دورية أكاديمية
أعرض في EDS

The LIKE SEX FOUR 1-malate dehydrogenase complex functions as a scaffold to recruit β-amylase to promote starch degradation.

التفاصيل البيبلوغرافية
العنوان: The LIKE SEX FOUR 1-malate dehydrogenase complex functions as a scaffold to recruit β-amylase to promote starch degradation.
المؤلفون: Liu J; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China., Wang X; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China., Guan Z; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China., Wu M; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China., Wang X; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China., Fan R; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China., Zhang F; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China., Yan J; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China., Liu Y; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China., Zhang D; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China., Yin P; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China., Yan J; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China.
المصدر: The Plant cell [Plant Cell] 2023 Dec 21; Vol. 36 (1), pp. 194-212.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 9208688 Publication Model: Print Cited Medium: Internet ISSN: 1532-298X (Electronic) Linking ISSN: 10404651 NLM ISO Abbreviation: Plant Cell Subsets: MEDLINE
أسماء مطبوعة: Publication: 2021- : [Oxford] : Oxford University Press
Original Publication: Rockville, MD : American Society of Plant Physiologists, c1989-
مواضيع طبية MeSH: Arabidopsis Proteins*/metabolism , Arabidopsis*/metabolism , beta-Amylase*/metabolism, Malate Dehydrogenase/metabolism ; Molecular Docking Simulation ; Cryoelectron Microscopy ; Starch/metabolism ; Glucans/metabolism ; Phosphoric Monoester Hydrolases/metabolism ; Protein Serine-Threonine Kinases/metabolism
مستخلص: In plant leaves, starch is composed of glucan polymers that accumulate in chloroplasts as the products of photosynthesis during the day; starch is mobilized at night to continuously provide sugars to sustain plant growth and development. Efficient starch degradation requires the involvement of several enzymes, including β-amylase and glucan phosphatase. However, how these enzymes cooperate remains largely unclear. Here, we show that the glucan phosphatase LIKE SEX FOUR 1 (LSF1) interacts with plastid NAD-dependent malate dehydrogenase (MDH) to recruit β-amylase (BAM1), thus reconstituting the BAM1-LSF1-MDH complex. The starch hydrolysis activity of BAM1 drastically increased in the presence of LSF1-MDH in vitro. We determined the structure of the BAM1-LSF1-MDH complex by a combination of cryo-electron microscopy, crosslinking mass spectrometry, and molecular docking. The starch-binding domain of the dual-specificity phosphatase and carbohydrate-binding module of LSF1 was docked in proximity to BAM1, thus facilitating BAM1 access to and hydrolysis of the polyglucans of starch, thus revealing the molecular mechanism by which the LSF1-MDH complex improves the starch degradation activity of BAM1. Moreover, LSF1 is phosphatase inactive, and the enzymatic activity of MDH was dispensable for starch degradation, suggesting nonenzymatic scaffold functions for LSF1-MDH in starch degradation. These findings provide important insights into the precise regulation of starch degradation.
Competing Interests: Conflict of interest statement. None declared.
(© American Society of Plant Biologists 2023. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
References: Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):. (PMID: 33531363)
Nucleic Acids Res. 2022 Jan 7;50(D1):D1522-D1527. (PMID: 34871441)
Plant Cell. 2008 Apr;20(4):1040-58. (PMID: 18390594)
Plant Physiol. 2006 Jul;141(3):840-50. (PMID: 16698902)
Ultramicroscopy. 2013 Dec;135:24-35. (PMID: 23872039)
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21. (PMID: 20124702)
Nat Methods. 2012 Sep;9(9):904-6. (PMID: 22772728)
Plant Cell. 2011 Nov;23(11):4096-111. (PMID: 22100529)
PLoS Biol. 2015 Feb 24;13(2):e1002080. (PMID: 25710501)
Elife. 2016 Nov 15;5:. (PMID: 27845625)
Plant Physiol. 2022 Jan 20;188(1):191-207. (PMID: 34662400)
Plant Physiol. 2010 Feb;152(2):711-22. (PMID: 20018599)
Trends Plant Sci. 2022 May;27(5):488-501. (PMID: 34848143)
J Struct Biol. 2022 Sep;214(3):107885. (PMID: 35961473)
Protein Sci. 2020 Aug;29(8):1851-1857. (PMID: 32557917)
Annu Rev Plant Biol. 2020 Apr 29;71:217-245. (PMID: 32075407)
Proc Natl Acad Sci U S A. 2010 Aug 31;107(35):15379-84. (PMID: 20679247)
Cell Res. 2018 Apr;28(4):448-461. (PMID: 29540758)
J Struct Biol. 2016 Jan;193(1):1-12. (PMID: 26592709)
Nat Methods. 2017 Mar;14(3):290-296. (PMID: 28165473)
Plant Physiol. 2014 Mar;164(3):1175-90. (PMID: 24453164)
Arabidopsis Book. 2012;10:e0160. (PMID: 23393426)
Nat Methods. 2022 Jun;19(6):679-682. (PMID: 35637307)
Plant Sci. 2018 Nov;276:163-170. (PMID: 30348315)
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W375-83. (PMID: 17452350)
Curr Opin Struct Biol. 2016 Oct;40:62-69. (PMID: 27498086)
Plant Cell. 2019 Sep;31(9):2169-2186. (PMID: 31266901)
Proc Natl Acad Sci U S A. 2014 May 20;111(20):7272-7. (PMID: 24799671)
Plant Cell. 2013 Jun;25(6):2302-14. (PMID: 23832589)
FEBS Lett. 2006 Sep 4;580(20):4872-6. (PMID: 16914145)
Plant Physiol. 2010 Feb;152(2):685-97. (PMID: 20018601)
Plant Mol Biol. 2022 Mar;108(4-5):343-361. (PMID: 34387795)
New Phytol. 2017 May;214(3):943-951. (PMID: 28277621)
Nature. 2021 Aug;596(7873):583-589. (PMID: 34265844)
Trends Plant Sci. 2014 Jul;19(7):471-8. (PMID: 24534096)
Allergy. 2019 May;74(5):1009-1013. (PMID: 30515829)
Plant Cell. 2009 Jan;21(1):334-46. (PMID: 19141707)
Plant Cell. 2001 Aug;13(8):1907-18. (PMID: 11487701)
Plant Cell. 2016 Aug;28(8):1860-78. (PMID: 27436713)
Nat Protoc. 2018 Mar;13(3):478-494. (PMID: 29419816)
Nat Commun. 2019 Jul 30;10(1):3404. (PMID: 31363125)
J Struct Biol. 2005 Oct;152(1):36-51. (PMID: 16182563)
Plant Physiol. 2005 Jan;137(1):242-52. (PMID: 15618411)
Plant Cell. 2017 Jul;29(7):1657-1677. (PMID: 28684429)
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32. (PMID: 15572765)
Mol Cell. 2015 Jan 22;57(2):261-72. (PMID: 25544560)
Plant Physiol. 2017 Dec;175(4):1525-1535. (PMID: 29066669)
Mol Plant. 2014 Jan;7(1):170-86. (PMID: 24198233)
Int J Biol Macromol. 2018 Jul 1;113:329-337. (PMID: 29481953)
Proc Natl Acad Sci U S A. 2002 May 14;99(10):7166-71. (PMID: 12011472)
Plant J. 2005 Feb;41(4):595-605. (PMID: 15686522)
Plant Physiol. 2022 Mar 28;188(4):1979-1992. (PMID: 34958379)
Plant Physiol. 2014 Dec;166(4):1748-63. (PMID: 25293962)
Nat Methods. 2017 Apr;14(4):331-332. (PMID: 28250466)
Biochemistry. 1999 Jun 1;38(22):7050-61. (PMID: 10353816)
Cell Metab. 2013 May 7;17(5):756-67. (PMID: 23663739)
Mol Biosyst. 2011 Mar;7(3):718-30. (PMID: 21085740)
Biochemistry. 2005 Apr 5;44(13):5106-16. (PMID: 15794648)
Plant Cell. 2020 Jul;32(7):2325-2344. (PMID: 32354788)
Nat Protoc. 2010 May;5(5):883-97. (PMID: 20431534)
Annu Rev Plant Biol. 2005;56:73-98. (PMID: 15862090)
Plant Cell. 2018 Aug;30(8):1745-1769. (PMID: 29934433)
Nat Rev Neurol. 2018 Oct;14(10):606-617. (PMID: 30143794)
Curr Biol. 2016 Feb 8;26(3):362-70. (PMID: 26774787)
Plant Direct. 2020 Feb 11;4(2):e00199. (PMID: 32072133)
المشرفين على المادة: 0 (Arabidopsis Proteins)
EC 1.1.1.37 (Malate Dehydrogenase)
EC 3.2.1.2 (beta-Amylase)
9005-25-8 (Starch)
0 (Glucans)
EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
EC 2.7.11.1 (BAM1 protein, Arabidopsis)
EC 2.7.11.1 (Protein Serine-Threonine Kinases)
تواريخ الأحداث: Date Created: 20231007 Date Completed: 20231222 Latest Revision: 20231223
رمز التحديث: 20231223
مُعرف محوري في PubMed: PMC10734626
DOI: 10.1093/plcell/koad259
PMID: 37804098
قاعدة البيانات: MEDLINE
الوصف
تدمد:1532-298X
DOI:10.1093/plcell/koad259