دورية أكاديمية
Unraveling gene expression and genetic instability in dental fluorosis: Investigating the impact of chronic fluoride exposure.
| العنوان: | Unraveling gene expression and genetic instability in dental fluorosis: Investigating the impact of chronic fluoride exposure. |
|---|---|
| المؤلفون: | Garcia ALH; Laboratory of Genetic Toxicology, PPGBioSaúde (Postgraduate Program in Cellular and Molecular Biology Applied to Health), Lutheran University of Brazil (ULBRA), 92425-900 Canoas, Rio Grande do Sul, Brazil; Laboratory of Genetic Toxicology, La Salle University (UniLaSalle), Canoas, Rio Grande do Sul, Brazil. Electronic address: analeticiagarcia82@gmail.com., de Souza MR; Laboratory of Genetic Toxicology, PPGBioSaúde (Postgraduate Program in Cellular and Molecular Biology Applied to Health), Lutheran University of Brazil (ULBRA), 92425-900 Canoas, Rio Grande do Sul, Brazil., Picinini J; Laboratory of Genetic Toxicology, PPGBioSaúde (Postgraduate Program in Cellular and Molecular Biology Applied to Health), Lutheran University of Brazil (ULBRA), 92425-900 Canoas, Rio Grande do Sul, Brazil., Soares S; Laboratory of Genetic Toxicology, PPGBioSaúde (Postgraduate Program in Cellular and Molecular Biology Applied to Health), Lutheran University of Brazil (ULBRA), 92425-900 Canoas, Rio Grande do Sul, Brazil., Rohr P; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, São Paulo, Brazil., Linden R; Laboratory of Analytical Toxicology, Institute of Health Sciences, Graduate Program on Toxicology and Analytical Toxicology, Feevale University, Novo Hamburgo, RS, Brazil., Schneider A; Laboratory of Analytical Toxicology, Institute of Health Sciences, Graduate Program on Toxicology and Analytical Toxicology, Feevale University, Novo Hamburgo, RS, Brazil., Freitas MPM; Lutheran University of Brazil (ULBRA), Graduate Program in Dentistry, 92425-900 Canoas, Rio Grande do Sul, Brazil., Ely HC; Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil., Bobermin LD; Departamento de Bioquímica, Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2600, Anexo, Bairro Santa Cecília, Porto Alegre, RS 90035-003, Brazil., Dos Santos AQ; Departamento de Bioquímica, Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2600, Anexo, Bairro Santa Cecília, Porto Alegre, RS 90035-003, Brazil., Dalberto D; Laboratory of Genetic Toxicology, PPGBioSaúde (Postgraduate Program in Cellular and Molecular Biology Applied to Health), Lutheran University of Brazil (ULBRA), 92425-900 Canoas, Rio Grande do Sul, Brazil., da Silva J; Laboratory of Genetic Toxicology, PPGBioSaúde (Postgraduate Program in Cellular and Molecular Biology Applied to Health), Lutheran University of Brazil (ULBRA), 92425-900 Canoas, Rio Grande do Sul, Brazil; Laboratory of Genetic Toxicology, La Salle University (UniLaSalle), Canoas, Rio Grande do Sul, Brazil. Electronic address: juliana.silva@ulbra.br. |
| المصدر: | The Science of the total environment [Sci Total Environ] 2024 Jan 01; Vol. 906, pp. 167393. Date of Electronic Publication: 2023 Sep 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0330500 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1026 (Electronic) Linking ISSN: 00489697 NLM ISO Abbreviation: Sci Total Environ Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier. |
| مواضيع طبية MeSH: | Fluorides*/toxicity , Fluorosis, Dental*/epidemiology, Humans ; DNA ; Genomic Instability ; Gene Expression ; Prevalence |
| مستخلص: | Chronic fluoride exposure, even in small quantities, when continuously ingested by the human population, can lead to a significant public health concern known as fluorosis. Our understanding of the effects of fluoride on human health, as well as its potential to impact DNA, is limited. The present study aimed to assess genetic instability in 20 individuals diagnosed with dental fluorosis and 20 individuals without the condition from the state of Rio Grande do Sul, Brazil. The participants' dental fluorosis was evaluated using the Thylstrup-Fejerskov index (TF). To further evaluate genetic instability, several assays were conducted, including the alkaline and modified (+FPG) comet assay (using a visual score, VS), the buccal micronucleus (MN) cytome (BMCyt) assay, the cytokinesis-block MN (CBMN-Cyt) assay, and the measurement of telomere length (TL). In addition, the study utilized tools from Systems Biology to gain insights into the effects of fluoride exposure on humans, which aided in the selection and evaluation of mRNA expression levels of specific genes, namely PPA1 (inorganic pyrophosphatase 1), AQP5 (Aquaporin 5), and MT-ATP6 (Mitochondrially Encoded Adenosine Triphosphate Synthase Membrane Subunit 6). Furthermore, fluoride levels in the blood and urine were assessed using an ion-selective electrode, along with the evaluation of the inflammatory response in serum. The group with dental fluorosis exhibited 2.18 times higher MN frequencies specifically when assessed using the CBMN-Cyt assay, in comparison with individuals without fluorosis. Findings from the enzyme-modified comet assay indicated oxidative damage to purines in DNA. Furthermore, a decrease in TL was observed, along with elevated expression patterns of the PPA1 and AQP5 genes, and significant alterations in cytokine release. Significant correlations were identified between the TF and age, as well as the levels of necrotic cells. Additionally, noteworthy correlations were established between fluoride levels and the levels of MN, VS, and MT-ATP6. Although dental fluorosis results from fluoride exposure, our research highlights the potential influence of this condition on genomic instability and gene expression. Consequently, our findings stress the importance of continuously monitoring populations with a high incidence of dental fluorosis to enhance our comprehension of how genomic instability might correlate with the origins and consequences of health problems in these individuals. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Dental fluorosis; Gene expression; Micronucleus; Mutagenicity tests; System biology; Telomere |
| المشرفين على المادة: | Q80VPU408O (Fluorides) 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20230928 Date Completed: 20231115 Latest Revision: 20231115 |
| رمز التحديث: | 20231115 |
| DOI: | 10.1016/j.scitotenv.2023.167393 |
| PMID: | 37769727 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-1026 |
|---|---|
| DOI: | 10.1016/j.scitotenv.2023.167393 |