دورية أكاديمية
أعرض في EDS

Antiviral responses are shaped by heterogeneity in viral replication dynamics.

التفاصيل البيبلوغرافية
العنوان: Antiviral responses are shaped by heterogeneity in viral replication dynamics.
المؤلفون: Bruurs LJM; Oncode Institute, Hubrecht Institute-KNAW and University Medical Center Utrecht, Utrecht, the Netherlands., Müller M; Oncode Institute, Hubrecht Institute-KNAW and University Medical Center Utrecht, Utrecht, the Netherlands., Schipper JG; Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands., Rabouw HH; Oncode Institute, Hubrecht Institute-KNAW and University Medical Center Utrecht, Utrecht, the Netherlands.; Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands., Boersma S; Oncode Institute, Hubrecht Institute-KNAW and University Medical Center Utrecht, Utrecht, the Netherlands., van Kuppeveld FJM; Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands., Tanenbaum ME; Oncode Institute, Hubrecht Institute-KNAW and University Medical Center Utrecht, Utrecht, the Netherlands. m.tanenbaum@hubrecht.eu.; Department of Bionanoscience, Delft University of Technology, Delft, the Netherlands. m.tanenbaum@hubrecht.eu.
المصدر: Nature microbiology [Nat Microbiol] 2023 Nov; Vol. 8 (11), pp. 2115-2129. Date of Electronic Publication: 2023 Oct 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101674869 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2058-5276 (Electronic) Linking ISSN: 20585276 NLM ISO Abbreviation: Nat Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Publishing Group, [2016]-
مواضيع طبية MeSH: Virus Replication* , Virus Diseases*, Humans ; Signal Transduction ; Encephalomyocarditis virus/physiology ; Antiviral Agents
مستخلص: Antiviral signalling, which can be activated in host cells upon virus infection, restricts virus replication and communicates infection status to neighbouring cells. The antiviral response is heterogeneous, both quantitatively (efficiency of response activation) and qualitatively (transcribed antiviral gene set). To investigate the basis of this heterogeneity, we combined Virus Infection Real-time IMaging (VIRIM), a live-cell single-molecule imaging method, with real-time readouts of the dsRNA sensing pathway to analyse the response of human cells to encephalomyocarditis virus (EMCV) infection. We find that cell-to-cell heterogeneity in viral replication rates early in infection affect the efficiency of antiviral response activation, with lower replication rates leading to more antiviral response activation. Furthermore, we show that qualitatively distinct antiviral responses can be linked to the strength of the antiviral signalling pathway. Our analyses identify variation in early viral replication rates as an important parameter contributing to heterogeneity in antiviral response activation.
(© 2023. The Author(s).)
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المشرفين على المادة: 0 (Antiviral Agents)
تواريخ الأحداث: Date Created: 20231009 Date Completed: 20231108 Latest Revision: 20231122
رمز التحديث: 20231122
مُعرف محوري في PubMed: PMC10627821
DOI: 10.1038/s41564-023-01501-z
PMID: 37814072
قاعدة البيانات: MEDLINE
الوصف
تدمد:2058-5276
DOI:10.1038/s41564-023-01501-z