دورية أكاديمية
Sleep deprivation soon after recovery from synthetic torpor enhances tau protein dephosphorylation in the rat brain.
العنوان: | Sleep deprivation soon after recovery from synthetic torpor enhances tau protein dephosphorylation in the rat brain. |
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المؤلفون: | Hitrec T; Department of Biomedical and NeuroMotor Sciences, University of Bologna, Piazza di Porta San Donato, 2, 40126, Bologna, Italy., Squarcio F; Department of Biomedical and NeuroMotor Sciences, University of Bologna, Piazza di Porta San Donato, 2, 40126, Bologna, Italy.; Department of Psychiatry, University of Wisconsin-Madison, Madison, WI, USA., Piscitiello E; Department of Biomedical and NeuroMotor Sciences, University of Bologna, Piazza di Porta San Donato, 2, 40126, Bologna, Italy.; Centre for Applied Biomedical Research - CRBA, University of Bologna, St. Orsola Hospital, Bologna, Italy., Cerri M; Department of Biomedical and NeuroMotor Sciences, University of Bologna, Piazza di Porta San Donato, 2, 40126, Bologna, Italy., Martelli D; Department of Biomedical and NeuroMotor Sciences, University of Bologna, Piazza di Porta San Donato, 2, 40126, Bologna, Italy.; Centre for Applied Biomedical Research - CRBA, University of Bologna, St. Orsola Hospital, Bologna, Italy., Occhinegro A; Department of Biomedical and NeuroMotor Sciences, University of Bologna, Piazza di Porta San Donato, 2, 40126, Bologna, Italy.; Centre for Applied Biomedical Research - CRBA, University of Bologna, St. Orsola Hospital, Bologna, Italy., Taddei L; Department of Biomedical and NeuroMotor Sciences, University of Bologna, Piazza di Porta San Donato, 2, 40126, Bologna, Italy.; Centre for Applied Biomedical Research - CRBA, University of Bologna, St. Orsola Hospital, Bologna, Italy., Tupone D; Department of Biomedical and NeuroMotor Sciences, University of Bologna, Piazza di Porta San Donato, 2, 40126, Bologna, Italy.; Department of Neurological Surgery, Oregon Health and Science University, Portland, OR, USA., Amici R; Department of Biomedical and NeuroMotor Sciences, University of Bologna, Piazza di Porta San Donato, 2, 40126, Bologna, Italy., Luppi M; Department of Biomedical and NeuroMotor Sciences, University of Bologna, Piazza di Porta San Donato, 2, 40126, Bologna, Italy. marco.luppi@unibo.it.; Centre for Applied Biomedical Research - CRBA, University of Bologna, St. Orsola Hospital, Bologna, Italy. marco.luppi@unibo.it. |
المصدر: | Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology [J Comp Physiol B] 2024 Jun; Vol. 194 (3), pp. 347-368. Date of Electronic Publication: 2023 Oct 09. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 8413200 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-136X (Electronic) Linking ISSN: 01741578 NLM ISO Abbreviation: J Comp Physiol B Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Berlin ; New York : Springer-Verlag, [c1984- |
مواضيع طبية MeSH: | Sleep Deprivation*/metabolism , Sleep Deprivation*/physiopathology , tau Proteins*/metabolism , Torpor*/physiology , Brain*/metabolism , Rats, Wistar*, Animals ; Male ; Phosphorylation ; Rats ; Microglia/metabolism |
مستخلص: | Neuronal Tau protein hyperphosphorylation (PPtau) is a hallmark of tauopathic neurodegeneration. However, a reversible brain PPtau occurs in mammals during either natural or "synthetic" torpor (ST), a transient deep hypothermic state that can be pharmacologically induced in rats. Since in both conditions a high sleep pressure builds up during the regaining of euthermia, the aim of this work was to assess the possible role of post-ST sleep in PPtau dephosphorylation. Male rats were studied at the hypothermic nadir of ST, and 3-6 h after the recovery of euthermia, after either normal sleep (NS) or total sleep deprivation (SD). The effects of SD were studied by assessing: (i) deep brain temperature (Tb); (ii) immunofluorescent staining for AT8 (phosphorylated Tau) and Tau-1 (non-phosphorylated Tau), assessed in 19 brain structures; (iii) different phosphorylated forms of Tau and the main cellular factors involved in Tau phospho-regulation, including pro- and anti-apoptotic markers, assessed through western blot in the parietal cortex and hippocampus; (iv) systemic factors which are involved in natural torpor; (v) microglia activation state, by considering morphometric variations. Unexpectedly, the reversibility of PPtau was more efficient in SD than in NS animals, and was concomitant with a higher Tb, higher melatonin plasma levels, and a higher frequency of the microglia resting phenotype. Since the reversibility of ST-induced PPtau was previously shown to be driven by a latent physiological molecular mechanism triggered by deep hypothermia, short-term SD soon after the regaining of euthermia seems to boost the possible neuroprotective effects of this mechanism. (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
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معلومات مُعتمدة: | 2018/0370 Fondazione Cassa di Risparmio in Bologna; 4000123556 European Space Agency |
فهرسة مساهمة: | Keywords: Brain temperature; Deep hypothermia; Gentle handling; Melatonin; Microglia; Sleep pressure |
المشرفين على المادة: | 0 (tau Proteins) |
تواريخ الأحداث: | Date Created: 20231009 Date Completed: 20240709 Latest Revision: 20240802 |
رمز التحديث: | 20240802 |
DOI: | 10.1007/s00360-023-01516-2 |
PMID: | 37812305 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1432-136X |
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DOI: | 10.1007/s00360-023-01516-2 |