دورية أكاديمية
In silico prospection of Lactobacillus acidophilus strains with potential probiotic activity.
| العنوان: | In silico prospection of Lactobacillus acidophilus strains with potential probiotic activity. |
|---|---|
| المؤلفون: | Dias RS; Post-Graduate Program in Health Sciences, Universidade Federal do Rio Grande - FURG, Faculty of Medicine, Academic Area of the University Hospital, Rio Grande, RS, Brazil. rafaellasinnott@gmail.com., Kremer FS; Bioinformatics Laboratory, Technological Development Center, Federal University of Pelotas, Capão do Leão, Rio Grande do Sul, Brazil., da Costa de Avila LF; Post-Graduate Program in Health Sciences, Universidade Federal do Rio Grande - FURG, Faculty of Medicine, Academic Area of the University Hospital, Rio Grande, RS, Brazil. |
| المصدر: | Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2023 Dec; Vol. 54 (4), pp. 2733-2743. Date of Electronic Publication: 2023 Oct 06. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer International Publishing Country of Publication: Brazil NLM ID: 101095924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1678-4405 (Electronic) Linking ISSN: 15178382 NLM ISO Abbreviation: Braz J Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2019- : Switzerland, AG : Springer International Publishing Original Publication: Rio de Janeiro, RJ, Brasil : Sociedade Brasileira de Microbiologia |
| مواضيع طبية MeSH: | Lactobacillus acidophilus*/genetics , Probiotics*/metabolism, Lactobacillus/metabolism ; Lactic Acid/metabolism ; Acetic Acid/metabolism |
| مستخلص: | Lactic acid bacteria (LAB) are fermentative microorganisms and perform different roles in biotechnological processes, mainly in the food and pharmaceutical industries. Among the LAB, Lactobacillus acidophilus is a species that deserves to be highlighted for being used both in prophylaxis and in the treatment of pathologies. Most of the metabolites produced by this species are linked to the inhibition of pathogens. In this study, we utilized a pangenomic and metabolic annotation analysis using Roary and BlastKOALA, ML-based probiotic activity prediction with iProbiotic and whole-genome similarity using ANI to identify strains of L. acidophilus with potential probiotic activity. According to the results in BlastKOALA and iProbiotics, L. acidophilus NCTC 13721 had the greatest potential among the 64 strains tested, both in terms of its ability to be a Lactobacillus spp. probiotic, when in the amount of genes involved in the metabolism of organic acids and quorum sensing. In addition, DSM 20079 proved to be promising for prospecting new probiotic Lactobacillus from BlastKOALA analyses, as they presented similar results in the number of genes involved in the production of lactic acid, acetic acid, hydrogen peroxide, except for quorum sensing where the NCTC 13721 strain had 14 more genes. L. acidophilus NCTC 13721 and L. acidophilus La-5 strains showed greater ability to be Lactobacillus spp. probiotic capacity, showing 84.8% and 51.9% capacity in the iProbiotics tool, respectively. When analyzed in ANI, none of the evaluated strains showed genomic similarity with NCTC 13721. In contrast, the DSM 20079 strain showed genomic similarity with all evaluated strains except NCTC 13721. Furthermore, eight strains with characteristics with approximately 100% genomic similarity to La-5 were listed: S20_1, LA-5, FSI4, APC2845, LA-G80-111, DS1_1A, LA1, and BCRC 14065. Therefore, according to the findings in iProbiotics and BlastKoala, among the 64 strains evaluated, NCTC 13721 is the most promising strain to be used for future in vitro studies. (© 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.) |
| References: | Caries Res. 2017;51(2):87-95. (PMID: 28118640) Microbiologyopen. 2021 Feb;10(2):e1173. (PMID: 33970542) Mol Biol Evol. 2020 Mar 1;37(3):933-939. (PMID: 31848603) Genome Announc. 2013 Nov 27;1(6):. (PMID: 24285665) Anaerobe. 2020 Apr;62:102103. (PMID: 31525452) J Gen Appl Microbiol. 2011;57(4):197-206. (PMID: 21914968) J Appl Microbiol. 2022 Jan;132(1):592-604. (PMID: 34216519) Clin Infect Dis. 2015 May 15;60 Suppl 2:S98-107. (PMID: 25922408) Microorganisms. 2020 Sep 21;8(9):. (PMID: 32967209) Microbiol Rev. 1995 Mar;59(1):143-69. (PMID: 7535888) J Microbiol Biotechnol. 2022 Oct 28;32(10):1226-1233. (PMID: 36196014) BMJ Open. 2015 Jan 14;5(1):e006474. (PMID: 25588782) FEMS Microbiol Lett. 2013 Dec;349(2):77-87. (PMID: 24152174) Genome Announc. 2013 Jun 20;1(3):. (PMID: 23788546) Nat Commun. 2018 Nov 30;9(1):5114. (PMID: 30504855) Int J Syst Evol Microbiol. 2020 Apr;70(4):2782-2858. (PMID: 32293557) Res Microbiol. 2017 Nov - Dec;168(9-10):782-792. (PMID: 28435139) Int J Syst Evol Microbiol. 2018 Jul;68(7):2386-2392. (PMID: 29792589) Molecules. 2019 Jun 26;24(13):. (PMID: 31247973) Crit Rev Food Sci Nutr. 1999 Jan;39(1):13-126. (PMID: 10028126) Int J Antimicrob Agents. 2022 Jan;59(1):106471. (PMID: 34757134) Nat Rev Microbiol. 2011 Jan;9(1):27-38. (PMID: 21113182) Cell Rep Med. 2021 Sep 07;2(9):100393. (PMID: 34622230) Proc Natl Acad Sci U S A. 2005 Mar 15;102(11):3906-12. (PMID: 15671160) Brief Bioinform. 2022 Jan 17;23(1):. (PMID: 34849572) Genome Announc. 2015 Jan 15;3(1):. (PMID: 25593259) PLoS Negl Trop Dis. 2021 Feb 18;15(2):e0009104. (PMID: 33600419) Front Cell Infect Microbiol. 2020 Sep 25;10:538077. (PMID: 33102249) Microorganisms. 2020 Mar 17;8(3):. (PMID: 32192182) Genome Announc. 2015 Apr 09;3(2):. (PMID: 25858829) Am J Physiol Gastrointest Liver Physiol. 2009 Sep;297(3):G506-13. (PMID: 19589943) Nutrients. 2020 Apr 16;12(4):. (PMID: 32316181) Biocontrol Sci. 2019;24(4):185-192. (PMID: 31875610) J Mol Biol. 2016 Feb 22;428(4):726-731. (PMID: 26585406) Clin Nutr. 2017 Feb;36(1):85-92. (PMID: 26732026) Genome Announc. 2018 Jun 28;6(26):. (PMID: 29954914) RSC Adv. 2020 Apr 29;10(28):16737-16748. (PMID: 35498845) Anaerobe. 2019 Feb;55:142-151. (PMID: 30576791) Microb Genom. 2021 Jun;7(6):. (PMID: 34100697) Microorganisms. 2023 Mar 20;11(3):. (PMID: 36985366) Foods. 2022 Dec 24;12(1):. (PMID: 36613306) J Infect Dev Ctries. 2016 Mar 31;10(3):214-21. (PMID: 27031452) Nat Commun. 2015 Sep 29;6:8322. (PMID: 26415554) Microorganisms. 2021 Sep 20;9(9):. (PMID: 34576887) Probiotics Antimicrob Proteins. 2012 Dec;4(4):217-26. (PMID: 26782181) Front Pharmacol. 2018 Feb 08;9:83. (PMID: 29472862) Open Access Maced J Med Sci. 2019 Apr 10;7(7):1053-1058. (PMID: 31049080) |
| فهرسة مساهمة: | Keywords: Bioinformatics; Lactic acid; Lactic acid bacteria; Lactobacillus; Pangenome; Probiotics |
| المشرفين على المادة: | 33X04XA5AT (Lactic Acid) Q40Q9N063P (Acetic Acid) |
| تواريخ الأحداث: | Date Created: 20231006 Date Completed: 20231204 Latest Revision: 20231205 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC10689588 |
| DOI: | 10.1007/s42770-023-01139-3 |
| PMID: | 37801223 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1678-4405 |
|---|---|
| DOI: | 10.1007/s42770-023-01139-3 |