دورية أكاديمية
Prognostic utility of MicroRNA-221 and interleukin-6 in cerebral ischemic stroke.
| العنوان: | Prognostic utility of MicroRNA-221 and interleukin-6 in cerebral ischemic stroke. |
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| المؤلفون: | Emam WA; Department of Biochemistry, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt., Ibrahim NE; Department of Microbial Biotechnology, Biotechnology Research Institute, National Research Centre, Dokki, Giza, Egypt., El-Senosy FM; Department of Internal Medicine, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt., Elsheikh AA; Department of Community and Occupational Medicine, Faculty of Medicine (for Girls), Al-Azhar University., Elsayed AE; Department of Neurology, StrokeeUnit, El-Koba Military Hospital, Cairo Egypt., El Attar R; Department of Neurology, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt., Elhadad SM; Department of Internal Medicine, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt., Alhabibi AM; Department of Clinical Pathology, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt., Mohammed AR; Department of Biochemistry, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt. |
| المصدر: | The Egyptian journal of immunology [Egypt J Immunol] 2023 Oct; Vol. 30 (4), pp. 11-20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Modern Commercial Press Country of Publication: Egypt NLM ID: 9816016 Publication Model: Print Cited Medium: Print ISSN: 1110-4902 (Print) NLM ISO Abbreviation: Egypt J Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cairo : Modern Commercial Press, |
| مواضيع طبية MeSH: | Ischemic Stroke*/diagnosis , Ischemic Stroke*/genetics , Stroke*/diagnosis , Stroke*/genetics , MicroRNAs*/genetics, Humans ; Interleukin-6 ; Prognosis |
| مستخلص: | Cerebral ischemic stroke has a significant mortality rate and persistent impairment. The initial diagnosis of stroke occurs by magnetic resonance imaging and computed tomography. There is a strong need for more accessible, less expensive, and non-invasive methods besides the neuroimaging methods. MicroRNAs (miRNAs) are critical regulators for ischemic stroke as they are involved in stroke pathophysiology. The goal of the current study was to determine whether microRNA-221 (miR-221) could be used as a diagnostic biomarker for patients with ischemic stroke, and whether it can serve as a promising indicator of the disease severity especially if combined with interleukin-6 (IL-6). The study included 90 subjects, 45 cerebral ischemic stroke patients and 45 controls. MiR-221 was evaluated by quantitative real-time polymerase chain reaction (q-PCR) and IL-6 by enzyme-linked immunosorbent assay (ELISA). Our study results revealed that the serum miR-221 level was significantly reduced in cerebral ischemic stroke patients when compared to the control group (p<0.0001). In addition, serum miR-221 showed a significant negative correlation with cerebral stroke severity (p<0.0001), whereas serum IL-6 showed a significant positive correlation with cerebral stroke severity (p < 0.0001). We also analyzed the receiver operator characteristic (ROC) curve and found that area under the ROC curve (AUC) for severity of ischemic stroke by miR-221 was 0.97 (95% confidence intervall0.93-1, p<0.001). Notably, the combination of serum miR-221 with IL-6 for prediction of ischemic stroke severity showed both increased sensitivity/specificity (AUC=0.99, 95% confidence interval 0.96-1, p<0.001) than miR-221 alone. We concluded that miR-221 constituted a non-invasive, sensitive, and specific biomarker that could be used for diagnosis of ischemic stroke and for prediction of its severity. (Copyright© by the Egyptian Association of Immunologists.) |
| المشرفين على المادة: | 0 (Interleukin-6) 0 (MicroRNAs) 0 (MIRN221 microRNA, human) |
| تواريخ الأحداث: | Date Created: 20231003 Date Completed: 20231004 Latest Revision: 20231004 |
| رمز التحديث: | 20231215 |
| PMID: | 37787681 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1110-4902 |
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