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Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture.

التفاصيل البيبلوغرافية
العنوان: Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture.
المؤلفون: 'Aizat Norhisham D; Functional Omics and Bioprocess Development Laboratory, Institute of Biological Sciences, Faculty of Science, Universiti Malaya, Kuala Lumpur, Malaysia.; Agro-Biotechnology Institute, National Institutes of Biotechnology Malaysia, Serdang, Malaysia.; Zhejiang Provincial Key Laboratory for Subtropical Water Environment and Marine Biological Resources Protection, Wenzhou University, Wenzhou, China.; Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Semenyih, Malaysia.; Department of Sustainable Engineering, Saveetha School of Engineering, SIMATS, Chennai, India., Md Saad N; Agro-Biotechnology Institute, National Institutes of Biotechnology Malaysia, Serdang, Malaysia., Ahmad Usuldin SR; Functional Omics and Bioprocess Development Laboratory, Institute of Biological Sciences, Faculty of Science, Universiti Malaya, Kuala Lumpur, Malaysia.; Agro-Biotechnology Institute, National Institutes of Biotechnology Malaysia, Serdang, Malaysia., Vayabari DAG; Functional Omics and Bioprocess Development Laboratory, Institute of Biological Sciences, Faculty of Science, Universiti Malaya, Kuala Lumpur, Malaysia., Ilham Z; Environmental Science and Management Program, Institute of Biological Sciences, Faculty of Science, Universiti Malaya, Kuala Lumpur, Malaysia., Ibrahim MF; Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Malaysia., Show PL; Zhejiang Provincial Key Laboratory for Subtropical Water Environment and Marine Biological Resources Protection, Wenzhou University, Wenzhou, China.; Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Semenyih, Malaysia.; Department of Sustainable Engineering, Saveetha School of Engineering, SIMATS, Chennai, India.; Department of Chemical Engineering, Khalifa University, Abu Dhabi, UAE., Wan-Mohtar WAAQI; Functional Omics and Bioprocess Development Laboratory, Institute of Biological Sciences, Faculty of Science, Universiti Malaya, Kuala Lumpur, Malaysia.; Solar Research Institute (SRI), School of Electrical Engineering, College of Engineering, Universiti Teknologi MARA (UiTM), Shah Alam, Malaysia.
المصدر: Bioengineered [Bioengineered] 2023 Dec; Vol. 14 (1), pp. 2262203. Date of Electronic Publication: 2023 Oct 04.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101581063 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-5987 (Electronic) Linking ISSN: 21655979 NLM ISO Abbreviation: Bioengineered Subsets: MEDLINE
أسماء مطبوعة: Publication: 2015- : Philadelphia, PA : Taylor & Francis
Original Publication: Austin : Landes Bioscience
مواضيع طبية MeSH: Hibiscus*, Biomass ; Cell Culture Techniques ; Sucrose ; Acetates
مستخلص: The versatility of a well-known fibrous crop, Hibiscus cannabinus (kenaf) is still relatively new to many. Kenaf's potential applications, which can be extended even into critical industries such as pharmaceutical and food industries, have always been overshadowed by its traditionally grown fiber. Therefore, this study aimed to venture into the biotechnological approach in reaping the benefits of kenaf through plant cell suspension culture to maximize the production of kenaf callus biomass (KCB) and exopolysaccharide (EPS), which is deemed to be more sustainable. A growth curve was established which indicates that cultivating kenaf callus in suspension culture for 22 days gives the highest KCB (9.09 ± 1.2 g/L) and EPS (1.1 ± 0.02 g/L). Using response surface methodology (RSM), it was found that sucrose concentration, agitation speed, and naphthalene acetic acid (NAA) concentration can affect the production of KCB and EPS significantly ( p  < 0.05) while 2,4-dichlorophenoxy acetic acid (2,4-D) was deemed insignificant. To maximize the final yield of KCB and EPS, the final optimized variables are 50 g/L sucrose, 147.02 rpm, and 2 mg/L of NAA. To conclude, the optimized parameters for the cell suspension culture of kenaf callus serve as the blueprint for any sustainable large-scale production in the future and provide an alternative cultivating method to kenaf traditional farming.
References: Food Chem. 2022 Jan 30;368:130772. (PMID: 34399182)
Pharmaceutics. 2022 May 20;14(5):. (PMID: 35631686)
Carbohydr Polym. 2015 Jan 22;115:152-9. (PMID: 25439880)
AIMS Microbiol. 2020 Oct 22;6(4):379-400. (PMID: 33364534)
Asian Pac J Trop Biomed. 2014 May;4(Suppl 1):S510-5. (PMID: 25183141)
Planta. 2000 Jan;210(2):261-8. (PMID: 10664132)
Life (Basel). 2020 Sep 28;10(10):. (PMID: 32998223)
Prikl Biokhim Mikrobiol. 2004 Jan-Feb;40(1):94-7. (PMID: 15029707)
Nat Protoc. 2011 Jun;6(6):715-42. (PMID: 21637194)
Front Bioeng Biotechnol. 2023 Apr 05;11:1181822. (PMID: 37091340)
Sci Rep. 2021 Nov 29;11(1):23079. (PMID: 34845290)
Food Chem. 2021 May 15;344:128582. (PMID: 33199120)
Plants (Basel). 2021 Sep 14;10(9):. (PMID: 34579432)
Int J Biol Macromol. 2018 Jun;112:101-109. (PMID: 29374531)
Plant Biotechnol (Tokyo). 2020 Mar 25;37(1):9-14. (PMID: 32362743)
Phytochemistry. 2008 Feb;69(4):873-81. (PMID: 18037144)
J Agric Food Chem. 2001 Aug;49(8):3768-71. (PMID: 11513663)
Food Chem X. 2021 Nov 20;12:100168. (PMID: 34877528)
Evid Based Complement Alternat Med. 2013;2013:549705. (PMID: 23606884)
فهرسة مساهمة: Keywords: Hibiscus cannabinus; Life on land; bioreactor; exopolysaccharide; kenaf; optimization
Local Abstract: [plain-language-summary] The optimized cell cultivation for plant kenaf callus is 22 daysSucrose, agitation and NAA concentration stimulates the production of KCB and EPSHighest KCB and EPS was generated at 13.41 g/L and 1.86 g/L, respectivelyMaximum production blueprint for KCB and EPS require 50 g/L sucrose, 2 mg/L of NAA and 147.02 rpm.
المشرفين على المادة: 57-50-1 (Sucrose)
0 (Acetates)
تواريخ الأحداث: Date Created: 20231004 Date Completed: 20231005 Latest Revision: 20231007
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10552614
DOI: 10.1080/21655979.2023.2262203
PMID: 37791464
قاعدة البيانات: MEDLINE
الوصف
تدمد:2165-5987
DOI:10.1080/21655979.2023.2262203