دورية أكاديمية
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Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization.

التفاصيل البيبلوغرافية
العنوان: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization.
المؤلفون: Tanjore Ramanathan J; Translational Cancer Medicine Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland., Zárybnický T; Stem Cells and Metabolism Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland., Filppu P; Translational Cancer Medicine Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland., Monzo HJ; Translational Cancer Medicine Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland., Monni O; Applied Tumor Genomics Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland., Tervonen TA; Translational Cancer Medicine Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland.; Finnish genome editing center (FinGEEC), Helsinki Institute of Life Science (HiLIFE), University of Helsinki, Helsinki, Finland., Klefström J; Translational Cancer Medicine Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland.; Finnish Cancer Institute & FICAN South, Helsinki University Hospital (HUS), Helsinki, Finland., Kerosuo L; Neural Crest Development and Disease Unit, Department of Health and Human Services, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, 20892, USA. laura.kerosuo@nih.gov., Kuure S; Stem Cells and Metabolism Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland. satu.kuure@helsinki.fi.; GM-unit, Helsinki Institute of Life Science (HiLIFE), University of Helsinki, Helsinki, Finland. satu.kuure@helsinki.fi., Laakkonen P; Translational Cancer Medicine Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland. pirjo.laakkonen@helsinki.fi.; iCAN Flagship Program, University of Helsinki, Helsinki, Finland. pirjo.laakkonen@helsinki.fi.; Laboratory Animal Centre, Helsinki Institute of Life Science (HiLIFE), University of Helsinki, Helsinki, Finland. pirjo.laakkonen@helsinki.fi.
المصدر: Scientific reports [Sci Rep] 2023 Oct 11; Vol. 13 (1), pp. 17162. Date of Electronic Publication: 2023 Oct 11.
نوع المنشور: Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Neural Crest* , Enteric Nervous System*/metabolism, Mice ; Animals ; Neurons/physiology ; Gastrointestinal Tract ; Intestine, Small ; Immunoglobulins/genetics ; Immunoglobulins/metabolism ; Cell Movement/physiology
مستخلص: The immunoglobulin (Ig) superfamily members are involved in cell adhesion and migration, complex multistep processes that play critical roles in embryogenesis, wound healing, tissue formation, and many other processes, but their specific functions during embryonic development remain unclear. Here, we have studied the function of the immunoglobulin superfamily member 3 (IGSF3) by generating an Igsf3 knockout (KO) mouse model with CRISPR/Cas9-mediated genome engineering. By combining RNA and protein detection methodology, we show that during development, IGSF3 localizes to the neural crest and a subset of its derivatives, suggesting a role in normal embryonic and early postnatal development. Indeed, inactivation of Igsf3 impairs the ability of the vagal neural crest cells to migrate and normally innervate the intestine. The small intestine of Igsf3 KO mice shows reduced thickness of the muscularis externa and diminished number of enteric neurons. Also, misalignment of neurons and smooth muscle cells in the developing intestinal villi is detected. Taken together, our results suggest that IGSF3 functions contribute to the formation of the enteric nervous system. Given the essential role of the enteric nervous system in maintaining normal gastrointestinal function, our study adds to the pool of information required for further understanding the mechanisms of gut innervation and etiology behind bowel motility disorders.
(© 2023. Springer Nature Limited.)
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معلومات مُعتمدة: ZIA DE000748 United States ImNIH Intramural NIH HHS
المشرفين على المادة: 0 (Immunoglobulins)
تواريخ الأحداث: Date Created: 20231011 Date Completed: 20231102 Latest Revision: 20240210
رمز التحديث: 20240210
مُعرف محوري في PubMed: PMC10567708
DOI: 10.1038/s41598-023-44093-8
PMID: 37821496
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-023-44093-8