دورية أكاديمية
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Genetic analysis challenges the presence of Ixodes inopinatus in Central Europe: development of a multiplex PCR to distinguish I. inopinatus from I. ricinus.

التفاصيل البيبلوغرافية
العنوان: Genetic analysis challenges the presence of Ixodes inopinatus in Central Europe: development of a multiplex PCR to distinguish I. inopinatus from I. ricinus.
المؤلفون: Hrazdilova K; Department of Chemistry and Biochemistry, Mendel University, Brno, Czech Republic.; Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Plzen, Czech Republic., Danek O; Institute of Parasitology, Biology Center of Czech Academy of Sciences, Budějovice, Czech Republic.; Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources/CINeZ, Czech University of Life Sciences, Prague, Czech Republic., Hrbatova A; CEITEC, University of Veterinary Sciences, Brno, Czech Republic., Cervena B; CEITEC, University of Veterinary Sciences, Brno, Czech Republic.; Institute of Vertebrate Biology of the Czech Academy of Sciences, Brno, Czech Republic., Noskova E; CEITEC, University of Veterinary Sciences, Brno, Czech Republic.; Department of Botany and Zoology, Faculty of Science, Masaryk University, Brno, Czech Republic., Adamik P; Department of Zoology, Palacky University Olomouc, Olomouc, Czech Republic., Votypka J; Institute of Parasitology, Biology Center of Czech Academy of Sciences, Budějovice, Czech Republic.; Department of Parasitology, Charles University, Prague, Czech Republic., Mihalca AD; Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Cluj-Napoca, Romania., Noureddine M; Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Cluj-Napoca, Romania., Modry D; Institute of Parasitology, Biology Center of Czech Academy of Sciences, Budějovice, Czech Republic.; Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources/CINeZ, Czech University of Life Sciences, Prague, Czech Republic.; Department of Botany and Zoology, Faculty of Science, Masaryk University, Brno, Czech Republic., Zurek L; Department of Chemistry and Biochemistry, Mendel University, Brno, Czech Republic. lzurek01@gmail.com.; CEITEC, University of Veterinary Sciences, Brno, Czech Republic. lzurek01@gmail.com.; Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources/CINeZ, Czech University of Life Sciences, Prague, Czech Republic. lzurek01@gmail.com.
المصدر: Parasites & vectors [Parasit Vectors] 2023 Oct 09; Vol. 16 (1), pp. 354. Date of Electronic Publication: 2023 Oct 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101462774 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-3305 (Electronic) Linking ISSN: 17563305 NLM ISO Abbreviation: Parasit Vectors Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central
مواضيع طبية MeSH: Ixodes*, Animals ; Multiplex Polymerase Chain Reaction ; Phylogeny ; Europe ; DNA, Ribosomal/genetics
مستخلص: Background: Ixodes ricinus is an important vector of several pathogens, primarily in Europe. Recently, Ixodes inopinatus was described from Spain, Portugal, and North Africa and then reported from several European countries. In this study, a multiplex polymerase chain reaction (PCR) was developed to distinguish I. ricinus from I. inopinatus and used in the surveillance of I. inopinatus in Algeria (ALG) and three regions in the Czech Republic (CZ).
Methods: A multiplex PCR on TROSPA and sequencing of several mitochondrial (16S rDNA, COI) and nuclear markers (TROSPA, ITS2, calreticulin) were used to differentiate these two species and for a subsequent phylogenetic analysis.
Results: Sequencing of TROSPA, COI, and ITS2 separated these two species into two subclades, while 16S rDNA and calreticulin could not distinguish I. ricinus from I. inopinatus. Interestingly, 23 nucleotide positions in the TROSPA gene had consistently double peaks in a subset of ticks from CZ. Cloning of these PCR products led to a clear separation of I. ricinus and I. inopinatus indicating hybridization and introgression between these two tick taxa. Based on a multiplex PCR of TROSPA and analysis of sequences of TROSPA, COI, and ITS2, the majority of ticks in CZ were I. ricinus, no I. inopinatus ticks were found, and 10 specimens showed signs of hybridization. In contrast, most ticks in ALG were I. inopinatus, four ticks were I. ricinus, and no signs of hybridization and introgression were detected.
Conclusions: We developed a multiplex PCR method based on the TROSPA gene to differentiate I. ricinus and I. inopinatus. We demonstrate the lack of evidence for the presence of I. inopinatus in Central Europe and propose that previous studies be re-examined. Mitochondrial markers are not suitable for distinguishing I. inopinatus from I. ricinus. Furthermore, our data indicate that I. inopinatus and I. ricinus can hybridize, and the hybrids can survive in Europe.
(© 2023. BioMed Central Ltd., part of Springer Nature.)
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معلومات مُعتمدة: EXCELES, No. LX22NPO5103 National Institute of Virology and Bacteriology; GACR 21-11661S Czech Science Foundation
فهرسة مساهمة: Keywords: 16S rDNA; Algeria; COI; Czech Republic; ITS2; Ixodes inopinatus; Ixodes ricinus; TROSPA; Tick
المشرفين على المادة: 0 (DNA, Ribosomal)
تواريخ الأحداث: Date Created: 20231009 Date Completed: 20231011 Latest Revision: 20231123
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10561450
DOI: 10.1186/s13071-023-05971-2
PMID: 37814284
قاعدة البيانات: MEDLINE
الوصف
تدمد:1756-3305
DOI:10.1186/s13071-023-05971-2