دورية أكاديمية
SRSF1-mediated alternative splicing is required for spermatogenesis.
العنوان: | SRSF1-mediated alternative splicing is required for spermatogenesis. |
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المؤلفون: | Lei WL; Guangdong and Shenzhen Key Laboratory of Reproductive Medicine and Genetics, The Center of Reproductive Medicine, Peking University Shenzhen Hospital, Shenzhen 518000, China.; Department of Urology & Andrology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China., Li YY; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China., Du Z; School of Artificial Intelligence, University of Chinese Academy of Sciences, Beijing, 100049, China., Su R; Guangzhou Key Laboratory of Metabolic Diseases and Reproductive Health, Guangdong-Hongkong Metabolism & Reproduction Joint Laboratory, Reproductive Medicine Center, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China., Meng TG; Guangzhou Key Laboratory of Metabolic Diseases and Reproductive Health, Guangdong-Hongkong Metabolism & Reproduction Joint Laboratory, Reproductive Medicine Center, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China., Ning Y; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China., Hou G; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China., Schatten H; Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65211, USA., Wang ZB; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China., Han Z; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China., Sun F; Department of Urology & Andrology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China., Qian WP; Guangdong and Shenzhen Key Laboratory of Reproductive Medicine and Genetics, The Center of Reproductive Medicine, Peking University Shenzhen Hospital, Shenzhen 518000, China., Liu C; CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China., Sun QY; Guangzhou Key Laboratory of Metabolic Diseases and Reproductive Health, Guangdong-Hongkong Metabolism & Reproduction Joint Laboratory, Reproductive Medicine Center, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China. |
المصدر: | International journal of biological sciences [Int J Biol Sci] 2023 Sep 11; Vol. 19 (15), pp. 4883-4897. Date of Electronic Publication: 2023 Sep 11 (Print Publication: 2023). |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Ivyspring International Country of Publication: Australia NLM ID: 101235568 Publication Model: eCollection Cited Medium: Internet ISSN: 1449-2288 (Electronic) Linking ISSN: 14492288 NLM ISO Abbreviation: Int J Biol Sci Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Lake Haven, N.S.W., Australia : Ivyspring International, c2004- |
مواضيع طبية MeSH: | Alternative Splicing*/genetics , Spermatogenesis*/genetics, Male ; Serine-Arginine Splicing Factors/genetics ; Serine-Arginine Splicing Factors/metabolism ; Testis/metabolism ; Animals |
مستخلص: | Alternative splicing (AS) plays significant roles in a multitude of fundamental biological activities. AS is prevalent in the testis, but the regulations of AS in spermatogenesis is only little explored. Here, we report that Serine/arginine-rich splicing factor 1 (SRSF1) plays critical roles in alternative splicing and male reproduction. Male germ cell-specific deletion of Srsf1 led to complete infertility by affecting spermatogenesis. Mechanistically, by combining RNA-seq data with LACE-seq data, we showed that SRSF1 affected the AS of Stra8 in a direct manner and Dazl , Dmc1 , Mre11a , Syce2 and Rif1 in an indirect manner. Our findings demonstrate that SRSF1 has crucial functions in spermatogenesis and male fertility by regulating alternative splicing. Competing Interests: Competing Interests: The authors have declared that no competing interest exists. (© The author(s).) |
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فهرسة مساهمة: | Keywords: SRSF1; alternative splicing; male infertility; spermatogenesis |
المشرفين على المادة: | 170974-22-8 (Serine-Arginine Splicing Factors) |
تواريخ الأحداث: | Date Created: 20231002 Date Completed: 20231005 Latest Revision: 20231005 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC10539708 |
DOI: | 10.7150/ijbs.83474 |
PMID: | 37781512 |
قاعدة البيانات: | MEDLINE |
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