دورية أكاديمية
أعرض في EDS

Genome-Wide DNA Methylation Profiling as Frontline Diagnostics for Central Nervous System Embryonal Tumors in Hong Kong.

التفاصيل البيبلوغرافية
العنوان: Genome-Wide DNA Methylation Profiling as Frontline Diagnostics for Central Nervous System Embryonal Tumors in Hong Kong.
المؤلفون: Tam OCH; Department of Paediatrics and Adolescent Medicine, The University of Hong Kong, Pok Fu Lam, Hong Kong., Ho RSL; Department of Pathology, Gleneagles Hospital, Wong Chuk Hang, Hong Kong., Chan S; Department of Paediatrics and Adolescent Medicine, The University of Hong Kong, Pok Fu Lam, Hong Kong., Li KKW; Department of Anatomical and Cellular Pathology, Chinese University of Hong Kong, Sha Tin, Hong Kong., Lam TL; Department of Pathology, Queen Elizabeth Hospital, Kowloon, Hong Kong., Cheung ETY; Department of Pathology, Queen Elizabeth Hospital, Kowloon, Hong Kong., Cheung OY; Department of Pathology, Queen Elizabeth Hospital, Kowloon, Hong Kong., Ho WWS; Department of Neurosurgery, Queen Mary Hospital, Pok Fu Lam, Hong Kong.; Department of Neurosurgery, Hong Kong Children's Hospital, Kowloon, Hong Kong., Cheng KKF; Department of Neurosurgery, Queen Mary Hospital, Pok Fu Lam, Hong Kong.; Department of Neurosurgery, Hong Kong Children's Hospital, Kowloon, Hong Kong., Shing MMK; Department of Paediatrics and Adolescent Medicine, Hong Kong Children's Hospital, Kowloon, Hong Kong., Ku DTL; Department of Paediatrics and Adolescent Medicine, Hong Kong Children's Hospital, Kowloon, Hong Kong., Chung BHY; Department of Paediatrics and Adolescent Medicine, The University of Hong Kong, Pok Fu Lam, Hong Kong., Yang W; Department of Paediatrics and Adolescent Medicine, The University of Hong Kong, Pok Fu Lam, Hong Kong., Chan GCF; Department of Paediatrics and Adolescent Medicine, The University of Hong Kong, Pok Fu Lam, Hong Kong.; Department of Paediatrics and Adolescent Medicine, Hong Kong Children's Hospital, Kowloon, Hong Kong., Ng HK; Department of Anatomical and Cellular Pathology, Chinese University of Hong Kong, Sha Tin, Hong Kong., Liu APY; Department of Paediatrics and Adolescent Medicine, The University of Hong Kong, Pok Fu Lam, Hong Kong.; Department of Paediatrics and Adolescent Medicine, Hong Kong Children's Hospital, Kowloon, Hong Kong.
المصدر: Cancers [Cancers (Basel)] 2023 Oct 07; Vol. 15 (19). Date of Electronic Publication: 2023 Oct 07.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101526829 Publication Model: Electronic Cited Medium: Print ISSN: 2072-6694 (Print) Linking ISSN: 20726694 NLM ISO Abbreviation: Cancers (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مستخلص: This paper examines the link between CNS tumor biology and heterogeneity and the use of genome-wide DNA methylation profiling as a clinical diagnostic platform. CNS tumors are the most common solid tumors in children, and their prognosis remains poor. This study retrospectively analyzed pediatric patients with CNS embryonal tumors in Hong Kong between 1999 and 2017, using data from the territory-wide registry and available formalin-fixed paraffin-embedded tumor tissue. After processing archival tumor tissue via DNA extraction, quantification, and methylation profiling, the data were analyzed by using the web-based DKFZ classifier (Molecular Neuropathology (MNP) 2.0 v11b4) and t-SNE analysis. Methylation profiles were deemed informative in 85 samples. Epigenetic data allowed molecular subgrouping and confirmed diagnosis in 65 samples, verified histologic diagnosis in 8, and suggested an alternative diagnosis in 12. This study demonstrates the potential of DNA methylation profiling in characterizing pediatric CNS embryonal tumors in a large cohort from Hong Kong, which should enable regional and international collaboration in future pediatric neuro-oncology research.
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معلومات مُعتمدة: 06171666 Health and Medical Research Fund, HKSAR
فهرسة مساهمة: Keywords: CNS tumors; diagnostics; methylation profiling; pediatrics
تواريخ الأحداث: Date Created: 20231014 Latest Revision: 20231030
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10571663
DOI: 10.3390/cancers15194880
PMID: 37835574
قاعدة البيانات: MEDLINE
الوصف
تدمد:2072-6694
DOI:10.3390/cancers15194880