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Glutamate decarboxylase-1 is essential for efficient acclimation of Arabidopsis thaliana to nutritional phosphorus deprivation.

التفاصيل البيبلوغرافية
العنوان: Glutamate decarboxylase-1 is essential for efficient acclimation of Arabidopsis thaliana to nutritional phosphorus deprivation.
المؤلفون: Benidickson KH; Department of Biology, Queen's University, Kingston, ON, K7L 3N6, Canada., Raytek LM; Department of Biology, Queen's University, Kingston, ON, K7L 3N6, Canada., Hoover GJ; Department of Plant Agriculture, University of Guelph, Guelph, ON, N1G 2W1, Canada., Flaherty EJ; Department of Plant Agriculture, University of Guelph, Guelph, ON, N1G 2W1, Canada., Shelp BJ; Department of Plant Agriculture, University of Guelph, Guelph, ON, N1G 2W1, Canada., Snedden WA; Department of Biology, Queen's University, Kingston, ON, K7L 3N6, Canada., Plaxton WC; Department of Biology, Queen's University, Kingston, ON, K7L 3N6, Canada.
المصدر: The New phytologist [New Phytol] 2023 Dec; Vol. 240 (6), pp. 2372-2385. Date of Electronic Publication: 2023 Oct 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley on behalf of New Phytologist Trust Country of Publication: England NLM ID: 9882884 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8137 (Electronic) Linking ISSN: 0028646X NLM ISO Abbreviation: New Phytol Subsets: MEDLINE
أسماء مطبوعة: Publication: Oxford : Wiley on behalf of New Phytologist Trust
Original Publication: London, New York [etc.] Academic Press.
مواضيع طبية MeSH: Arabidopsis*/metabolism , Arabidopsis Proteins*/genetics , Arabidopsis Proteins*/metabolism, Phosphorus/metabolism ; Glutamate Decarboxylase/genetics ; Glutamate Decarboxylase/metabolism ; Acclimatization ; Aminobutyrates/metabolism ; gamma-Aminobutyric Acid/metabolism ; Plant Roots/metabolism ; Phosphates/metabolism ; Gene Expression Regulation, Plant
مستخلص: Glutamate decarboxylase (GAD) is a Ca 2+ -calmodulin-activated, cytosolic enzyme that produces γ-aminobutyrate (GABA) as the committed step of the GABA shunt. This pathway bypasses the 2-oxoglutarate to succinate reactions of the tricarboxylic acid (TCA) cycle. GABA also accumulates during many plant stresses. We tested the hypothesis that AtGAD1 (At5G17330) facilitates Arabidopsis acclimation to Pi deprivation. Quantitative RT-PCR and immunoblotting revealed that AtGAD1 transcript and protein expression is primarily root-specific, but inducible at lower levels in shoots of Pi-deprived (-Pi) plants. Pi deprivation reduced levels of the 2-oxoglutarate dehydrogenase (2-OGDH) cofactor thiamine diphosphate (ThDP) in shoots and roots by > 50%. Growth of -Pi atgad1 T-DNA mutants was significantly attenuated relative to wild-type plants. This was accompanied by: (i) an > 60% increase in shoot and root GABA levels of -Pi wild-type, but not atgad1 plants, and (ii) markedly elevated anthocyanin and reduced free and total Pi levels in leaves of -Pi atgad1 plants. Treatment with 10 mM GABA reversed the deleterious development of -Pi atgad1 plants. Our results indicate that AtGAD1 mediates GABA shunt upregulation during Pi deprivation. This bypass is hypothesized to circumvent ThDP-limited 2-OGDH activity to facilitate TCA cycle flux and respiration by -Pi Arabidopsis.
(© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.)
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معلومات مُعتمدة: RGPIN/04476-2018 Natural Sciences and Engineering Research Council of Canada; RGPIN/03986-2020 Natural Sciences and Engineering Research Council of Canada; RGPIN/04928-2018 Natural Sciences and Engineering Research Council of Canada
فهرسة مساهمة: Keywords: Arabidopsis thaliana (thale cress); Pi starvation response; glutamate decarboxylase; metabolic bypasses; phosphorus nutrition; γ-aminobutyrate shunt
المشرفين على المادة: 27YLU75U4W (Phosphorus)
EC 4.1.1.15 (Glutamate Decarboxylase)
0 (Arabidopsis Proteins)
0 (Aminobutyrates)
56-12-2 (gamma-Aminobutyric Acid)
0 (Phosphates)
تواريخ الأحداث: Date Created: 20231014 Date Completed: 20231117 Latest Revision: 20231117
رمز التحديث: 20240628
DOI: 10.1111/nph.19300
PMID: 37837235
قاعدة البيانات: MEDLINE
الوصف
تدمد:1469-8137
DOI:10.1111/nph.19300