دورية أكاديمية
أعرض في EDS

3D printing-based frugal manufacturing of glass pipettes for minimally invasive delivery of therapeutics to the brain.

التفاصيل البيبلوغرافية
العنوان: 3D printing-based frugal manufacturing of glass pipettes for minimally invasive delivery of therapeutics to the brain.
المؤلفون: Qiao G; Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA., Gulisashvili D; Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA., Jablonska A; Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA., Zhao G; Laboratory of Molecular Cardiology, Department of Physiology, Center for Biomedical Engineering and Technology, University of Maryland School of Medicine, Baltimore, Maryland, USA., Janowski M; Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA., Walczak P; Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA., Liang Y; Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA.
المصدر: Neuroprotection [Neuroprotection] 2023 Sep; Vol. 1 (1), pp. 58-65. Date of Electronic Publication: 2023 Jun 19.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: John Wiley & Sons Limited Country of Publication: England NLM ID: 9918452086806676 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2770-730X (Electronic) Linking ISSN: 27707296 NLM ISO Abbreviation: Neuroprotection Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Chichester : John Wiley & Sons Limited, 2022-
مستخلص: Objective: Intracerebral delivery of agents in liquid form is usually achieved through commercially available and durable metal needles. However, their size and texture may contribute to mechanical brain damage. Glass pipettes with a thin tip may significantly reduce injection-associated brain damage but require access to prohibitively expensive programmable pipette pullers. This study is to remove the economic barrier to the application of minimally invasive delivery of therapeutics to the brain, such as chemical compounds, viral vectors, and cells.
Methods: We took advantage of the rapid development of free educational online resources and emerging low-cost 3D printers by designing an affordable pipette puller (APP) to remove the cost obstacle.
Results: We showed that our APP could produce glass pipettes with a sharp tip opening down to 20 μm or less, which is sufficiently thin for the delivery of therapeutics into the brain. A pipeline from pipette pulling to brain injection using low-cost and open-source equipment was established to facilitate the application of the APP.
Conclusion: In the spirit of frugal science, our device may democratize glass pipette-puling and substantially promote the application of minimally invasive and precisely controlled delivery of therapeutics to the brain for finding more effective therapies of brain diseases.
Competing Interests: CONFLICT OF INTEREST STATEMENT P. W. and Y. L are members of the Neuroprotection Editorial Board and are not involved in the peer review process of this article.
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معلومات مُعتمدة: R03 NS123733 United States NS NINDS NIH HHS; R03 NS128459 United States NS NINDS NIH HHS; R21 AG077631 United States AG NIA NIH HHS
فهرسة مساهمة: Keywords: 3D-printable; brain injection; frugal science; glass pipette pulling
تواريخ الأحداث: Date Created: 20230929 Latest Revision: 20240210
رمز التحديث: 20240210
مُعرف محوري في PubMed: PMC10538625
DOI: 10.1002/nep3.20
PMID: 37771648
قاعدة البيانات: MEDLINE
الوصف
تدمد:2770-730X
DOI:10.1002/nep3.20