دورية أكاديمية
Development and evaluation of a real-time quantitative PCR for the detection of equine infectious anemia virus.
| العنوان: | Development and evaluation of a real-time quantitative PCR for the detection of equine infectious anemia virus. |
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| المؤلفون: | Li S; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agriculture Sciences , Harbin, China., Guo K; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agriculture Sciences , Harbin, China., Wang X; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agriculture Sciences , Harbin, China., Lin Y; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agriculture Sciences , Harbin, China., Wang J; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agriculture Sciences , Harbin, China., Wang Y; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agriculture Sciences , Harbin, China., Du C; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agriculture Sciences , Harbin, China., Hu Z; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agriculture Sciences , Harbin, China.; WOAH Reference Laboratory for Equine Infectious Anemia, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences , Harbin, China., Wang X; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agriculture Sciences , Harbin, China.; WOAH Reference Laboratory for Equine Infectious Anemia, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences , Harbin, China. |
| المصدر: | Microbiology spectrum [Microbiol Spectr] 2023 Dec 12; Vol. 11 (6), pp. e0259923. Date of Electronic Publication: 2023 Oct 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: ASM Press Country of Publication: United States NLM ID: 101634614 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-0497 (Electronic) Linking ISSN: 21650497 NLM ISO Abbreviation: Microbiol Spectr Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : ASM Press, 2013- |
| مواضيع طبية MeSH: | Infectious Anemia Virus, Equine*/genetics , Equine Infectious Anemia*/diagnosis, Animals ; Horses ; Real-Time Polymerase Chain Reaction ; DNA Primers/genetics |
| مستخلص: | Importance: Equine infectious anemia (EIA) has a worldwide distribution and causes significant losses to the equine industry worldwide. A reliable detection method is necessary to control the transmission of EIA virus (EIAV). Currently, most of the available real-time PCR assays, including the qPCR of recommended by WOAH, are developed according to the sequences of European or American EIAV strains; however, the primers and probe sequences have low homology with Asian EIAV strains. To the best of our knowledge, no qPCR method capable of the well detection of Asian EIAV strains, especially Chinese EIAV strains, has been published to date. The development of a sensitive, specific, and rapid qPCR assay for the detection of the EIAV strains is therefore of great importance. Competing Interests: The authors declare no conflicts of interest. |
| معلومات مُعتمدة: | 2021YFD1800500 MOST | National Key Research and Development Program of China (NKPs); 2022YFD1800200 MOST | National Key Research and Development Program of China (NKPs) |
| فهرسة مساهمة: | Keywords: equine infectious anemia; gag gene; real-time quantitative PCR; tat gene |
| المشرفين على المادة: | 0 (DNA Primers) |
| تواريخ الأحداث: | Date Created: 20231009 Date Completed: 20231216 Latest Revision: 20231216 |
| رمز التحديث: | 20231217 |
| مُعرف محوري في PubMed: | PMC10715080 |
| DOI: | 10.1128/spectrum.02599-23 |
| PMID: | 37811976 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2165-0497 |
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| DOI: | 10.1128/spectrum.02599-23 |