دورية أكاديمية
The effect of bone and analytical methods on the assessment of bone mineralization response to dietary phosphorus, phytase, and vitamin D in nursery pigs.
| العنوان: | The effect of bone and analytical methods on the assessment of bone mineralization response to dietary phosphorus, phytase, and vitamin D in nursery pigs. |
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| المؤلفون: | Williams HR; Department of Animal Sciences and Industry, College of Agriculture, Kansas State University, Manhattan, KS 66506-0201, USA., Chin TE; Department of Animal Sciences and Industry, College of Agriculture, Kansas State University, Manhattan, KS 66506-0201, USA., Tokach MD; Department of Animal Sciences and Industry, College of Agriculture, Kansas State University, Manhattan, KS 66506-0201, USA., Woodworth JC; Department of Animal Sciences and Industry, College of Agriculture, Kansas State University, Manhattan, KS 66506-0201, USA., DeRouchey JM; Department of Animal Sciences and Industry, College of Agriculture, Kansas State University, Manhattan, KS 66506-0201, USA., Goodband RD; Department of Animal Sciences and Industry, College of Agriculture, Kansas State University, Manhattan, KS 66506-0201, USA., Bergstrom JR; DSM Nutritional Products, Parsippany, NJ 07054, USA., Rahe MC; Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA., Siepker CL; Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA., Sitthicharoenchai P; Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA., Radke SL; Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA., Ensley SM; Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506-0201, USA., Gebhardt JT; Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506-0201, USA. |
| المصدر: | Journal of animal science [J Anim Sci] 2023 Jan 03; Vol. 101. |
| نوع المنشور: | Randomized Controlled Trial, Veterinary; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society of Animal Science Country of Publication: United States NLM ID: 8003002 Publication Model: Print Cited Medium: Internet ISSN: 1525-3163 (Electronic) Linking ISSN: 00218812 NLM ISO Abbreviation: J Anim Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Champaign, IL : American Society of Animal Science |
| مواضيع طبية MeSH: | Phosphorus, Dietary*/pharmacology , 6-Phytase*/pharmacology, Swine ; Animals ; Calcification, Physiologic ; Vitamin D/pharmacology ; Gastrointestinal Tract ; Diet/veterinary ; Vitamins/pharmacology ; DNA/pharmacology ; Phosphates/pharmacology ; Animal Feed/analysis ; Phosphorus ; Digestion |
| مستخلص: | A total of 360 pigs (DNA 600 × 241, DNA; initially 11.9 ± 0.56 kg) were used in a 28-d trial to evaluate the effects of different bones and analytical methods on the assessment of bone mineralization response to dietary P, vitamin D, and phytase in nursery pigs. Pens of pigs (six pigs per pen) were randomized to six dietary treatments in a randomized complete block design with 10 pens per treatment. Dietary treatments were designed to create differences in bone mineralization and included: (1) 0.19% standardized total tract digestibility (STTD) P (deficient), (2) 0.33% STTD P (NRC [2012] requirement) using monocalcium phosphate, (3) 0.33% STTD P including 0.14% release from phytase (Ronozyme HiPhos 2700, DSM Nutritional Products, Parsippany, NJ), (4) 0.44% STTD P using monocalcium phosphate, phytase, and no vitamin D, (5) diet 4 with vitamin D (1,653 IU/kg), and (6) diet 5 with an additional 50 µg/kg of 25(OH)D3 (HyD, DSM Nutritional Products, Parsippany, NJ) estimated to provide an additional 2,000 IU/kg of vitamin D3. After 28 d on feed, eight pigs per treatment were euthanized for bone (metacarpal, 2nd rib, 10th rib, and fibula), blood, and urine analysis. The response to treatment for bone density and ash was dependent upon the bone analyzed (treatment × bone interaction for bone density, P = 0.044; non-defatted bone ash, P = 0.060; defatted bone ash, P = 0.068). Thus, the response related to dietary treatment differed depending on which bone (metacarpal, fibula, 2nd rib, or 10th rib) was measured. Pigs fed 0.19% STTD P had decreased (P < 0.05) bone density and ash (non-defatted and defatted) for all bones compared to 0.44% STTD P, with 0.33% STTD P generally intermediate or similar to 0.44% STTD P. Pigs fed 0.44% STTD P with no vitamin D had greater (P < 0.05) non-defatted fibula ash compared to all treatments other than 0.44% STTD P with added 25(OH)D3. Pigs fed diets with 0.44% STTD P had greater (P < 0.05) defatted second rib ash compared to pigs fed 0.19% STTD P or 0.33% STTD P with no phytase. In summary, bone density and ash responses varied depending on bone analyzed. Differences in bone density and ash in response to P and vitamin D were most apparent with fibulas and second ribs. There were apparent differences in the bone ash percentage between defatted and non-defatted bone. However, differences between the treatments remain consistent regardless of the analytic procedure. For histopathology, 10th ribs were more sensitive than 2nd ribs or fibulas for the detection of lesions. (© The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
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| معلومات مُعتمدة: | Minnesota Pork Board |
| فهرسة مساهمة: | Keywords: bone mineralization; calcium; growth performance; nursery pigs; phosphorus; vitamin D Local Abstract: [plain-language-summary] Lameness is defined as impaired movement or deviation from normal gait. There are many factors that can contribute to lameness, including but not limited to: infectious disease, genetic and conformational anomaly, and toxicity that affects the bone, muscle, and nervous systems. Metabolic bone disease is another cause of lameness in swine production and can be caused by inappropriate levels of essential vitamins or minerals. To understand and evaluate bone mineralization, it is important to understand the differences in diagnostic results between different bones and analytical techniques. Historically, percentage bone ash has been used as one of the procedures to assess metabolic bone disease as it measures the level of bone mineralization; however, procedures and results vary depending on the methodology and type of bone measured. Differences in bone density and ash in response to dietary P and vitamin D were most apparent in the fibulas and second ribs. There were apparent differences in the percentage of bone ash between defatted and non-defatted bone; however, the differences between the treatments remain consistent regardless of the analytic procedure. For histopathology, 10th ribs were more sensitive than 2nd ribs or fibulas for detection of lesions associated with metabolic bone disease. |
| المشرفين على المادة: | 68439-86-1 (bone ash) 0 (Phosphorus, Dietary) EC 3.1.3.26 (6-Phytase) 1406-16-2 (Vitamin D) 0 (Vitamins) 9007-49-2 (DNA) 0 (Phosphates) 27YLU75U4W (Phosphorus) |
| تواريخ الأحداث: | Date Created: 20231014 Date Completed: 20231109 Latest Revision: 20231113 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC10635674 |
| DOI: | 10.1093/jas/skad353 |
| PMID: | 37837391 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1525-3163 |
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| DOI: | 10.1093/jas/skad353 |