دورية أكاديمية
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The potential of gamma irradiation on antioxidant capacity and genomic alterations in Calendula officinalis.

التفاصيل البيبلوغرافية
العنوان: The potential of gamma irradiation on antioxidant capacity and genomic alterations in Calendula officinalis.
المؤلفون: Hosseini R; Biotechnology Department, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, Qazvin, Iran. Electronic address: r.hosseini@eng.ikiu.ac.ir., Mahdian N; Biotechnology Department, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, Qazvin, Iran., Yousefi Z; Biotechnology Department, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, Qazvin, Iran.
المصدر: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine [Appl Radiat Isot] 2023 Dec; Vol. 202, pp. 111034. Date of Electronic Publication: 2023 Oct 06.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Pergamon Press Country of Publication: England NLM ID: 9306253 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-9800 (Electronic) Linking ISSN: 09698043 NLM ISO Abbreviation: Appl Radiat Isot Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford ; New York : Pergamon Press, c1993-
مواضيع طبية MeSH: Antioxidants* , Calendula*, Random Amplified Polymorphic DNA Technique/methods ; Escherichia coli ; Plants ; Genomics ; DNA ; Flavonoids ; Anti-Bacterial Agents
مستخلص: There are lines of evidence that ionizing radiations such as gamma rays can cause different biological effects on plants. Marigold (Calendula officinalis L.) is a member of the family Asteraceae. It possesses profound amounts of active ingredients. The aim of this study was to evaluate the changes imposed upon different dose levels of gamma radiation on some features of Calendula officinalis such as antioxidant activity, total phenolic compounds and flavonoid contents, antibacterial activity and genomic alterations. Calendula officinalis seeds were exposed to different doses of Gamma radiation (0, 10, 15, 20 and 25 GY). Total phenolics, flavonoids, antioxidant activity (measured by DPPH assay) using methanolic extracts of plants and antibacterial activity measured by the disc diffusion assay showed significant differences to the control samples. The samples treated with 10 GY gamma rays showed the highest total phenol and flavonoid contents. Antioxidant activity significantly differed between Gamma rays dose levels and it was the highest at 25 GY. Four bacterial strains including E. coli, Bacillus subtilis and Pseudomonas aeroginosa were used for the antibacterial assay. Extracts from plants treated with 25 GY gamma rays showed the highest antibacterial activity against the 4 bacterial strains. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to study the genetic variation. The polymorphism information content (PIC) for RAPD primers ranged from 3% to 13% and ranged from 6 to 13% for ISSR primers. Results indicated that ISSR markers were more efficient than RAPD markers, as they detected 25.57% polymorphic DNA bands compared to 21.31% polymorphism for RAPD markers.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Antibacterial activity; Antioxidant capacity; Flavonoids; ISSR; Phenolic compounds; RAPD
المشرفين على المادة: 0 (Antioxidants)
9007-49-2 (DNA)
0 (Flavonoids)
0 (Anti-Bacterial Agents)
تواريخ الأحداث: Date Created: 20231013 Date Completed: 20231103 Latest Revision: 20231103
رمز التحديث: 20231103
DOI: 10.1016/j.apradiso.2023.111034
PMID: 37832227
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-9800
DOI:10.1016/j.apradiso.2023.111034