دورية أكاديمية
أعرض في EDS

Multi-Method Quantification of Acetyl-Coenzyme A and Further Acyl-Coenzyme A Species in Normal and Ischemic Rat Liver.

التفاصيل البيبلوغرافية
العنوان: Multi-Method Quantification of Acetyl-Coenzyme A and Further Acyl-Coenzyme A Species in Normal and Ischemic Rat Liver.
المؤلفون: Tokarska-Schlattner M; University Grenoble Alpes, Inserm U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France., Zeaiter N; University Grenoble Alpes, Inserm U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France., Cunin V; University Grenoble Alpes, CNRS UMR 5525, Laboratory TIMC-Translational Microbiology, Evolution, Engineering (TREE), Service de Biochimie, Biologie Moléculaire et Toxicologie Environnementale, CHU Grenoble-Alpes, 38058 Grenoble, France., Attia S; University Grenoble Alpes, Inserm U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France., Meunier C; University Grenoble Alpes, CNRS UMR 5525, Laboratory TIMC-Translational Microbiology, Evolution, Engineering (TREE), Service de Biochimie, Biologie Moléculaire et Toxicologie Environnementale, CHU Grenoble-Alpes, 38058 Grenoble, France., Kay L; University Grenoble Alpes, Inserm U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France., Achouri A; University Grenoble Alpes, Inserm U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France., Hiriart-Bryant E; University Grenoble Alpes, Inserm U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France., Couturier K; University Grenoble Alpes, Inserm U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France., Tellier C; University Grenoble Alpes, Inserm U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France., El Harras A; University Grenoble Alpes, Inserm U1209 and CNRS UMR5309, Institute for Advanced Biosciences (IAB), 38058 Grenoble, France., Elena-Herrmann B; University Grenoble Alpes, Inserm U1209 and CNRS UMR5309, Institute for Advanced Biosciences (IAB), 38058 Grenoble, France., Khochbin S; University Grenoble Alpes, Inserm U1209 and CNRS UMR5309, Institute for Advanced Biosciences (IAB), 38058 Grenoble, France., Le Gouellec A; University Grenoble Alpes, CNRS UMR 5525, Laboratory TIMC-Translational Microbiology, Evolution, Engineering (TREE), Service de Biochimie, Biologie Moléculaire et Toxicologie Environnementale, CHU Grenoble-Alpes, 38058 Grenoble, France., Schlattner U; University Grenoble Alpes, Inserm U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France.; Institut Universitaire de France (IUF), 75231 Paris, France.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2023 Oct 06; Vol. 24 (19). Date of Electronic Publication: 2023 Oct 06.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Tandem Mass Spectrometry* , Acyl Coenzyme A*/metabolism, Rats ; Animals ; Acetyl Coenzyme A/analysis ; Chromatography, Liquid/methods ; Coenzyme A/analysis ; Ischemia ; Liver/metabolism
مستخلص: Thioesters of coenzyme A (CoA) carrying different acyl chains (acyl-CoAs) are central intermediates of many metabolic pathways and donor molecules for protein lysine acylation. Acyl-CoA species largely differ in terms of cellular concentrations and physico-chemical properties, rendering their analysis challenging. Here, we compare several approaches to quantify cellular acyl-CoA concentrations in normal and ischemic rat liver, using HPLC and LC-MS/MS for multi-acyl-CoA analysis, as well as NMR, fluorimetric and spectrophotometric techniques for the quantification of acetyl-CoAs. In particular, we describe a simple LC-MS/MS protocol that is suitable for the relative quantification of short and medium-chain acyl-CoA species. We show that ischemia induces specific changes in the short-chain acyl-CoA relative concentrations, while mild ischemia (1-2 min), although reducing succinyl-CoA, has little effects on acetyl-CoA, and even increases some acyl-CoA species upstream of the tricarboxylic acid cycle. In contrast, advanced ischemia (5-6 min) also reduces acetyl-CoA levels. Our approach provides the keys to accessing the acyl-CoA metabolome for a more in-depth analysis of metabolism, protein acylation and epigenetics.
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معلومات مُعتمدة: SYMER ANR-15-IDEX-02, EpiSPRM4, EpiSPRM5 Agence Nationale de la Recherche; Nomination 2018 and 2023 Institut Universitaire de France; Support for GEMELI facility Région Auvergne-Rhône-Alpes
فهرسة مساهمة: Keywords: HPLC; NMR; acetyl-CoA; acyl-CoA; fluorometric assay; liver ischemia; mass spectrometry; metabolomics; spectrophotometric assay; succinyl-CoA
المشرفين على المادة: 72-89-9 (Acetyl Coenzyme A)
0 (Acyl Coenzyme A)
SAA04E81UX (Coenzyme A)
تواريخ الأحداث: Date Created: 20231014 Date Completed: 20231101 Latest Revision: 20231101
رمز التحديث: 20231101
مُعرف محوري في PubMed: PMC10573920
DOI: 10.3390/ijms241914957
PMID: 37834405
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms241914957