دورية أكاديمية
أعرض في EDS

SRSF1-mediated alternative splicing is required for spermatogenesis.

التفاصيل البيبلوغرافية
العنوان: SRSF1-mediated alternative splicing is required for spermatogenesis.
المؤلفون: Lei WL; Guangdong and Shenzhen Key Laboratory of Reproductive Medicine and Genetics, The Center of Reproductive Medicine, Peking University Shenzhen Hospital, Shenzhen 518000, China.; Department of Urology & Andrology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China., Li YY; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China., Du Z; School of Artificial Intelligence, University of Chinese Academy of Sciences, Beijing, 100049, China., Su R; Guangzhou Key Laboratory of Metabolic Diseases and Reproductive Health, Guangdong-Hongkong Metabolism & Reproduction Joint Laboratory, Reproductive Medicine Center, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China., Meng TG; Guangzhou Key Laboratory of Metabolic Diseases and Reproductive Health, Guangdong-Hongkong Metabolism & Reproduction Joint Laboratory, Reproductive Medicine Center, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China., Ning Y; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China., Hou G; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China., Schatten H; Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65211, USA., Wang ZB; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China., Han Z; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China., Sun F; Department of Urology & Andrology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China., Qian WP; Guangdong and Shenzhen Key Laboratory of Reproductive Medicine and Genetics, The Center of Reproductive Medicine, Peking University Shenzhen Hospital, Shenzhen 518000, China., Liu C; CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China., Sun QY; Guangzhou Key Laboratory of Metabolic Diseases and Reproductive Health, Guangdong-Hongkong Metabolism & Reproduction Joint Laboratory, Reproductive Medicine Center, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China.
المصدر: International journal of biological sciences [Int J Biol Sci] 2023 Sep 11; Vol. 19 (15), pp. 4883-4897. Date of Electronic Publication: 2023 Sep 11 (Print Publication: 2023).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Ivyspring International Country of Publication: Australia NLM ID: 101235568 Publication Model: eCollection Cited Medium: Internet ISSN: 1449-2288 (Electronic) Linking ISSN: 14492288 NLM ISO Abbreviation: Int J Biol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Lake Haven, N.S.W., Australia : Ivyspring International, c2004-
مواضيع طبية MeSH: Alternative Splicing*/genetics , Spermatogenesis*/genetics, Male ; Serine-Arginine Splicing Factors/genetics ; Serine-Arginine Splicing Factors/metabolism ; Testis/metabolism ; Animals
مستخلص: Alternative splicing (AS) plays significant roles in a multitude of fundamental biological activities. AS is prevalent in the testis, but the regulations of AS in spermatogenesis is only little explored. Here, we report that Serine/arginine-rich splicing factor 1 (SRSF1) plays critical roles in alternative splicing and male reproduction. Male germ cell-specific deletion of Srsf1 led to complete infertility by affecting spermatogenesis. Mechanistically, by combining RNA-seq data with LACE-seq data, we showed that SRSF1 affected the AS of Stra8 in a direct manner and Dazl , Dmc1 , Mre11a , Syce2 and Rif1 in an indirect manner. Our findings demonstrate that SRSF1 has crucial functions in spermatogenesis and male fertility by regulating alternative splicing.
Competing Interests: Competing Interests: The authors have declared that no competing interest exists.
(© The author(s).)
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فهرسة مساهمة: Keywords: SRSF1; alternative splicing; male infertility; spermatogenesis
المشرفين على المادة: 170974-22-8 (Serine-Arginine Splicing Factors)
تواريخ الأحداث: Date Created: 20231002 Date Completed: 20231005 Latest Revision: 20231005
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10539708
DOI: 10.7150/ijbs.83474
PMID: 37781512
قاعدة البيانات: MEDLINE
الوصف
تدمد:1449-2288
DOI:10.7150/ijbs.83474