دورية أكاديمية
Exploring the α-amylase-inhibitory properties of tannin-rich extracts of Cytinus hypocistis on starch digestion.
| العنوان: | Exploring the α-amylase-inhibitory properties of tannin-rich extracts of Cytinus hypocistis on starch digestion. |
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| المؤلفون: | Correa VG; Departamento de Bioquímica, Universidade Estadual de Maringá, Maringá 87020-900, Brazil., Garcia-Manieri JAA; Departamento de Bioquímica, Universidade Estadual de Maringá, Maringá 87020-900, Brazil., Silva AR; Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal; Laboratório Associado para a Sustentabilidade e Tecnologia em Regiões de Montanha (SusTEC), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal; Departamento de Ciencias Farmacéuticas. Facultad de Farmacia, CIETUS-IBSAL, Universidad de Salamanca, 37007 Salamanca, España., Backes E; Departamento de Bioquímica, Universidade Estadual de Maringá, Maringá 87020-900, Brazil., Corrêa RCG; Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal; Programa de Pós-Graduação em Tecnologias Limpas, Instituto Cesumar de Ciência, Tecnologia e Inovação-ICETI, Universidade Cesumar-UNICESUMAR, Maringá 87050-900, Brazil., Barros L; Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal; Laboratório Associado para a Sustentabilidade e Tecnologia em Regiões de Montanha (SusTEC), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal., Bracht A; Departamento de Bioquímica, Universidade Estadual de Maringá, Maringá 87020-900, Brazil., Peralta RM; Departamento de Bioquímica, Universidade Estadual de Maringá, Maringá 87020-900, Brazil. Electronic address: rmperalta@uem.br. |
| المصدر: | Food research international (Ottawa, Ont.) [Food Res Int] 2023 Nov; Vol. 173 (Pt 1), pp. 113260. Date of Electronic Publication: 2023 Jul 10. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science Country of Publication: Canada NLM ID: 9210143 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7145 (Electronic) Linking ISSN: 09639969 NLM ISO Abbreviation: Food Res Int Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Ottawa, Ontario, Canada : Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science, c1992- |
| مواضيع طبية MeSH: | Tannins*/pharmacology , Diabetes Mellitus, Experimental*, Mice ; Animals ; Starch ; Plant Extracts/pharmacology ; alpha-Amylases/pharmacology ; Hydrolyzable Tannins ; Glucose ; Digestion |
| مستخلص: | Cytinus hypocistis(L.) L. is an edible parasitic plant that grows within the roots of its host. In addition to its use as famine food in the past, it is also tradidionally used for treating several illnesses such as intestinal problems, inflammations, tumors, and bleeding. This species is rich in hydrolysable tannins, compounds often associated with inhibiting starch digestion. Therefore, the present work investigated how effectively C. hypocistis tannin-rich extracts inhibited enzymes involved in starch digestion and if such effect also occurs in vivo. The latter premise was approached using the starch tolerance test in mice. Two optimized hydroethanolic extracts were used, a heat-assisted and an ultrasound-assisted extract, with known hydrolysable tannin content. Both extracts demonstrated potent inhibition of α-amylase. Inhibitions were of the mixed type with inhibitor constants in the 15 μg/mL range. The inhibition of the intestinal α-glucosidase was at least ten times less effective. The inhibition of the α-amylase was negatively affected by in vitro gastrointestinal digestion and bovine serum albumin. In vivo, both extracts inhibited starch digestion at doses between 100 and 400 mg/mL in healthy mice. The highest doses of the ultrasound and heat extracts diminished the peak glucose levels in the starch tolerance test by 46 and 59.3%, respectively. In streptozotocin diabetic mice, this inhibition occurred only at the dose of 400 mg/mL. Under this condition, diminution of the peak glucose concentration in the starch tolerance test was equal to 36.7% and 48.8% for the ultrasound and heat extracts, respectively. Maltose digestion was not inhibited by the C. hypocistis extracts. Qualitatively and quantitatively, thus, the actions of both extracts were similar. The results allow adding a new biological property to C. hypocistis, namely, the ability to decrease the hyper-glycemic excursion after a starch-rich meal, propitiating at the same time a diminished caloric intake. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Diabetes; Gastrointestinal digestion; Hydrolysable tannins; α-amylase inhibition |
| المشرفين على المادة: | 0 (Tannins) 9005-25-8 (Starch) 0 (Plant Extracts) EC 3.2.1.1 (alpha-Amylases) 0 (Hydrolyzable Tannins) IY9XDZ35W2 (Glucose) |
| تواريخ الأحداث: | Date Created: 20231007 Date Completed: 20231009 Latest Revision: 20231010 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.foodres.2023.113260 |
| PMID: | 37803573 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-7145 |
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| DOI: | 10.1016/j.foodres.2023.113260 |