دورية أكاديمية
Multi-Method Quantification of Acetyl-Coenzyme A and Further Acyl-Coenzyme A Species in Normal and Ischemic Rat Liver.
العنوان: | Multi-Method Quantification of Acetyl-Coenzyme A and Further Acyl-Coenzyme A Species in Normal and Ischemic Rat Liver. |
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المؤلفون: | Tokarska-Schlattner M; University Grenoble Alpes, Inserm U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France., Zeaiter N; University Grenoble Alpes, Inserm U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France., Cunin V; University Grenoble Alpes, CNRS UMR 5525, Laboratory TIMC-Translational Microbiology, Evolution, Engineering (TREE), Service de Biochimie, Biologie Moléculaire et Toxicologie Environnementale, CHU Grenoble-Alpes, 38058 Grenoble, France., Attia S; University Grenoble Alpes, Inserm U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France., Meunier C; University Grenoble Alpes, CNRS UMR 5525, Laboratory TIMC-Translational Microbiology, Evolution, Engineering (TREE), Service de Biochimie, Biologie Moléculaire et Toxicologie Environnementale, CHU Grenoble-Alpes, 38058 Grenoble, France., Kay L; University Grenoble Alpes, Inserm U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France., Achouri A; University Grenoble Alpes, Inserm U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France., Hiriart-Bryant E; University Grenoble Alpes, Inserm U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France., Couturier K; University Grenoble Alpes, Inserm U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France., Tellier C; University Grenoble Alpes, Inserm U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France., El Harras A; University Grenoble Alpes, Inserm U1209 and CNRS UMR5309, Institute for Advanced Biosciences (IAB), 38058 Grenoble, France., Elena-Herrmann B; University Grenoble Alpes, Inserm U1209 and CNRS UMR5309, Institute for Advanced Biosciences (IAB), 38058 Grenoble, France., Khochbin S; University Grenoble Alpes, Inserm U1209 and CNRS UMR5309, Institute for Advanced Biosciences (IAB), 38058 Grenoble, France., Le Gouellec A; University Grenoble Alpes, CNRS UMR 5525, Laboratory TIMC-Translational Microbiology, Evolution, Engineering (TREE), Service de Biochimie, Biologie Moléculaire et Toxicologie Environnementale, CHU Grenoble-Alpes, 38058 Grenoble, France., Schlattner U; University Grenoble Alpes, Inserm U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France.; Institut Universitaire de France (IUF), 75231 Paris, France. |
المصدر: | International journal of molecular sciences [Int J Mol Sci] 2023 Oct 06; Vol. 24 (19). Date of Electronic Publication: 2023 Oct 06. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2000- |
مواضيع طبية MeSH: | Tandem Mass Spectrometry* , Acyl Coenzyme A*/metabolism, Rats ; Animals ; Acetyl Coenzyme A/analysis ; Chromatography, Liquid/methods ; Coenzyme A/analysis ; Ischemia ; Liver/metabolism |
مستخلص: | Thioesters of coenzyme A (CoA) carrying different acyl chains (acyl-CoAs) are central intermediates of many metabolic pathways and donor molecules for protein lysine acylation. Acyl-CoA species largely differ in terms of cellular concentrations and physico-chemical properties, rendering their analysis challenging. Here, we compare several approaches to quantify cellular acyl-CoA concentrations in normal and ischemic rat liver, using HPLC and LC-MS/MS for multi-acyl-CoA analysis, as well as NMR, fluorimetric and spectrophotometric techniques for the quantification of acetyl-CoAs. In particular, we describe a simple LC-MS/MS protocol that is suitable for the relative quantification of short and medium-chain acyl-CoA species. We show that ischemia induces specific changes in the short-chain acyl-CoA relative concentrations, while mild ischemia (1-2 min), although reducing succinyl-CoA, has little effects on acetyl-CoA, and even increases some acyl-CoA species upstream of the tricarboxylic acid cycle. In contrast, advanced ischemia (5-6 min) also reduces acetyl-CoA levels. Our approach provides the keys to accessing the acyl-CoA metabolome for a more in-depth analysis of metabolism, protein acylation and epigenetics. |
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معلومات مُعتمدة: | SYMER ANR-15-IDEX-02, EpiSPRM4, EpiSPRM5 Agence Nationale de la Recherche; Nomination 2018 and 2023 Institut Universitaire de France; Support for GEMELI facility Région Auvergne-Rhône-Alpes |
فهرسة مساهمة: | Keywords: HPLC; NMR; acetyl-CoA; acyl-CoA; fluorometric assay; liver ischemia; mass spectrometry; metabolomics; spectrophotometric assay; succinyl-CoA |
المشرفين على المادة: | 72-89-9 (Acetyl Coenzyme A) 0 (Acyl Coenzyme A) SAA04E81UX (Coenzyme A) |
تواريخ الأحداث: | Date Created: 20231014 Date Completed: 20231101 Latest Revision: 20231101 |
رمز التحديث: | 20231101 |
مُعرف محوري في PubMed: | PMC10573920 |
DOI: | 10.3390/ijms241914957 |
PMID: | 37834405 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1422-0067 |
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DOI: | 10.3390/ijms241914957 |