دورية أكاديمية
Zoonotic tuberculosis in a high bovine tuberculosis burden area of Ethiopia.
| العنوان: | Zoonotic tuberculosis in a high bovine tuberculosis burden area of Ethiopia. |
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| المؤلفون: | Ayalew S; Armauer Hansen Research Institute, Addis Ababa, Ethiopia.; Department of Biology, College of Natural Sciences, Arba Minch University, Arba Minch, Ethiopia., Habtamu G; Armauer Hansen Research Institute, Addis Ababa, Ethiopia., Melese F; Armauer Hansen Research Institute, Addis Ababa, Ethiopia., Tessema B; Armauer Hansen Research Institute, Addis Ababa, Ethiopia., Ashford RT; Department of Bacteriology, Animal and Plant Health Agency, Weybridge, United Kingdom., Chothe SK; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, State College, PA, United States., Aseffa A; Armauer Hansen Research Institute, Addis Ababa, Ethiopia., Wood JLN; Disease Dynamics Unit, Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom., Berg S; Department of Bacteriology, Animal and Plant Health Agency, Weybridge, United Kingdom.; Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany., Mihret A; Armauer Hansen Research Institute, Addis Ababa, Ethiopia. |
| مؤلفون مشاركون: | ETHICOBOTS Consortium |
| المصدر: | Frontiers in public health [Front Public Health] 2023 Sep 13; Vol. 11, pp. 1204525. Date of Electronic Publication: 2023 Sep 13 (Print Publication: 2023). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Editorial Office Country of Publication: Switzerland NLM ID: 101616579 Publication Model: eCollection Cited Medium: Internet ISSN: 2296-2565 (Electronic) Linking ISSN: 22962565 NLM ISO Abbreviation: Front Public Health Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Editorial Office |
| مواضيع طبية MeSH: | Tuberculosis, Bovine*/epidemiology , Tuberculosis, Bovine*/diagnosis , Tuberculosis, Bovine*/microbiology , Mycobacterium bovis* , Mycobacterium tuberculosis*/genetics , Tuberculosis, Lymph Node*/epidemiology , Tuberculosis, Lymph Node*/veterinary , Tuberculosis, Lymph Node*/microbiology, Animals ; Cattle ; Humans ; Ethiopia/epidemiology ; Cross-Sectional Studies |
| مستخلص: | Background: Tuberculosis (TB) is a major cause of ill health and one of the leading causes of death worldwide, caused by species of the Mycobacterium tuberculosis complex (MTBC), with Mycobacterium tuberculosis being the dominant pathogen in humans and Mycobacterium bovis in cattle. Zoonotic transmission of TB (zTB) to humans is frequent particularly where TB prevalence is high in cattle. In this study, we explored the prevalence of zTB in central Ethiopia, an area highly affected by bovine TB (bTB) in cattle. Method: A convenient sample of 385 patients with pulmonary tuberculosis (PTB, N = 287) and tuberculous lymphadenitis (TBLN, N = 98) were included in this cross-sectional study in central Ethiopia. Sputum and fine needle aspirate (FNA) samples were obtained from patients with PTB and TBLN, respectively, and cultures were performed using BACTEC ™ MGIT ™ 960. All culture positive samples were subjected to quantitative PCR (qPCR) assays, targeting IS 1081 , RD9 and RD4 genomic regions for detection of MTBC, M. tuberculosis and M. bovis , respectively. Results: Two hundred and fifty-five out of 385 sampled patients were culture positive and all were isolates identified as MTBC by being positive for the IS 1081 assay. Among them, 249 (97.6%) samples had also a positive RD9 result (intact RD9 locus) and were consequently classified as M. tuberculosis . The remaining six (2.4%) isolates were RD4 deficient and thereby classified as M. bovis . Five out of these six M. bovis strains originated from PTB patients whereas one was isolated from a TBLN patient. Occupational risk and the widespread consumption of raw animal products were identified as potential sources of M. bovis infection in humans, and the isolation of M. bovis from PTB patients suggests the possibility of human-to-human transmission, particularly in patients with no known contact history with animals. Conclusion: The detected proportion of culture positive cases of 2.4% being M. bovis from this region was higher zTB rate than previously reported for the general population of Ethiopia. Patients with M. bovis infection are more likely to get less efficient TB treatment because M. bovis is inherently resistant to pyrazinamide. MTBC species identification should be performed where M. bovis is common in cattle, especially in patients who have a history of recurrence or treatment failure. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Ayalew, Habtamu, Melese, Tessema, Ashford, Chothe, Aseffa, Wood, Berg, Mihret and The ETHICOBOTS Consortium.) |
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| معلومات مُعتمدة: | D43 TW009127 United States TW FIC NIH HHS; United Kingdom MRC_ Medical Research Council; United Kingdom BB_ Biotechnology and Biological Sciences Research Council |
| فهرسة مساهمة: | Investigator: A Aseffa; A Mihret; B Tessema; B Belachew; E Fekadu; F Melese; G Gemechu; H Taye; R Tschopp; S Haile; S Ayalew; T Hailu; R Tschopp; A Bekele; C Yirga; M Ambaw; T Mamo; T Solomon; T Teklewold; S Gebre; G Gari; A Aliy; A Olani; A Sirak; G Almaw; G Mekonnen; M Tamiru; S Guta; J Wood; A Conlan; A Clarke; HL Moore; C Hodge; RG Hewinson; S Berg; M Vordermeier; J Nunez-Garcia; G Ameni; B Bayissa; A Zewude; A Worku; L Terfassa; M Chanyalew; T Mohammed; M Zeleke Keywords: Mycobacterium bovis; Mycobacterium tuberculosis; cattle; central Ethiopia; zoonosis |
| تواريخ الأحداث: | Date Created: 20230929 Date Completed: 20231023 Latest Revision: 20240530 |
| رمز التحديث: | 20240530 |
| مُعرف محوري في PubMed: | PMC10525399 |
| DOI: | 10.3389/fpubh.2023.1204525 |
| PMID: | 37771833 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2296-2565 |
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| DOI: | 10.3389/fpubh.2023.1204525 |