to endotoxin exposure. Addition of LPS to the RAW 264.7 cell culture medium expectedly increased production of TNF-α, and addition of PRDX6 led to a significant (15-20%) decrease in its production. The level of production of another pro-inflammatory cytokine, IL-1β, which was significantly activated by endotoxin, was completely normalized under the PRDX6 action. Moreover, addition of PRDX6 reduced production of reactive oxygen species (ROS) induced by endotoxin and also prevented overexpression of the iNos gene in the RAW 264.7 cells. The results showed that PRDX6 had a suppressive effect on the expression of Nrf-2 gene and production of the transcription factor NRF-2 during the first 6 h of cell cultivation. Addition of endotoxin caused activation of the NF-κB and SAPK/JNK signaling cascades, while in the presence of PRDX6, activity of these signaling cascades decreases. It is known that the pro-inflammatory response of cells caused by exposure to bacterial LPS leads to activation of apoptosis and elimination of the damaged cells. Our studies confirm this, since exposure to LPS led to activation of the expression of P53 gene, a marker of apoptosis. Peroxiredoxin 6 added within the first hours of the development of acute pro-inflammatory response suppressed the P53 gene expression, indicating protective effect of PRDX6 that reduced apoptosis in the RAW 264.7 macrophages.
فهرسة مساهمة: Keywords: cytokines; gene expression; inflammation; peroxiredoxin 6; signaling cascades
المشرفين على المادة: 0 (Cytokines)
0 (Lipopolysaccharides)
0 (NF-kappa B)
EC 1.11.1.15 (Peroxiredoxin VI)
تواريخ الأحداث: Date Created: 20230927 Date Completed: 20231003 Latest Revision: 20231003
رمز التحديث: 20231215
DOI: 10.1134/S0006297923080096
PMID: 37758314
قاعدة البيانات: MEDLINE
الوصف
تدمد:1608-3040
DOI:10.1134/S0006297923080096