دورية أكاديمية
Unexpected Diversity of Pseudomonads Associated with Bacterial Leaf Spot of Cucurbits in the Southeastern United States.
| العنوان: | Unexpected Diversity of Pseudomonads Associated with Bacterial Leaf Spot of Cucurbits in the Southeastern United States. |
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| المؤلفون: | Fullem KR; Department of Plant Pathology, University of Florida, Gainesville, FL., Pena MM; Department of Entomology and Plant Pathology, Auburn University, Auburn, AL.; Department of Plant Pathology, University of Georgia, Tifton, GA., Potnis N; Department of Entomology and Plant Pathology, Auburn University, Auburn, AL., Goss EM; Department of Plant Pathology, University of Florida, Gainesville, FL., Minsavage GV; Department of Plant Pathology, University of Florida, Gainesville, FL., Iriarte FB; North Florida Research and Education Center, University of Florida, Quincy, FL., Holland A; Department of Entomology and Plant Pathology, Auburn University, Auburn, AL., Jones JB; Department of Plant Pathology, University of Florida, Gainesville, FL., Paret ML; Department of Plant Pathology, University of Florida, Gainesville, FL.; North Florida Research and Education Center, University of Florida, Quincy, FL. |
| المصدر: | Plant disease [Plant Dis] 2024 Mar; Vol. 108 (3), pp. 592-598. Date of Electronic Publication: 2024 Mar 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Phytopathological Society Country of Publication: United States NLM ID: 9882809 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0191-2917 (Print) Linking ISSN: 01912917 NLM ISO Abbreviation: Plant Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: St. Paul, Minn. : American Phytopathological Society, [1980]- |
| مواضيع طبية MeSH: | Pseudomonas syringae* , Plant Diseases*/microbiology, RNA, Ribosomal, 16S/genetics ; Georgia ; Nucleotides |
| مستخلص: | Bacterial leaf spot of cucurbits (BLS) is an emerging disease in the southeastern United States that is capable of causing widespread outbreaks under conducive conditions. Historically attributed solely to the bacterium Pseudomonas syringae pv. lachrymans , recent studies have identified additional P. syringae pathovars as causal agents of the disease. To further investigate the identity and diversity of P. syringae strains associated with BLS in the southeastern United States, 47 bacterial isolates were recovered from symptomatic cucurbits from Florida, Alabama, and Georgia. Strains were characterized using the LOPAT testing scheme, fluorescence, and pathogenicity to watermelon and squash seedlings. Thirty-eight fluorescent isolates underwent whole-genome sequencing and were further characterized with 16S rRNA, four gene multilocus sequence analysis (MLSA) phylogeny, and average nucleotide identity analysis. Thirty-four isolates were identified as members of the P. syringae species complex, including P. syringae sensu stricto (12), P. alliivorans (12), P. capsici (nine), and P. viridiflava (one). An additional four isolates were found to belong to the Pseudomonas genus outside of the syringae species complex, though they did not share 95% or greater average nucleotide identity to any validly published species and are believed to belong to three novel Pseudomonas species. These results reveal an unpredicted level of diversity of Pseudomonas strains associated with BLS in the region and show the benefits of whole-genome sequencing for strain identification. Identification of P. capsici , which is capable of causing disease at higher temperatures than P. syringae , as a causal agent of BLS may also affect management strategies in the future. Competing Interests: The author(s) declare no conflict of interest. |
| فهرسة مساهمة: | Keywords: pathogen diversity; prokaryotes; vegetables |
| المشرفين على المادة: | 0 (RNA, Ribosomal, 16S) 0 (Nucleotides) |
| تواريخ الأحداث: | Date Created: 20231012 Date Completed: 20240401 Latest Revision: 20240401 |
| رمز التحديث: | 20240401 |
| DOI: | 10.1094/PDIS-06-23-1081-SR |
| PMID: | 37822097 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0191-2917 |
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| DOI: | 10.1094/PDIS-06-23-1081-SR |