دورية أكاديمية
أعرض في EDS

The Effect of Calcium hydroxide, Glass Ionomer and light cured resin modified calcium silicate on viability, proliferation and differentiation of stem cells from human exfoliated deciduous teeth.

التفاصيل البيبلوغرافية
العنوان: The Effect of Calcium hydroxide, Glass Ionomer and light cured resin modified calcium silicate on viability, proliferation and differentiation of stem cells from human exfoliated deciduous teeth.
المؤلفون: Shalaby RA; Department of Pediatric Dentistry and Public Health, Zagazig University, Zagazig, Egypt. rashalaby@zu.edu.eg., Abdel-Aziz AM; Department of Pediatric Dentistry and Public Health, Ain Shams University, Cairo, Egypt., Rashed LA; Department of Medical Biochemistry and Molecular Biology, Cairo University, Cairo, Egypt., Radwan MZ; Department of Pediatric Dentistry and Public Health, Ain Shams University, Cairo, Egypt.
المصدر: BMC oral health [BMC Oral Health] 2023 Oct 06; Vol. 23 (1), pp. 721. Date of Electronic Publication: 2023 Oct 06.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101088684 Publication Model: Electronic Cited Medium: Internet ISSN: 1472-6831 (Electronic) Linking ISSN: 14726831 NLM ISO Abbreviation: BMC Oral Health Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2001-
مواضيع طبية MeSH: Calcium Hydroxide*/pharmacology , Calcium Hydroxide*/therapeutic use , Calcium Compounds*/pharmacology, Humans ; Silicates/pharmacology ; Cell Differentiation ; Stem Cells ; Tooth, Deciduous ; Cell Proliferation ; Dental Pulp
مستخلص: Background: Vital pulp therapy, based on the use of stem cells, has promising research and therapeutic applications in dentistry. It is essential to understand the direct effect of capping materials on the dental pulp stem cells of primary teeth, which contribute to the healing powers of the tooth. The aim of this study is to evaluate the effect of different capping materials (Calcium Hydroxide (DyCal®) - Glass Ionomer (Fuji IX®) and light-cured resin modified calcium silicate (TheraCal LC®)) on the viability, proliferation, and differentiation of stem cells from human exfoliated deciduous teeth (SHEDs).
Methods: SHEDs were isolated from extracted primary teeth, then divided into four groups and each of the capping materials were applied to the stem cells as follows: group I the controls, group II with Ca(OH)2, group III with the GIC, and group IV with the Theracal LC. For all groups assessment of viability and proliferation rate was done using the MTT cell proliferation assay. Also, Differentiation was evaluated by measuring the gene expression of Alkaline phosphatase enzyme activity (ALP) and Dentin matrix protein-1 (DMP1) through quantitative real-time PCR. Morphological assessment was conducted using Alizarin Red S staining. All evaluations were performed after 7 and 14 days of culture.
Results: TheraCal LC showed the highest values of proliferation, which was significant only compared to the control group after 2 weeks (p = 0.012). After one week, TheraCal LC showed the highest significant values of ALP and DMP1 compared to all other groups (p < 0.001).
Conclusion: The three materials under study are biocompatible, maintain viability, and stimulate the proliferation and differentiation of SHEDs. However, TheraCal LC allows better proliferation of SHEDs than Dycal Ca(OH)2 and Fuji IX GIC.
(© 2023. BioMed Central Ltd., part of Springer Nature.)
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فهرسة مساهمة: Keywords: Calcium hydroxide; Differentiation; Glass ionomer cement; Primary teeth; Proliferation; SHEDs; TheraCal LC; Vital pulp therapy
المشرفين على المادة: 54479-90-2 (Dycal)
PF5DZW74VN (Calcium Hydroxide)
0 (TheraCal)
S4255P4G5M (calcium silicate)
0 (glass ionomer)
0 (Calcium Compounds)
0 (Silicates)
تواريخ الأحداث: Date Created: 20231006 Date Completed: 20231102 Latest Revision: 20231118
رمز التحديث: 20231118
مُعرف محوري في PubMed: PMC10557230
DOI: 10.1186/s12903-023-03429-6
PMID: 37803363
قاعدة البيانات: MEDLINE
الوصف
تدمد:1472-6831
DOI:10.1186/s12903-023-03429-6